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Plasmid#191242PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A31 and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
UseTagsnuclease A from S. aureus fused to the poly-Leu T…ExpressionBacterialMutationChanged Alanine 112 to Cysteine in SN, and predic…PromoterT7 promoterAvailable sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSN-A112C-CP8B1
Plasmid#191240PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-CP8B1 and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), CYP8B1 (CYP12) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
UseTagsnuclease A from S. aureus fused to the poly-Leu T…ExpressionBacterialMutationChanged Alanine 112 to Cysteine in SN, and predic…PromoterT7 promoterAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRC/CMV-TYK2VF/PA-VSV
Plasmid#139351PurposeExpression of the double mutant of TYK2-V678F-P1104ADepositorInsertTYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
UseTagsVSVExpressionMammalianMutationV362F-V678F-P1104APromoterCMVAvailable sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2-zfTpo
Plasmid#168198PurposeExpress zebrafish thrombopoietin (Tpo), mRNA synthesisDepositorInsertThrombopoietin (thpo Zebrafish)
UseVertebrate expression (zebrafish, chicken, xenopu…TagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRC/CMV-JAK1K908E-VSV
Plasmid#139357PurposeExpression of kinase-dead JAK1-K908E proteinDepositorInsertJAK1 cDNA with 300nt of 5' UTR (JAK1 Human)
UseTagsVSVExpressionMammalianMutationK908EPromoterCMVAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRC/CMV-JAK1P1084A-VSV
Plasmid#139358PurposeExpression of the catalytically impaired JAK1-P1084A mutant (corresponding to TYK2-P1104A)DepositorInsertJAK1 cDNA with 300nt of 5' UTR (JAK1 Human)
UseTagsVSVExpressionMammalianMutationP1084APromoterCMVAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
6-His-MBP-TEV-FnCpf1
Plasmid#90094PurposeBacterial expression plasmid for protein purificationDepositorInsertFnCpf1 (humanized)
UseTags3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…ExpressionBacterialMutationPromoterT7Available sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAc-His-zfTpo
Plasmid#64309PurposeExpress soluble His-tagged zebrafish thrombopoietin in insect cellsDepositorInsertThrombopoietin (thpo Zebrafish)
UseTags(His)6ExpressionInsectMutationThrombopoietin starts at AA37 (AA1-36 encode the …PromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZM3[I:FruR-L,I:RbsR-L]
Plasmid#60777PurposeContains PI driving expression FruR-L, and PI driving expression of RbsR-L.DepositorInsertsFruR-L
RbsR-L
UseTagsExpressionMutationLacI/GalR repressor chimera. LacI DBD with FruR L…PromoterAvailable sinceJuly 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
p420-3
Plasmid#71257Purposeluciferase reporter with 3 copies of the GM420 NFAT-AP-1 site in front of hGM-CSF -55 to +28 minimal promoterDepositorInsert3 copies of the GM420 NFAT-AP-1 site (CSF2 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterGM-CSF minimalAvailable sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-CLCb (EED/QQN)
Plasmid#47422DepositorInsertCLCb EED/QQN (CLTB Human)
UseTagsEGFPExpressionMammalianMutationEED changed to QQN, in aa20-41 regionPromoterCMVAvailable sinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSMALB-ATF4.5
Plasmid#155032PurposeFluorescent reporter for ATF4 translationDepositorInsertsUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSMALB-ATF4.12
Plasmid#155033PurposeFluorescent reporter for ATF4 translation, Negative ControlDepositorInsertsUseLentiviralTagsExpressionMammalianMutationuORF1 start codon mutatedPromoterAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSMALB-ATF4.14
Plasmid#155034PurposeFluorescent reporter for ATF4 translation, Positive ControlDepositorInsertsUseLentiviralTagsExpressionMammalianMutationuORF1 and uORF2 start codons mutatedPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVTHM-Bptf-sh28
Plasmid#83278PurposeshRNA targeting BPTFDepositorUseLentiviralTagsExpressionMutationPromoterTRE-H1Available sinceOct. 25, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCJ208
Plasmid#162681PurposeLidded variant of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) removing a seven-residue loop of PETase (Trp185:Phe191, PETase numbering) and replacing it with Gly251:Thr472 from MHETase; codon optimized for expression in E. coli K12, with C-terminal His tag.DepositorInsertPETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) incorporating the MHETase lid
UseTagsHisExpressionBacterialMutationReplacement of W185-F191 with G251-T472 from MHET…PromoterT7/lacAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCJ209
Plasmid#162682PurposeLidless variant of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) replacing Gly251:Thr472 of MHETase with the 7-residue loop (Trp185:Phe191, PETase numbering) of PETase , codon optimized for expression in E. coli K12, with C-terminal His tag.DepositorInsertMHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) with the lid removed
UseTagsHisExpressionBacterialMutationReplacement of G251-T472 with W185-F191 from PETa…PromoterT7/lacAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAG87 FAST
Plasmid#130719PurposeExpresses His-tagged FAST (also called YFAST) in bacteriaDepositorInsertFAST
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
Gucy1b2-IRES-nlsCre-IRES-taulacZ-FNF TV
Plasmid#105196Purposetargeting vector to generate a Gucy1b2-IRES-nlsCre-IRES-taulacZ mouse strain.DepositorInsertGucy1b2-IRES-nlsCre-IRES-taulacZ-FNF (Gucy1b2 Mouse)
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only