-
Plasmid#174026PurposeThis plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.DepositorInsertp2A-QF2
UseZebrafish knock-in taggingTagsExpressionMutationPromoterAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC19-TgKI-MCS-p2A-Venus-PEST-MCS-polyA
Plasmid#174030PurposeThis plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.DepositorInsertp2A-Venus-PEST
UseZebrafish knock-in taggingTagsExpressionMutationPromoterAvailable sinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC19-MAFA-PQR-NLS-tdTomato-P2A-puro-PGK-puro
Plasmid#121174PurposeDonor template for CRISPR-Cas9 mediated generation of MAFA-NLS-tdTomato reporter cell linesDepositorInsertMAFA-PQR-NLS-tdTomato-P2A-Puro-PGK-Puro
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC19-GCG-T2A-NLS-EmGFP-P2A-puro-PGK-puro
Plasmid#121175PurposeDonor template for CRISPR-Cas9 mediated generation of GCG-NLS-EmGFP reporter cell linesDepositorInsertGCG-T2A-NLS-EmGFP-P2A-Puro-PGK-Puro
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC19_mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP
Plasmid#195505PurposeT-REX17 donor plasmid to generate locus specific integration of mRuby followed by 3xSV40 poly(A) into Exon 1 of human lncRNA T-REX17DepositorInsertpT-REX-mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP_T-REX(Ex1)
UseCRISPR; Donor vectorTagsmRuby-3xFLAG-NLS-3xSV40-poly(A)ExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC18 Apln5-HA-FlpO-pA-LoxP-PGK-Neo-pA-LoxP-Apln3 (SO89)
Plasmid#159222PurposeCan be used to target the mouse Apln locus in mouse eggs or ES cells, in order to drive the mosaic expression of the protein HA-NLS-FlpODepositorInsertApln 5'-FlpO-WPRE-Sv40pA-LoxP-PGK-Neo-pA-LoxP-Apln
UseTagsExpressionMammalianMutationPromoterAplnAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC57-ITR2-H1-GFP sgRNA Target 3- eCMV- S.pyoegenes-3XFLAG-SV40NLS-ITR2
Plasmid#210739PurposeCoding for Sp Cas9 alongside Sp sgRNA targeting GFP Target 3DepositorInsertsUseCRISPRTags3xFLAG, SV40 NLS, PolyA signalExpressionMammalianMutationPromoterH1 and eCMVAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-ITR2-H1-GFP sgRNA Target 1- eCMV- S.pyoegenes-3XFLAG-SV40NLS-ITR2
Plasmid#210737PurposeCoding for Sp Cas9 alongside Sp sgRNA targeting GFP Target 1DepositorInsertsUseCRISPRTags3xFLAG, SV40 NLS, PolyA signalExpressionMammalianMutationPromoterH1 and eCMVAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-ITR2-H1- sgCTG Bilbo -eCMV-CjSpD8A-3XFLAG-SV40 NLS-ITR2
Plasmid#210749PurposeCoding for CjSp D8A alongside Bilbo sgRNA targeting CAG repeatsDepositorInsertsCjSp D8A Cas9
Bilbo sgCAG
UseCRISPRTags3xFLAG, SV40 NLS, PolyA signalExpressionMammalianMutationD8A, N-terminal Cj Cas9, C-terminal Sp Cas9PromoterH1 and eCMVAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-ITR2-H1-GFP sgRNA Target 2- eCMV- S.pyoegenes-3XFLAG-SV40NLS-ITR2
Plasmid#210738PurposeCoding for Sp Cas9 alongside Sp sgRNA targeting GFP Target 2DepositorInsertsUseCRISPRTags3xFLAG, SV40 NLS, PolyA signalExpressionMammalianMutationPromoterH1 and eCMVAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-ITR2-H1-GFP sgRNA Target 4- eCMV- S.pyoegenes-3XFLAG-SV40NLS-ITR2
Plasmid#210740PurposeCoding for Sp Cas9 alongside Sp sgRNA targeting GFP Target 4DepositorInsertsUseCRISPRTags3xFLAG, SV40 NLS, PolyA signalExpressionMammalianMutationPromoterH1 and eCMVAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-ITR2-H1-GFP sgRNA Merry Target 3- eCMV- SaSp-3XFLAG-SV40NLS-ITR2
Plasmid#210743PurposeCoding for SaSp Cas9 alongside Merry sgRNA targeting GFP Target 3DepositorInsertsSaSp Cas9
Merry sgRNA GFP Target 3
UseCRISPRTags3xFLAG, SV40 NLS, PolyA signalExpressionMammalianMutationN-terminal Sa Cas9, C-terminal Sp Cas9PromoterH1 and eCMVAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-ITR2-H1-GFP sgRNA Merry Target 4- eCMV- SaSp-3XFLAG-SV40NLS-ITR2
Plasmid#210744PurposeCoding for SaSp Cas9 alongside Merry sgRNA targeting GFP Target 4DepositorInsertsSaSp Cas9
Merry sgRNA GFP Target 4
UseCRISPRTags3xFLAG, SV40 NLS, PolyA signalExpressionMammalianMutationN-terminal Sa Cas9, C-terminal Sp Cas9PromoterH1 and eCMVAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-ITR2-H1-GFP sgRNA Gollum Target 3- eCMV- SaSp-3XFLAG-SV40NLS-ITR2
Plasmid#210747PurposeCoding for SaSp Cas9 alongside Gollum sgRNA targeting GFP Target 3DepositorInsertsSaSp Cas9
Gollum sgRNA GFP Target 3
UseCRISPRTags3xFLAG, SV40 NLS, PolyA signalExpressionMammalianMutationN-terminal Sa Cas9, C-terminal Sp Cas9PromoterH1 and eCMVAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-ITR2-H1-GFP sgRNA Gollum Target 4- eCMV- SaSp-3XFLAG-SV40NLS-ITR2
Plasmid#210748PurposeCoding for SaSp Cas9 alongside Gollum sgRNA targeting GFP Target 4DepositorInsertsSaSp Cas9
Gollum sgRNA GFP Target 4
UseCRISPRTags3xFLAG, SV40 NLS, PolyA signalExpressionMammalianMutationN-terminal Sa Cas9, C-terminal Sp Cas9PromoterH1 and eCMVAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-ITR2-H1-GFP sgRNA Gollum Target 2- eCMV- SaSp-3XFLAG-SV40NLS-ITR2
Plasmid#210746PurposeCoding for SaSp Cas9 alongside Gollum sgRNA targeting GFP Target 2DepositorInsertsSaSp Cas9
Gollum sgRNA GFP Target 2
UseCRISPRTags3xFLAG, SV40 NLS, PolyA signalExpressionMammalianMutationN-terminal Sa Cas9, C-terminal Sp Cas9PromoterH1 and eCMVAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-ITR2-H1-GFP sgRNA Merry Target 1- eCMV- SaSp-3XFLAG-SV40NLS-ITR2
Plasmid#210741PurposeCoding for SaSp Cas9 alongside Merry sgRNA targeting GFP Target 1DepositorInsertsSaSp Cas9
Merry sgRNA GFP Target 1
UseCRISPRTags3xFLAG, SV40 NLS, PolyA signalExpressionMammalianMutationN-terminal Sa Cas9, C-terminal Sp Cas9PromoterH1 and eCMVAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-ITR2-H1-GFP sgRNA Gollum Target 1- eCMV- SaSp-3XFLAG-SV40NLS-ITR2
Plasmid#210745PurposeCoding for SaSp Cas9 alongside Gollum sgRNA targeting GFP Target 1DepositorInsertsSaSp Cas9
Gollum sgRNA GFP Target 1
UseCRISPRTags3xFLAG, SV40 NLS, PolyA signalExpressionMammalianMutationN-terminal Sa Cas9, C-terminal Sp Cas9PromoterH1 and eCMVAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-ITR2-H1-GFP sgRNA Merry Target 2- eCMV- SaSp-3XFLAG-SV40NLS-ITR2
Plasmid#210742PurposeCoding for SaSp Cas9 alongside Merry sgRNA targeting GFP Target 2DepositorInsertsSaSp Cas9
Merry sgRNA GFP Target 2
UseCRISPRTags3xFLAG, SV40 NLS, PolyA signalExpressionMammalianMutationN-terminal Sa Cas9, C-terminal Sp Cas9PromoterH1 and eCMVAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-ITR2-H1- sgCTG Gollum-eCMV-SaSpD10A (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2
Plasmid#210735PurposeCoding for SaSp D10A Cas9 alongside Gollum sgRNA targeting CAG repeatsDepositorInsertsSaSp D10A Cas9
Gollum sgCAG
UseCRISPRTags3xFLAG, SV40 NLS, PolyA signalExpressionMammalianMutationD10A, N terminal Sa Cas9, C-terminal Sp Cas9PromoterH1 and eCMVAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-ITR2-H1- sgCTG Merry-eCMV-SaSpD10A+linker (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2
Plasmid#210736PurposeCoding for SaSp D10A Cas9 alongside Merry sgRNA targeting CAG repeatsDepositorInsertsSaSp D10A Cas9
Merry sgCAG
UseCRISPRTags3xFLAG, SV40 NLS, PolyA signalExpressionMammalianMutationD10A, N-terminal Sa Cas9, C-terminal Sp Cas9PromoterH1 and eCMVAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOK12
Plasmid#49789PurposeE. coli cloning vector (KanR, low copy, blue/white selection)DepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterlacZAvailable sinceJune 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUK21
Plasmid#49788PurposeE. coli cloning vector (KanR, high copy, blue/white selection, M13 IR)DepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterlacZAvailable sinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRL648
Plasmid#70283PurposepUC plasmid with inverted polylinker flanking PpsbA-Km markerDepositorTypeEmpty backboneUseBacterial shuttle vectorTagsExpressionMutationPromoterAvailable sinceMay 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHC_DYOLacI-R
Plasmid#102922PurposepUC-19 derivative reporter plasmid for the screening of Transcription Factors (TFs) carrying the DNA-binding domain of LacIDepositorInsertsfGFP
UseTagsExpressionMutationPromoterAvailable sinceAug. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSCVsin-PGK-PURO
Plasmid#35618DepositorInsertPuro
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMSCVsin-PGK-GFP
Plasmid#35615DepositorInsertGFP
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pD864-LacZ backbone
Plasmid#109384PurposeEmpty plasmid of the pD864-LacZ construct. This is high copy (pUC), AmpRDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
EF1a-EGFP (no signal control)
Plasmid#170167PurposeMammalian Expression of no signal controlDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationn/aPromoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
EF1a-EGFP-SKL
Plasmid#170166PurposeMammalian Expression of Ef1a-EGFP-SKLDepositorInsertEGFP-SKL
UseTagsExpressionMammalianMutationSKLPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
w+attB
Plasmid#30326DepositorInsertphiC31 attB, hsp70::w
UseDrosophila transgenesisTagsExpressionMutationPromoterAvailable sinceJuly 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
EF1a-EGFP-RSKL
Plasmid#170165PurposeMammalian Expression of Ef1a-EGFP-RSKLDepositorInsertEGFP-RSKL
UseTagsExpressionMammalianMutationRSKLPromoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
EF1a-EGFP-KANL
Plasmid#170164PurposeMammalian Expression of Ef1a-EGFP-KANLDepositorInsertEGFP-KANL
UseTagsExpressionMammalianMutationKANLPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
EF1a-ABCD3-RFP-Puro
Plasmid#170168PurposeMammalian Expression of Ef1a-ABCD3-RFP-PuroDepositorInsertABCD3-RFP-Puro
UseTagsExpressionMammalianMutationn/aPromoterAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTU1-A_UK_amilCP
Plasmid#204043PurposeType TU1-A backbone with kanamycin resistance and pUC ORI. amilCP dropout vector.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationNonePromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSAH031
Plasmid#90330PurposePromoterless iLOV gene for recombineering, flanked by BsmbI restriction sites.DepositorInsertiLOV
UseTagsNoneExpressionMutationPromoterNoneAvailable sinceJuly 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTU2-b_UrK_amilCP
Plasmid#204065PurposeType TU2-b backbone with kanamycin resistance and pUC-bom-rop ORI. amilCP dropout vector.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationNonePromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTU1-A_UrK_amilCP
Plasmid#204044PurposeType TU1-A backbone with kanamycin resistance and pUC-bom-rop ORI. amilCP dropout vector.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationNonePromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL0_152 [pBP1 ORI]
Plasmid#210674PurposeLevel 0 partDepositorInsertpBP1 ORI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
StarShip01
Plasmid#164505PurposeTo generate site specific attP-GA integrant cell lines upon co-delivery with BxbI. Contains T7-HaloTag-PDGFR_TM-T2A-Puro.DepositorTypeEmpty backboneUseBxbi site specific integrationTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
StarShip02
Plasmid#164506PurposeTo generate site specific attP-GA integrant cell lines upon co-delivery with BxbI. Contains IRES(Myb)-T7-Halo-PDGFR_TM-T2A-Puro.DepositorTypeEmpty backboneUseBxbi site specific integrationTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
StarShip03
Plasmid#164507PurposeTo generate site specific attP-GA integrant cell lines upon co-delivery with BxbI. Contains IRES2-T7-Halo-PDGFR_TM-T2A-Puro.DepositorTypeEmpty backboneUseBxbi site specific integrationTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pExp-RBD-CHis
Plasmid#195002PurposeProduction of SARS-CoV2 receptor binding domain in E. coli with C-terminal Avi-tagDepositorInsertRBD (S SARS-CoV-2)
UseTagsHis8ExpressionBacterialMutationPromoterT7lacAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
Sperm whale Mb 64Y68F in pVP80K
Plasmid#86956PurposeIncreases expression of sperm whale myoglobin protein for making apomyoglobin as a reagent for measuring rates of hemin dissociation (as opposed to previous construct in pUC).DepositorInsertMb 64Y68F
UseTagsExpressionBacterialMutation64Y68FPromoterT5Available sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-dSa-VPR
Plasmid#99651PurposeExpresses tripartite VP64-p65-RTA activator fused to C term. of dead Sa Cas9DepositorInsertdCas9
UseAAVTagsVP64-p65-RTA activatorExpressionMutationdead Cas9PromoterAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pExp-His-zBasic-RBD-Avi
Plasmid#195000PurposeProduction of SARS-CoV2 receptor binding domain in E. coli with C-terminal Avi-tagDepositorInsertRBD (S SARS-CoV-2)
UseTagsAvi-tag and His8-ZbasicExpressionBacterialMutationPromoterT7lacAvailable sinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pINT3
Plasmid#24778DepositorTypeEmpty backboneUseIntegrating vectorTagsExpressionMutationPromoterAvailable sinceAug. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV-cFOS-FRB-VP16-P2A-EGFP
Plasmid#210510Purposeexpresses cFOS-FRB-VP16 and EGFP component in rat hippocampal neuron cellsDepositorInsertFRB-VP16-P2A-EGFP
UseTagsExpressionMammalianMutationPromotercFosAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pULZ
Plasmid#169813PurposeProduction of circular recombinant RNA in Escherichia coli using viroid scaffolds, RNA precursor expression.DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
T7-3e5
Plasmid#106990PurposeT7 transcription template for 3e5DepositorInsert3e5
UseTagsExpressionMutationPromoterAvailable sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only