Coomassie Purity Stain of Recombinant Antibodies
Type
Protocol
...contaminant) Peak 2 (contaminant) Peak 3 (HC) Peak 4 (LC) Total Area HC + LC Area Purity AR0018 310.142 207.971...samples based on your sample’s HC + LC area. Example calculation: If the sample HC + LC Area is 18179.442 and...kDa and ~25 kDa corresponding to the heavy chain (HC) and light chain (LC) proteins, respectively. There...L)\over A}*100$$ Where: P = Purity, % H = Area of HC peak L = Area of LC peak A = Total area of all bands...routinely without any issues. Plot the mg/mL versus the HC + LC Area column for the IgG standards in Google ...