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Plasmid#218862PurposeGal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertRSET-jGCaMP8m
UseTags6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…ExpressionInsectMutationA25G F286YPromoterhsp70Available sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
VV229: GCaMP6f in fck
Plasmid#58514PurposeExpresses GCaMP6f under the a-CamKII promoterDepositorInsertGCaMP6f
UseLentiviralTagsC-terminal FCYENEV (ER export motif); this was no…ExpressionMammalianMutationPromotera-CamKIIAvailable sinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
vGAT-pH pcaggs SE
Plasmid#78578Purposeimage recycling of VGAT in live neuronsDepositorInsertvGAT-pHluorin C-term (Slc32a1 Rat)
UseTagssuperecliptic pHluorinExpressionMammalianMutationPromoterchicken actinAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
msfGFP-Cx43
Plasmid#69024PurposeExpresses rat Cx43 (Gja1 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to msfGFP (FP fused to NT of connexin). CMV promoter.DepositorInsertCx43 (Gja1 Rat)
UseTagssfGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1-14-3-3 gamma GST
Plasmid#13280DepositorInsert14-3-3 gamma (Ywhag Rat)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-Axon-jGCaMP8s
Plasmid#172720PurposeAAV transfer plasmid for neuronal expression of axon-targeted jGCaMP8s.DepositorInsertAxon-jGCaMP8s
UseAAVTags6xHisExpressionMammalianMutationPromoterhSynAvailable sinceDec. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pN1-RnKIF5C(1-560)-Halo-Flag
Plasmid#188071PurposeMammalian cell expression of truncated version (aa 1-560) of rat kinesin-1 KIF5C tagged with Halo and Flag tagsDepositorInsertrat KIF5C (Kif5c Rat)
UseTagsFlag and HaloExpressionMammalianMutationaa1-560PromoterCMVAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
Stx1a-12aa-mCer
Plasmid#53236PurposeFluorescent probe: Syntaxin 1A, C-terminally-tagged with monomeric Cerulean, connected with a 12 amino acid linker. Expressed in mammalian cells, used with SNAP25B-12aa-mCit to measure FRET & dispers.DepositorInsertSyntaxin 1A (Stx1a Rat)
UseTags12aa-linker and mCeruleanExpressionMammalianMutationPromoterAvailable sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-OMOR-eYFP
Plasmid#66847PurposeExpresses Opto-MOR in a Cre dependent mannerDepositorInsertOpto Mu opioid receptor
UseAAV and Cre/LoxTagseYFPExpressionMammalianMutationPromoterEf1aAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBluescript-Evf1
Plasmid#99479PurposeSubcloning of the Evf1 long non-coding RNADepositorInsertEvf1
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA 3.1 Flag-rat-PTP1B
Plasmid#35988DepositorInsertprotein tyrosine phosphatase 1B (Ptpn1 Rat)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJune 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
VAMP2-pHuji
Plasmid#105289PurposeVAMP2 tagged with pH-sensitive red fluorescent protein pHujiDepositorInsertVAMP2 (Vamp2 Rat)
UseTagspHujiExpressionMammalianMutationPromoterAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-APOBEC1-YTHmut
Plasmid#200159PurposeFor bacterial expression of the APOBEC1-YTHmut fusion protein for protein purificationDepositorInsertAPOBEC1-YTHmut
UseTags6xHis, HA, and MBPExpressionBacterialMutationCodon optimized for E coli expressionPromoterLacZAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-H2BmGreenLantern
Plasmid#177332PurposeTo express a bright monomeric green FP to label eucaryotic cell nuclei. To be used in clearing and other fluorescent microscope methods.DepositorInsertH2B (Hist1h2bq Rat)
UseAAVTagsmGreenLanternExpressionMammalianMutationThis fusion protein was optimized to the Human co…PromoterCAGAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFsynW KDEL Hook
Plasmid#197277PurposeEncodes ER-targeted streptavidin-KDEL hook (RUSH system).DepositorInsertStreptavidin
UseLentiviralTagsFLAGExpressionMutationPromoterhSyn (human synapsin I promoter)Available sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pBAC-ie1-DsRed-ObirOrco-QF2-15xQUAS-GCaMP6s
Plasmid#200400PurposeExpresses DsRed broadly, expresses QF2 and GCaMP6s in OSNs of the clonal raider antDepositorInsertsDiscosoma red fluorescent protein
Q factor 2
GCaMP6s
UseTagsExpressionInsectMutationPromoter15x QUAS, ObirOrco (Oocearaea biroi Orco promoter…Available sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO_EGFP-GFE3
Plasmid#79871PurposeFor generating adeno-associated virus to express GFE3 under the EF1a promoter in a 'Cre-on' dependent manner.DepositorInsertGFP-GFE3
UseAAV, Cre/Lox, and Synthetic BiologyTagsGFP and HAExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-SypHluorin 4x (S4x)
Plasmid#37005DepositorInsertSypHluorin 4x (Syp Rat)
UseTagsHAExpressionMammalianMutationFour copies of pHluorin inserted at site 184-185PromoterCMVAvailable sinceJuly 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFSynW SV2A HaloTag
Plasmid#218545PurposeLentiviral expression of SV2A HaloTagDepositorInsertSV2A
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only