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Plasmid#200272PurposeExpressing vinculin with co-localization labeler TurboID and fluorescence reporter VenusDepositorInsertVinculin_TurboID_Venus
UseTagsExpressionMammalianMutationPromoterT7Available sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EF1alpha-dCas9-p300_Blast
Plasmid#192653Purpose3rd generation lenti vector encoding dCas9-p300 with 2A Blast resistance markerDepositorInsertdCas9-p300
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A; H840A in Cas9PromoterEF1aAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-tetO-mCherry
Plasmid#70273PurposeLentiviral plasmid for tetracycline/doxycycline dependent expression of mCherryDepositorInsertmCherry
UseLentiviralTagsExpressionMammalianMutationPromotertetOAvailable sinceNov. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEaaK-NanR-J23113-pNanA-CadC-pCadBA-GFP
Plasmid#200277PurposeCharacterisation of the sialic acid-responsive circuit with fluorescent reporterDepositorInsertsCadC
NanR
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-3xP3-GFP
Plasmid#130277PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes 3xP3-GFP visible eye marker and multiple cloning site.DepositorInsert3xP3-GFP
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-MTS-EGFP
Plasmid#190270PurposeLentiviral conditional expression of mitochondrial-targeted eGFP in mammalian cellsDepositorInsertMTS-EGFP
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterTight TRE promoterAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZR069_CROPseq-sgPTPRC-CRISPRi
Plasmid#180271PurposeLentiviral CROPseq vector expressing PTPRC (CD45) targeting sgRNA for CRISPRiDepositorInsertPTPRC (CD45) sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-F-RAM-d2tTA-TRE-mKate2
Plasmid#140274PurposeF-RAM, the Fos-dependent reporterDepositorInsertmKate2
UseAAVTagsExpressionMutationPromoterTREAvailable sinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-N-RAM-d2tTA-TRE-mKate2
Plasmid#140275PurposeN-RAM, the Npas4-dependent reporterDepositorInsertmKate2
UseAAVTagsExpressionMutationPromoterTREAvailable sinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EF1alpha-dCas9-10xGCN4_Hygro
Plasmid#192651Purpose3rd generation lenti vector encoding dCas9-10xGCN4 (Suntag) with 2A Hygro resistance markerDepositorInsertdCas9-10xGCN4
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A and N863A in Cas9PromoterEF1aAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-3xP3-RFP
Plasmid#130276PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes 3xP3-RFP visible eye marker and multiple cloning site.DepositorInsert3xP3-RFP
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNuc-cis
Plasmid#202796PurposeRK2-based conjugative plasmid containing the TevSpCas9 dual-endonuclease for targeted bacterial killingDepositorInsertTevSpCas9, sgRNA cassette, oriT, CenArsHis
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EF1alpha-scFv-p65-HSF1-Blast
Plasmid#192652Purpose3rd generation lenti vector encoding scFV-p65-HSF1 with 2A Blast resistance markerDepositorInsertscFv-p65-HSF1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTRIP CMV GFP1-9
Plasmid#130271PurposeLentiviral expression vector for stable expression of GFP1-9DepositorInsertGFP 1-9 OPT
UseLentiviralTagsnoneExpressionMammalianMutationPromoterCMVAvailable sinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-FEN1hWT
Plasmid#35027DepositorInsertFEN1 (FEN1 Human)
UseAdenoviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDGE1108
Plasmid#202772PurposeCRISPR-KO in Arabidopsis thalianaDepositorInsertsFAST marker
pRPS5a:zCas9_ttriple
ccdB cassette (BsaI-excisable)
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDGE1109
Plasmid#202773PurposeCRISPR-KO with TRE2 exonuclease co-expression in Arabidopsis thalianaDepositorInsertsFAST marker
pRPS5a:TREX2-zCas9_ttriple
ccdB cassette (BsaI-excisable)
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDB364
Plasmid#110275PurposeN-terminal 10-His DsbC TEV-peptide fusionDepositorTypeEmpty backboneUseTags10-His, TEV cleavage peptide, and mature DsbC (i.…ExpressionBacterialMutationPromoterT7Available sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
hisSUMO-ZFP57
Plasmid#102716PurposeExpress DNA binding domain of human Zinc finger protein 57 in E. coliDepositorInsertZinc finger protein 57 (ZNF57 Human)
UseTagshisSUMOExpressionBacterialMutationPromoterT7Available sinceNov. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMVS-V1
Plasmid#183094PurposeBasic conjugative shuttle vector for Escherichia coli and Methanothermobacter thermautotrophicusDepositorInsertspME2001
thermostable neomycin phosphotransferase gene
UseBacterial propagation and conjugativeTagsExpressionMutationPromoterAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only