Skip to main content
Addgene
Showing: 361 - 380 of 565 results

  5. pET30b_OmpAT7Sav

    Plasmid
    #138589
    Purpose
    T7-tagged streptavidin with native C-terminus and periplasmic export signal OmpA; controlled by PT7 promoter; KanR, pBR322 ori
    Depositor
    Markus Jeschek
    Article
    Jeschek et al Nature. 2016 Sep 29;537(7622):661-665. d
    Insert
    T7-tagged streptavidin variant with native C-terminus and OmpA signal peptide for periplasmic export
    Use
    Synthetic Biology
    Tags
    OmpA export signal and T7 tag
    Expression
    Bacterial
    Mutation
    Promoter
    PT7
    Available since
    March 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    138589-plasmid-map-sequence-id-274488

    Enlarge

  10. RNA polymerase beta prime subunit FLAG

    Plasmid
    #102337
    Purpose
    Targeting plasmids for replacing the S. elongatus PCC 7942 genomic copy of Synpcc7942_1524 (Beta prime subunit of RNA polymerase) with a C-terminal FLAG tagged variant.
    Depositor
    Erin O'Shea
    Article
    Piechura et al Elife. 2017 Dec 14;6. pii: 32032. doi: 1
    Insert
    RNAP Beta Prime Subunit with C-terminal FLAG TAG
    Use
    Tags
    3x FLAG with 3X GSGS linker
    Expression
    Mutation
    Promoter
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    102337-plasmid-map-sequence-id-197499

    Enlarge

  12. pJin-290

    Plasmid
    #125718
    Purpose
    ABA-inducible split T7 gRNA for GFP vector using RNAPN d5-19 with CGG RNAP-C
    Depositor
    Bryan Dickinson
    Article
    Pu et al ACS Chem Biol. 2018 Feb 16;13(2):431-437
    Insert
    PCGG-gRNA GFP-off expression, FKBP-linker-T7 RNAPC, T7 RNAPN (d5-19)-linker-FRB
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    125718-plasmid-map-sequence-id-244348

    Enlarge

  13. pCS5

    Plasmid
    #55750
    Purpose
    Produces one half (beta) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.
    Depositor
    Matthew Bennett
    Article
    Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50
    Insert
    KanR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Residues 59-264 of KanR
    Promoter
    T7-lac
    Available since
    July 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    55750-plasmid-map-sequence-id-86407

    Enlarge

  15. pGDP4 ermC

    Plasmid
    #112884
    Purpose
    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.
    Depositor
    Gerard Wright
    Article
    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109
    Insert
    ermC (ermM Staphylococcus epidermis)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Plac
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112884-plasmid-map-sequence-id-219861

    Enlarge

  16. pCK300

    Plasmid
    #87766
    Purpose
    empty control plasmid, three terminators to place inserts between for insulation, ampR, pBR322 origin, lacI
    Depositor
    John Heap
    Article
    Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    None
    Available since
    May 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    87766-plasmid-map-sequence-id-177280

    Enlarge

  17. pGDP3 rmtB

    Plasmid
    #112880
    Purpose
    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.
    Depositor
    Gerard Wright
    Article
    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109
    Insert
    rmtB (RmtB Escherichia coli)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Pbla
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112880-plasmid-map-sequence-id-219870

    Enlarge

  18. pGDP3 cat

    Plasmid
    #112888
    Purpose
    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.
    Depositor
    Gerard Wright
    Article
    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109
    Insert
    cat (Brevibacillus brevis) (cat Brevibacillus brevis)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Pbla
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112888-plasmid-map-sequence-id-219887

    Enlarge

  19. pGDP1 vph

    Plasmid
    #112893
    Purpose
    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.
    Depositor
    Gerard Wright
    Article
    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109
    Insert
    vph
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Pbla
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112893-plasmid-map-sequence-id-219905

    Enlarge

  20. pGDP3 vatD

    Plasmid
    #112885
    Purpose
    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.
    Depositor
    Gerard Wright
    Article
    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109
    Insert
    vatD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pbla
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112885-plasmid-map-sequence-id-222210

    Enlarge

Showing: 361 - 380 of 565 results