Addgene

We narrowed to 635 results for: plko.1

Showing: 361 - 380 of 635 results

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pLKO.1C-shSCR

Plasmid

#139472

Purpose

Replacing the RSV promoter of pLKO.1 with the CMV promoter improves lentiviral production with third-generation viral packaging system.

Depositor

David Cobrinik

Article

Lee et al Biotechniques. 2020 Jun;68(6):349-352. d

Insert

shSCR

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1C-YFP

Plasmid

#139646

Purpose

Replacing the RSV promoter of pLKO.1 with the CMV promoter improves lentiviral production with third-generation viral packaging system.

Depositor

David Cobrinik

Article

Lee et al Biotechniques. 2020 Jun;68(6):349-352. d

Insert

stuffer

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

See Depositor comments

Promoter

CMV

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO_FOXA1_#2

Plasmid

#70096

Purpose

Expression of shRNA to human FOXA1, puromycin selection

Depositor

William Hahn

Article

Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

Insert

FOXA1 (FOXA1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

u6

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO_HOXB13_#2

Plasmid

#70094

Purpose

Expression of shRNA to human HOXB13, puromycin selection

Depositor

William Hahn

Article

Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

Insert

HOXB13 (HOXB13 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pLKO‐puro‐2A‐mdx4cv‐GFP

Plasmid

#187053

Purpose

fluorescent reporter for adenine base editing of mdx4cv point mutation

Depositor

Renzhi Han

Article

Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

Insert

GFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO-sh-hSC

Plasmid

#46896

Purpose

expression of scrambled shRNA

Depositor

Do-Hyung Kim

Article

Kim et al Mol Cell. 2012 Jun 29;46(6):833-46. doi:

Insert

scrambled

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only
pLKO-Tet-On-YAP1-shRNA1

Plasmid

#176846

Purpose

lentiviral vector for doxycycline-inducible human YAP1 knockdown

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

YAP1-Sh1 (YAP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO-Tet-On-YAP1-shRNA2

Plasmid

#176847

Purpose

lentiviral vector for doxycycline-inducible human YAP1 knockdown

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

YAP1-Sh2 (YAP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO-shBRCA1 #2

Plasmid

#44595

Depositor

Eros Lazzerini Denchi

Article

Okamoto et al Nature. 2013 Feb 6. doi: 10.1038/nature1

Insert

BRCA1 (Brca1 Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only
pLKO.puro_shGLS

Plasmid

#110335

Purpose

shGLS (Target CAACTGGCCAAATTCAGTC), silence human GLS gene, puromycin selection

Depositor

Sandra Martha Gomes Dias

Article

Dias et al Oncogene. 2020 Jan;39(3):690-702. doi: 1

Insert

GLS glutaminase (GLS Synthetic, Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6 (RNA PolIII)

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO-sh-hSH3BP4

Plasmid

#46895

Purpose

expression of shRNA against human SH3BP4

Depositor

Do-Hyung Kim

Article

Kim et al Mol Cell. 2012 Jun 29;46(6):833-46. doi:

Insert

SH3BP4 (SH3BP4 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

W92A (mutated SH3 domain)

Promoter

Available since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only
pLKO-sh-mSH3BP4

Plasmid

#46894

Purpose

expression of shRNA against mouse SH3BP4

Depositor

Do-Hyung Kim

Article

Kim et al Mol Cell. 2012 Jun 29;46(6):833-46. doi:

Insert

SH3BP4 (Sh3bp4 Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

W92A (mutated SH3 domain)

Promoter

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
tet-pLKO shMKP1.v1 puro

Plasmid

#87790

Purpose

Expresses an inducible short hairpin targeting human MKP1 sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shMKP1 (DUSP1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE tight

Available since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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tet-pLKO shMKP1.v2 puro

Plasmid

#87791

Purpose

Expresses an inducible short hairpin targeting human MKP1 sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shMKP1 (DUSP1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE tight

Available since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO-FOXM1-shRNA-81

Plasmid

#89495

Purpose

Lentiviral vector with constitutive expression of shRNA targeting human FOXM1.

Depositor

Adam Karpf

Insert

FOXM1 (FOXM1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 1, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO-FOXM1-shRNA-84

Plasmid

#89494

Purpose

Lentiviral vector with constitutive expression of shRNA targeting human FOXM1.

Depositor

Adam Karpf

Insert

FOXM1 (FOXM1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 1, 2017

Availability

Academic Institutions and Nonprofits only

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hgRNA-A21_pLKO-Hyg

Plasmid

#100559

Purpose

hgRNA-A21 in a lentiviral backbone

Depositor

George Church

Article

Kalhor et al Nat Methods. 2017 Feb;14(2):195-200. doi

Insert

hgRNA-A21

Use

CRISPR, Lentiviral, and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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hgRNA-A51_pLKO-Hyg

Plasmid

#100567

Purpose

hgRNA-A51 in a lentiviral backbone

Depositor

George Church

Article

Kalhor et al Nat Methods. 2017 Feb;14(2):195-200. doi

Insert

hgRNA-A51

Use

CRISPR, Lentiviral, and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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hgRNA-F21_pLKO-Hyg

Plasmid

#100564

Purpose

hgRNA-F21 in a lentiviral backbone

Depositor

George Church

Article

Kalhor et al Nat Methods. 2017 Feb;14(2):195-200. doi

Insert

hgRNA-F21

Use

CRISPR, Lentiviral, and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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hgRNA-A26_pLKO-Hyg

Plasmid

#100566

Purpose

hgRNA-A26 in a lentiviral backbone

Depositor

George Church

Article

Kalhor et al Nat Methods. 2017 Feb;14(2):195-200. doi

Insert

hgRNA-A26

Use

CRISPR, Lentiviral, and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge