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Plasmid#175279PurposeAAV vector mediating inducible expression of the NS1 gene of YFV-17DDepositorInsertNS1 (POLY Yellow fever virus strain 17D)
UseAAVTagsExpressionMutationPromotertight TREAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K11
Plasmid#121152PurposeMammalian expression of HA tagged ubiquitin with K11 only, other lysines mutated to argininesDepositorInsertUbiquitin C (UBC Human)
UseTagsExpressionMammalianMutationAll Ks apart from K11 mutated to RsPromoterAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K11R
Plasmid#121154PurposeMammalian expression of HA tagged ubiquitin K11RDepositorInsertUbiquitin C (UBC Human)
UseTagsExpressionMammalianMutationK11 mutated to an RPromoterAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-TRE-NS1-dTomato
Plasmid#175278PurposeAAV vector mediating inducible bicistronic expression of NS1 gene of YFV-17D and dTomatoDepositorInsertNS1; dTomato (POLY Yellow fever virus strain 17D)
UseAAVTagsdTomato (from bidirectional promoter)ExpressionMutationPromoterbidirectional TRE promoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-TRE-C-prM-E-NS1
Plasmid#175277PurposeAAV vector mediating inducible expression of 4 genes (the C-prM-E-NS1) of YFV-17DDepositorInsertC-prM-E-NS1 (POLY Yellow fever virus strain 17D)
UseAAVTagsExpressionMutationPromotertight TREAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
eGFP-PRM-5R
Plasmid#112155PurposeMammalian expression plasmid for PRM-5R in a vector expressing eGFP.DepositorInsertPRM-5R (ABL1 Human)
UseTagsExpressionMammalianMutationcontains only the PRM domain of Abl1PromoterCMVAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
K785R Smarca4-V5 IRES-puro
Plasmid#107059PurposeExpression of dominant-negative K785R Smarca4 (Brg1)-V5 fusion with IRES puromycin resistance.DepositorInsertSWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
UseLentiviralTagsIRES-puroR and V5ExpressionMammalianMutationK785RPromoterCAGAvailable sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K27R
Plasmid#121155PurposeMammalian expression of HA tagged ubiquitin K27RDepositorInsertUbiquitin C (UBC Human)
UseTagsExpressionMammalianMutationK27 mutated to an RPromoterAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIRESpuroN3xFLAG-His-CDK5RAP3
Plasmid#204555PurposeExpresses 3xFLAG-His-tagged CDK5RAP3 in mammalian cellsDepositorInsertCDK5RAP3 (AMY2A Human)
UseTagsExpressionMammalianMutationnonePromoterAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K6R
Plasmid#121153PurposeMammalian expression of HA tagged ubiquitin K6RDepositorInsertUbiquitin C (UBC Human)
UseTagsExpressionMammalianMutationK6 mutated to an RPromoterAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
HT107_pAAV_hSyn-DiO-SomQuasAr6a_EGFP-P2A-somCheRiff_HA
Plasmid#178826PurposeCre-on bicistronic expression of QuasAr6b-based Optopatch under an neuronal promoterDepositorInsertSomQuasAr6a_EGFP-P2A-somCheRiff_HA
UseAAV, Cre/Lox, and Mouse TargetingTagsEGFP; HAExpressionMammalianMutationPromoterhSynAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMAL-SH3(2)-5R
Plasmid#112089PurposeBacterial expression plasmid containing His and MBP tags for 5 repeats of the second SH3 domain from human NCK1.DepositorInsertSH3(2)-5R (NCK1 Human)
UseTagsHis6 and MBPExpressionBacterialMutationconstruct contains only repeats of the second SH3…PromoterLacAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIRESpuroN3xFLAG-UFSP2 C302A
Plasmid#204554PurposeExpresses 3xFLAG-tagged UFSP2 C302A in mammalian cellsDepositorInsertUFSP2 (UFSP2 Human)
UseTagsExpressionMammalianMutationC302APromoterAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
px551-mSncg-0.27kb-HA-SpCas9
Plasmid#153208PurposeTruncated Mouse gamma-synuclein (mSncg-0.27kb) promoter-mediates HA and Cas9 expression in retinal ganglion cellsDepositorInsertCas9
UseAAV and CRISPRTagsHAExpressionMammalianMutationPromoterTruncated mSncg (0.27kb)Available sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
HT111_Fsyn_FAS(Cre off)_QuasAr6a_Citrine
Plasmid#178824PurposeNeuronal expression (Cre-off) of an archaerhodopsin-derived genetically encoded voltage indicator QuasAr6b carrying a Citrine expression tagDepositorInsertQuasAr6a_citrine
UseCre/Lox and LentiviralTagscitrineExpressionMammalianMutationPromoterhSynAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNSSCP
Plasmid#183188PurposePACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; DivIVA-sfGFP expressed under TDepositorInsertsDivIVA and superfolder GFP fusion protein
SpmX450 and T7 181+ fusion protein
T7 RNAP 1-179 variant and SpmX450 fusion protein
mRFP1 and popZ fusion protein
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K27R-K29R
Plasmid#121156PurposeMammalian expression of HA tagged ubiquitin K27R and K29RDepositorInsertUbiquitin C (UBC Human)
UseTagsExpressionMammalianMutationK27 and K29 mutated to RsPromoterAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
HT116_pAAV_hSyn-DiO-SomQuasAr6b_EGFP
Plasmid#190879PurposeCre-on expression of soma-targeted QuasAr6b under an neuronal promoterDepositorInsertsoma-targeted QuasAr6b
UseAAVTagsEGFPExpressionMutationPromoterhSynAvailable sinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBac SpyTag-β2AR-SpyTag
Plasmid#135620PurposeExpression of SpyTag-B2AR in insect cellDepositorInsertBeta 2 adrenergic receptor (ADRB2 Human)
UseTagsHA-FLAG tag, Spytag, and eGFP-10xHis-R1D4 tagExpressionInsectMutationDeleted 1-24 aa, 366-413 aaPromoterAvailable sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
HT115_pAAV_hSyn-DiO-SomQuasAr6b_EGFP-P2A-somCheRiff_HA
Plasmid#178827PurposeCre-on bicistronic expression of QuasAr6b-based Optopatch under an neuronal promoterDepositorInsertSomQuasAr6a_EGFP-P2A-somCheRiff_HA
UseAAV, Cre/Lox, and Mouse TargetingTagsEGFP, HAExpressionMammalianMutationPromoterhSynAvailable sinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only