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Addgene
Showing: 361 - 380 of 674 results
  1. pBP Omomer

    Plasmid
    #113169
    Purpose
    Retroviral vector for expressing Omomer (tamoxifen inducible Omomyc), by DNA transfection or retroviral infection.
    Depositor
    Insert
    Omomer: a fusion contruct of Omomyc and MER (a mutant estrogen receptor, reponsive to tamoxifen) (MYC Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    E57T, E64I, R70Q, R71N
    Promoter
    Available since
    Aug. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
  2. qTAG-N-Solo-mStayGold-MYO1C

    Plasmid
    #227306
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the MYO1C locus. For membrane visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MYO1C (Addgene #227305)
    Depositor
    Insert
    MYO1C Homology Arms flanking a mStayGold Tag (MYO1C Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. qTAG-N-Solo-mScarlet-TUBA1B

    Plasmid
    #207764
    Purpose
    Donor template for mScarlet insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763
    Depositor
    Insert
    TUBA1B Homology Arms flanking a mScarlet Tag (TUBA1B Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
  4. Per2:iLuc

    Plasmid
    #160293
    Purpose
    target Per2 gene with a color-switching luciferase cassette in mouse genome and the transfection of the recombinant DNA will lead genomic recombination and make a knock-in allele in mouse ES cells.
    Depositor
    Insert
    Per2LUC
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    no promotor
    Available since
    Dec. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
  5. CDK1as_T2A_Zeo

    Plasmid
    #118596
    Purpose
    One of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and Zeocin resistance cassette flanked by Sleeping Beauty transposon.
    Depositor
    Insert
    CDK1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
  6. qTAG-C-moxGFP-Puro-H2BC11

    Plasmid
    #207758
    Purpose
    Donor template for moxGFP-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755
    Depositor
    Insert
    H2BC11 Homology Arms flanking a moxGFP-Puro Cassette (H2BC11 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 17, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
  7. qTAG-C-miRFP670nano3-Blast-H2BC11

    Plasmid
    #207762
    Purpose
    Donor template for miRFP670nano3-2A-Blast insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755
    Depositor
    Insert
    H2BC11 Homology Arms flanking a miRFP670nano3-Blast Cassette (H2BC11 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
  8. pAS_4xU6-PylT M15 (UUA) sfGFP 150TAA

    Plasmid
    #154775
    Purpose
    plasmid with 4xMma PylT (M15 mutant, UUA anticodon) cassette and ochre suppression reporter sfGFP 150 TAA stop, for transient transfection or stable, piggybac-mediated, integration
    Depositor
    Insert
    sfGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    150TAA in GFP reporter, UUA anticodon and G10A, …
    Promoter
    EF1
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
  9. pAS_4xhyb PylT A41AA C55A sfGFP 102TAG 150TAA

    Plasmid
    #154776
    Purpose
    plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and dual suppression reporter sfGFP 102TAG 150 TAA stop, for transient transfection or stable, piggybac-mediated, integration
    Depositor
    Insert
    sfGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    102TAG 150TAA in GFP reporter, hybrid PylT with A…
    Promoter
    EF1
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
  10. qTAG-C-mScarlet-Puro-H2BC11

    Plasmid
    #207761
    Purpose
    Donor template for mScarlet-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755
    Depositor
    Insert
    H2BC11 Homology Arms flanking a mScarlet-Puro Cassette (H2BC11 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 17, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
  11. qTAG-C-moxGFP-Blast-H2BC11

    Plasmid
    #207757
    Purpose
    Donor template for moxGFP-2A-Blast insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755
    Depositor
    Insert
    H2BC11 Homology Arms flanking a moxGFP-Blast Cassette (H2BC11 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 16, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
  12. qTAG-C-mNeon-Blast-TUBB4B

    Plasmid
    #207786
    Purpose
    Donor template for mNeon-2A-Blast insertion into the C-terminus of the TUBB4B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBB4B Addgene #207785
    Depositor
    Insert
    TUBB4B Homology Arms flanking a mNeon-Blast Cassette (TUBB4B Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
  13. pAS_4xhyb PylT A41AA C55A sfGFP 150TAG

    Plasmid
    #154772
    Purpose
    plasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid mutant A41AA C55A) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration
    Depositor
    Insert
    sfGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    150TAG in GFP reporter, hybrid PylT with A41AA an…
    Promoter
    EF1
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 361 - 380 of 674 results