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Showing: 721 - 760 of 1147 results

  8. pMV7 lenti pEF-rTetR(SE-G72P)-3XFLAG-CACO1(Zn-C2H2_12)-T2A-mCherry-BSD-WPRE

    Plasmid
    #188753
    Purpose
    Lentiviral backbone containing CACO1 Zn-C2H2_12 domain as a fusion with rTetR(SE-G72P)-3XFLAG
    Depositor
    Michael Bassik, Lacra Bintu
    Article
    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038
    Insert
    CACO1 Zn-C2H2_12 (CALCOCO1 Human)
    Use
    Lentiviral
    Tags
    rTetR(SE-G72P)-3xFLAG
    Expression
    Mammalian
    Mutation
    Promoter
    pEF1a
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    188753-plasmid-map-sequence-id-371427

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  16. pLV-pGK-Nluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro

    Plasmid
    #115684
    Purpose
    NanoLuc-Firefly Luciferase fusion with constitutive nuclear expression for calibration of Nanoluc signal to Firefly Luciferase signal. H2B-mCherry marks chromatin with red fluorescence.
    Depositor
    David Suter
    Article
    Mandic et al Methods. 2017 May 1;120:65-75. doi: 10.1
    Insert
    Nluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    115684-plasmid-map-sequence-id-224735

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  17. LA001: pMVP (L3-L2) P2A-mCherry + WPRE

    Plasmid
    #121774
    Purpose
    pMVP L3-L2 entry plasmid, contains mCherry-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term mCherry linked by P2A to gene of interest in lentivirus vectors.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    P2A-mCherry + WPRE
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121774-plasmid-map-sequence-id-237269

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  21. TDP-REGv1(mCherry-FLAG) in pTwist-CMV

    Plasmid
    #216165
    Purpose
    Expression of mCherry specifically in cells with TDP-43 knockdown. Uses AARS1-derived cryptic exon to control mCherry expression. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Article
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    mCherry with C-terminal FLAG tag
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216165-plasmid-map-sequence-id-437747

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  26. pSLQ8629 pHR-HREYBTATA-RR12EE345L-C term (406) dLbCpf1-2XNLS-miniVPR-mCherry-EFS-Puro-WPRE

    Plasmid
    #140227
    Purpose
    Hypoxia-inducible expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to miniVPR. Constitutive EFS-driven puromycin resistance.
    Depositor
    Stanley Qi
    Article
    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2
    Insert
    zipper with dLbCpf1 (Cas12a) C terminal half (406) fused to miniVPR
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    HRE (Hypoxia inducible)
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    140227-plasmid-map-sequence-id-291996

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  28. pLV-hCdt1(1-100)ΔCy-mCherry-P2A-mVenus-hGeminin(1-110)-IRES-Blast

    Plasmid
    #193139
    Purpose
    Expresses bicistronic P2A construct of C-CRL4Cdt2 reporter(hCdt1(1-100)ΔCy-mCherry-) and APC/C reporter (hGeminin(1-110)) with blasticidin resistance
    Depositor
    Tobias Meyer
    Article
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    hCdt1(1-100)ΔCy-mCherry-P2A-mVenus-hGeminin(1-110) (CDT1, GMNN Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    human Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…
    Promoter
    EF1a
    Available since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193139-plasmid-map-sequence-id-380234

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  29. TMPRSS2 g2

    Plasmid
    #153016
    Purpose
    A guide RNA targeting TMPRSS2 in a lentiviral plasmid co-expressing mCherry
    Depositor
    Jason Sheltzer
    Insert
    TMPRSS2 gRNA
    Use
    CRISPR and Lentiviral
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    Available since
    June 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    153016-plasmid-map-sequence-id-298699

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  30. TMPRSS2 g3

    Plasmid
    #153017
    Purpose
    A guide RNA targeting TMPRSS2 in a lentiviral plasmid co-expressing mCherry
    Depositor
    Jason Sheltzer
    Insert
    TMPRSS2 gRNA
    Use
    CRISPR and Lentiviral
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    Available since
    June 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    153017-plasmid-map-sequence-id-298697

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  33. pUltra-hot

    Plasmid
    #24130
    Purpose
    3rd generation Lentiviral vector for bi-cistronic expression of mCherry and the gene of interest
    Depositor
    Malcolm Moore
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 26, 2010
    Availability
    Academic Institutions and Nonprofits only
    24130-plasmid-map-sequence-id-247629

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  34. ACE2 g2

    Plasmid
    #153012
    Purpose
    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing mCherry
    Depositor
    Jason Sheltzer
    Insert
    ACE2 gRNA (ACE2 Human)
    Use
    CRISPR and Lentiviral
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    Available since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    153012-plasmid-map-sequence-id-298678

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  35. ACE2 g3

    Plasmid
    #153013
    Purpose
    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing mCherry
    Depositor
    Jason Sheltzer
    Insert
    ACE2 (ACE2 Human)
    Use
    CRISPR and Lentiviral
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    Available since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    153013-plasmid-map-sequence-id-298679

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  36. FUW-TIE1-Cherry

    Plasmid
    #119236
    Purpose
    Plasmid encodes full-length human Tie1 gene fused in-frame with Cherry cloned in lentiviral vector.
    Depositor
    Kari Alitalo
    Insert
    TIE1-mCherry (TIE1 Human)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    P hUbC
    Available since
    Dec. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    119236-plasmid-map-sequence-id-231690

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  38. pMCB320

    Plasmid
    #89359
    Purpose
    3rd generation lentiviral sgRNA expression vector with mCherry, puromycin resistance, BlpI+BstXI cloning sites
    Depositor
    Michael Bassik
    Article
    Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103
    Insert
    puromycin resistance, mCherry
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    89359-plasmid-map-sequence-id-176457

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  39. pUltra-hot-H2B-mTagBFP2

    Plasmid
    #161792
    Purpose
    3rd generation lentiviral multicistronic vector for mammalian expression of mCherry, H2B-mTagBFP2, and one gene of interest
    Depositor
    Jorge Luis Arias-Arias
    Insert
    H2B-mTagBFP2
    Use
    Lentiviral; Multicistronic
    Tags
    mCherry and mTagBFP2
    Expression
    Mammalian
    Mutation
    I174A in the mTagBFP2 protein according to PLoS O…
    Promoter
    UbC
    Available since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    161792-plasmid-map-sequence-id-316815

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  40. LentiGuide Cherry

    Plasmid
    #170510
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and mCherry marker from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Pavel Tolar
    Article
    Newman et al biorXiv 2021.03.02.433398
    Inserts
    S. pyogenes sgRNA cassette
    mCherry
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a and hU6
    Available since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    170510-plasmid-map-sequence-id-343694

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Showing: 721 - 760 of 1147 results