1. pX330-U6-Chimeric_BB-CBh-hSpCas9

   Plasmid

   #42230

   Purpose

   A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Has Service

   Cloning Grade DNA

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. px330-Kmt2b-1

   Plasmid

   #162535

   Purpose

   sgRNA targeting Kmt2b

   Depositor

   Amaia Lujambio

   Article

   Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

   Insert

   sgRNA targeting Kmt2b (Kmt2b Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   U6

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. px330-Kmt2c-4

   Plasmid

   #162534

   Purpose

   sgRNA targeting Kmt2c

   Depositor

   Amaia Lujambio

   Article

   Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

   Insert

   sgRNA targeting Kmt2c (Kmt2c Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   U6

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. px330-Alb-1

   Plasmid

   #162544

   Purpose

   sgRNA targeting Alb

   Depositor

   Amaia Lujambio

   Article

   Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

   Insert

   sgRNA targeting Alb (Alb Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   U6

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. px330-Alb-2

   Plasmid

   #162545

   Purpose

   sgRNA targeting Alb

   Depositor

   Amaia Lujambio

   Article

   Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

   Insert

   sgRNA targeting Alb (Alb Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   U6

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. px330-Alb-3

   Plasmid

   #162546

   Purpose

   sgRNA targeting Alb

   Depositor

   Amaia Lujambio

   Article

   Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

   Insert

   sgRNA targeting Alb (Alb Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   U6

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. px330-Alb-4

   Plasmid

   #162547

   Purpose

   sgRNA targeting Alb

   Depositor

   Amaia Lujambio

   Article

   Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

   Insert

   sgRNA targeting Alb (Alb Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   U6

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pX330-TP53-1

   Plasmid

   #121917

   Purpose

   Encodes sgRNA targeting exon 4 of TP53

   Depositor

   William Hahn

   Article

   Howard et al Cancer Res. 2019 Feb 12. pii: 0008-5472.

   Insert

   TP53 (TP53 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pX330-TP53-2

   Plasmid

   #121918

   Purpose

   Encodes sgRNA targeting exon 9 of TP53

   Depositor

   William Hahn

   Article

   Howard et al Cancer Res. 2019 Feb 12. pii: 0008-5472.

   Insert

   TP53 (TP53 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. px330-Pten-2

   Plasmid

   #162552

   Purpose

   sgRNA targeting Pten

   Depositor

   Amaia Lujambio

   Article

   Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

   Insert

   sgRNA targeting Pten (Pten Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   U6

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. px330-Apob-2

    Plasmid

    #162549

    Purpose

    sgRNA targeting Apob

    Depositor

    Amaia Lujambio

    Article

    Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

    Insert

    sgRNA targeting Apob (Apob Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. px330-Apob-1

    Plasmid

    #162548

    Purpose

    sgRNA targeting Apob

    Depositor

    Amaia Lujambio

    Article

    Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

    Insert

    sgRNA targeting Apob (Apob Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. px330-Apob-3

    Plasmid

    #162550

    Purpose

    sgRNA targeting Apob

    Depositor

    Amaia Lujambio

    Article

    Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

    Insert

    sgRNA targeting Apob (Apob Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. px330-Apob-4

    Plasmid

    #162551

    Purpose

    sgRNA targeting Apob

    Depositor

    Amaia Lujambio

    Article

    Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

    Insert

    sgRNA targeting Apob (Apob Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Cdk13_intron4_sg3_pX330

    Plasmid

    #127340

    Purpose

    sgRNA that cuts within intron 4 of mouse CDK13 genomic locus- guide #3

    Depositor

    Phil Sharp

    Article

    Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

    Insert

    Mouse Cdk13 Intron 4 Targeting sgRNA (Cdk13 Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    U6 Promoter

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pX330-U6-(negative selection module)-Chimeric_BB-CBh-hSpCas9

    Plasmid

    #223321

    Purpose

    It contains a ccdB-CMr negative selection module flanked by BpiI (BbsI) sites that allows excluding clones without correct insertion of sgRNA. Human codon optimized Cas9 expressing plasmid.

    Depositor

    Jonathan Arias

    Article

    Petruškevičiūtė et al Stem Cell Res. 2024 Dec 12:103628. doi:

    Inserts

    SpCas9 codon optimized for human (cas9 Human, Synthetic)
    chloramphenicol resistance protein
    CcdB
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge