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60-p3E_INTRON-EmptyRNAi
Plasmid#163380Purposep3E-Vector (Gateway R2-L3) for Tol2-kit cloning. Vector for performing RNAi (gene silencing - knockdown) in zebrafish via cloning and expression of synthetic miR(s).DepositorTypeEmpty backboneUseRNAiTagsExpressionMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
ZE18 pwnt8 ORF1/2 myc
Plasmid#17052DepositorInsertwnt8 ORF1/2 (wnt8a Zebrafish)
UseTags6xMycExpressionMutationPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
ZP44 Wnt8 5' orf2
Plasmid#16898DepositorInsertwnt8 5'orf2 (wnt8a Zebrafish)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
5UAS zfSynaptophysin:GFP-5UAS DsRedExpress
Plasmid#74317PurposeA fusion of zebrafish synaptophysin to GFP and DsRedExpress under the control of separate UAS cassettes; for dual-labeling of retinal ganglion cell (RGC) axon arbors in zebrafishDepositorInsertssynaptophysin
DsRedExpress
UseTagsEGFPExpressionMutationPromoter5UASAvailable sinceMay 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR5'_ubi:loxP-EGFP-loxP
Plasmid#27322DepositorInsertubiquitin:loxP-EGFP-loxP
UseMultisite gateway 5' entry vectorTagsExpressionMutationPromoterAvailable sinceApril 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-nCas9n-A71
Plasmid#125644Purposemicro-injection of poly-adenylated nCas9n RNADepositorInsertnCas9n
UseRna in vitro transcription vectorTagsExpressionMutationPromoterT3Available sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
8xGliBS-IVS2-mCherry-NLS-polyA-Tol2
Plasmid#84604PurposeTol2-based hedgehog signal reporter plasmid for zebrafish transgenesisDepositorInsertmCherry
UseZebrafish transgenesisTagsExpressionMutationPromoter8xGliBS-delta-crystallin minimal promoterAvailable sinceOct. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
ZE32 Prickle-1-GFP-pCS2+
Plasmid#17073DepositorInsertPrickle-1 (prickle1a Zebrafish)
UseZebrafish expressionTagsGFPExpressionMutationPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-kcnn2
Plasmid#164958PurposeZebrafish kcnn2 gene CDSDepositorInsertkcnn2 (kcnn2 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUAS-MEYFP
Plasmid#86372Purposemembrane targeted EYFP expression in zebrafishDepositorInsertEYFP
UseZebrafishTagsGAP43 membrane targetingExpressionMutationPromoterUASAvailable sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
14UAS zfPSD95:GFP
Plasmid#74314PurposeA fusion of zebrafish PSD95 to GFP under control of 14 UAS repeatsDepositorInsertzebrafish PSD95
UseTagsEGFPExpressionMutationY599H and I793S (please see depositor comment bel…PromoterUASAvailable sinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS-IndHom-Tol2-TetR-cryaa-dsRed
Plasmid#73204PurposeBAC targeting cassette for pIndigoBAC-536 with tetR selection marker and lens-specific transgenesis marker dsRedDepositorInsertdsRed
UseTagsExpressionMutationPromoterAvailable sinceMarch 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-zDrosha
Plasmid#171540PurposeEncodes Zebrafish Drosha proteinDepositorInsertdrosha (rnasen Zebrafish)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS-IndHom-Tol2-FRT-GalK-cryaa-dsRed
Plasmid#73203PurposeBAC targeting cassette for pIndigoBAC-536 with GalK selection marker and lens-specific transgenesis marker dsRedDepositorInsertdsRed
UseTagsExpressionMutationPromoterAvailable sinceSept. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS-TarHom-Tol2-FRT-GalK-cryaa-CFP
Plasmid#73200PurposeBAC targeting cassette for pTARBAC2 with GalK selection marker and lens-specific transgenesis marker CeruleanDepositorInsertCFP
UseTagsExpressionMutationPromoterAvailable sinceMarch 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUAS-MmTFP13xmyc
Plasmid#86371Purposemembrane targeted Tfp1 expression in zebrafishDepositorInsertmTfp1
UseZebrafishTags3xMyc and GAP43 membrane targetingExpressionMutationPromoterUASAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBS-TarHom-Tol2-FRT-GalK-cryaa-YFP
Plasmid#73198PurposeBAC targeting cassette for pTARBAC2 with GalK selection marker and lens-specific transgenesis marker CitrineDepositorInsertYFP
UseTagsExpressionMutationPromoterAvailable sinceMarch 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
