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Plasmid#80058PurposeMCS for cloning promoter to drive HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
UseTags3x HAExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pT2KXIGdeltaIn-MCS-birA-tagRFP
Plasmid#58378Purposetol2 transgenesis for biotin based TRAP - BirA ligaseDepositorInsertbirA tagRFP
UseTagstagRFPExpressionMutationPromoterAvailable sinceAug. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMT-myl7-cytoBirA-2A-mCherry_Ras
Plasmid#80060PurposeMyl7 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialMutationPromoterMyl7 promoterAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT-ubb-cytoBirA-2a-mCherry
Plasmid#79885PurposeUbiquitin promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
UseTags3x HAExpressionBacterialMutationPromoterUbiquitinAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS-foxc1a-2a-nlstdTomato
Plasmid#132970Purposefull-length of foxc1a in frame with viral 2a peptide and nuclear localized tdTomato in pCS expression vectorDepositorInsertsUseTagsNLS and mycExpressionMammalianMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSYC-181
Plasmid#31566DepositorInsertpCS4+-P2A
UseTagsExpressionMutationPromoterAvailable sinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pGEM-T Easy-Gbait-hs-QF2
Plasmid#122563PurposeIntegration of QF2 sequence using the CRISPR/Cas9 system.DepositorInsertGbait-hsp70-QF2-SV40pA
UseCloningTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Fuse-gcry-mRFP
Plasmid#117784PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertmRFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
p3E-rab5aa
Plasmid#109716PurposeMultisite gateway vector for 3' tagging with rab5aaDepositorInsertrab5aa (rab5aa Zebrafish)
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEM-cytoBirA-2A-mCherry-SV40pA-FKF
Plasmid#79887PurposeDonor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertHA-tagged BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pME-Trpa1b-2A-mCherry
Plasmid#106291PurposeGateway middle entry construct containing zebrafish Trpa1b, 2A self-cleaving peptide and mCherryDepositorInsertTrpa1b (trpa1b Zebrafish)
UseGateway middle entryTags2A-mCherryExpressionMutationPromoterAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDest-Tol2 (Chien)-Krt4:Gal4VP16:tdTomato:5xUAS_MCS
Plasmid#105649PurposetdTomato expression in differentiated keratinocytes of the periderm layer and during early larval stages also in the basal layer of the epidermisDepositorInsertKrt4:Gal4VP16:tdTomato:5xUAS_MCS
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
Cs2+Etv4(pea3)
Plasmid#49093Purposeexpression of Etv4DepositorInsertEtv4 (etv4 Zebrafish)
UseXenopus/avian/zebrafishTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
SI14-Kit b
Plasmid#46304DepositorInsertKit receptor b mouse monoclonal antibody (kitb Zebrafish)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-rab2a
Plasmid#109619PurposeMuscle specific zebrafish expression of mKate2 fused to rab2aDepositorInsertrab2a (rab2a Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pPRISM-Cre-cmlc2-eGFP
Plasmid#117794PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertCre
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
CACNA1F_L (OZ507)
Plasmid#26957DepositorInsertZinc finger array targeting cacna1f (cacna1f Zebrafish)
UseZebrafish targetingTagsExpressionMutationPromoterAvailable sinceJan. 25, 2011AvailabilityAcademic Institutions and Nonprofits only