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Plasmid#153952PurposeGateway-compatible Entry vector, with insert of S CDS bearing a 24nt deletion from SARS-CoV-2 genomic analysis in Davidson et al. 2020DepositorInsertS (S SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJune 23, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 SARS-CoV-2 S 24nt-del_nostop
Plasmid#153953PurposeGateway-compatible Entry vector, with insert of S CDS bearing a 24nt deletion from SARS-CoV-2 genomic analysis in Davidson et al. 2020DepositorInsertS (S SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJune 9, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pαH-S-GSAS-D614G-K417N-E484K-N501Y
Plasmid#171750PurposeMammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-K417N-E484K-N501YDepositorInsertSpike (S-GSAS-D614G-K417N-E484K-N501Y variant) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsHRV3C cleavage site, 8X His tag, 2X Strep-Tag IIExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…PromoterAvailable sinceJuly 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pISlin41w(s) (lin-41 microRNA wt reporter)
Plasmid#11906DepositorInsertlin-41 miRNA target sequence (lin-41 Nematode)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceApril 27, 2007AvailabilityAcademic Institutions and Nonprofits only -
pISlin41m(s) (lin-41 microRNA mut reporter)
Plasmid#11907DepositorInsertlin-41 miRNA target sequence mut (lin-41 Nematode)
UseLuciferaseTagsExpressionMammalianMutationpoint mutations in miRNA complementary sitePromoterAvailable sinceApril 27, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET-22b(+)_PelB-BN00H3-S-FLAG-His
Plasmid#218038PurposeRESOLUTE SLC binder expression plasmidDepositorInsertBN00H3-S
UseTags1xFLAG 10xHis and PelBExpressionBacterialMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Ras-LOCKR-S R89L (negative control)
Plasmid#216497PurposeFRET-based reporter for Ras activity, untargeted, negative controlDepositorInsertRas-LOCKR-S R89L negative control
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-2P-Omicron-BA.5
Plasmid#213828PurposeMammalian cell expression of SARS-CoV-2 Spike protein of OMICRON.2P.BA5 variantDepositorInsertpαH-S-GSAS/Omicron-2P-BA5 (S )
UseTagsHRV 3C cleavage site (before tags) 8X His tag 2X…ExpressionMammalianMutationG339D,R346T,S371F,S373P,S375F,T376A,D405N,R408S,K…PromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
GAL4-Creb5 (1- 128) C18/C23 S
Plasmid#195033PurposeGAL4-Creb5 fusion construct (N-terminus of Creb5,128aa, with C18/C23 S mutation)DepositorInsertGAL4-Creb5 fusion construct (N-terminus of Creb5,128aa, with C18/C23 S mutation) (CREB5 Bovine)
UseTagsExpressionMammalianMutationC18/C23 SPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
GABA(A)R, Gamma2L/S scFv [N452/30]
Plasmid#206785PurposeMammalian Expression of GABA(A)R, Gamma2L/S scFV. Derived from hybridoma N452/30 scFv.DepositorInsertGABA(A)R, Gamma2L/S (Homo sapiens) recombinant scFV (GABRG2 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
GABA(A)R, Gamma2L/S scFv [N452/73]
Plasmid#190555PurposeMammalian Expression of GABA(A)R, Gamma2L/S scFV. Derived from hybridoma N452/73.DepositorInsertGABA(A)R, Gamma2L/S (Homo sapiens) recombinant scFV (GABRG2 Mouse)
UseTagsHA, Sortase, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
GABA(A)R, Gamma2L/S scFv [N452/81]
Plasmid#190556PurposeMammalian Expression of GABA(A)R, Gamma2L/S scFV. Derived from hybridoma N452/81.DepositorInsertGABA(A)R, Gamma2L/S (Homo sapiens) recombinant scFV (GABRG2 Mouse)
UseTagsHA, Sortase, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-miRFP670-eDHFR(69K6)-G-alpha-s
Plasmid#172105PurposeMammalian expression of Gαs fused to miRFP670-eDHFR(69K6)DepositorInsertmiRFP670-eDHFR(69K6)-Gαs (GNAS Human)
UseTagsExpressionMammalianMutationGαs: C3SPromoterCAGAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-EMMI-027 mCherry(S-T)//Esp3I
Plasmid#130229PurposeEMMA Kit # 1000000119 extensionDepositorInsertmCherry
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBabe 3XFLAG-S/T3A-GATA6-3XAU1 puro
Plasmid#72611PurposeFor mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with triple alanine mutations on aminoacids#33,#34 and #37DepositorInsertGATA binding protein 6 (GATA6 Human)
UseRetroviralTags3XAU1 and 3XFLAGExpressionMammalianMutationchanged serine33, threonine34 and serine37 to ala…PromoterAvailable sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn NS34a-msGFP-'S-mito
Plasmid#158768PurposeLentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.DepositorInsertNS3/4a protease and msGFP
UseLentiviralTagsExpressionMutationFlexible linkers between the protease and msGFP a…PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-s-IRESWT-Gbetagamma ONE-GO
Plasmid#204340PurposeGPCR/G protein BRET biosensorDepositorInsertGalpha-s/Gbetagamma ONE-GO
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE
Plasmid#51509PurposeCan be used to generate AAV virus that will express cytoplasmic tdTomato and presynaptic (synaptophysin-fused) EGFP in the presence of Cre in neurons from the synapsin promoterDepositorHas ServiceAAV1InserttdTomato-T2A-SypEGFP
UseAAVTagsExpressionMutationPromoterphSyn1Available sinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHBS1505 [IBB-GFP-mCherry3E]-[BFP-TDP43 FYW-S]
Plasmid#133329PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationAll FYW to S in CTD of full-length TDP43PromoterAvailable sinceNov. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1504 [IBB-GFP-mCherry3E]-[BFP-TDP43 F-S]
Plasmid#133328PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationAll F to S in CTD of full-length TDP43PromoterAvailable sinceNov. 6, 2019AvailabilityAcademic Institutions and Nonprofits only