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Plasmid#102334PurposeExpresses WT ScVrg4 in S. cerevisiae cells using Galactose induction, -URA and -Leu selectionDepositorInsertYGL225W (VRG4 Budding Yeast)
UseTagsGFPExpressionYeastMutationPromoterGAL1Available sinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-FLAG-SENP3-C526A
Plasmid#115522PurposeMammalian expression constructDepositorInsertSENP3-C526A (Senp3 Mouse)
UseTagsFLAGExpressionMammalianMutationC526APromoterAvailable sinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUASp-Egl-Trbo
Plasmid#179993PurposeExpresses Egl fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system.DepositorInsertEgl-Trbo
UseTagsTurboIDExpressionInsectMutationPromoterUAS-Gal4Available sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMLS714
Plasmid#188886PurposeMiniMos Transposon vector carrying Pmex-5::2xNLS::Cas9 and floxed Cbr-Unc-119DepositorInsertPmex-5::Cas9
UseTagsExpressionWormMutationC. elegans codom optimization and intron additionPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-mGinger1
Plasmid#119784PurposeExpresses mGinger1 tagged N-terminally with an MHHHHHHGSG tagDepositorInsertmGinger1
UseTags6xHis tagExpressionBacterialMutationPromoterT7Available sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-30nmSAH-GFP(1-10)
Plasmid#61677PurposeGFP-labeled SAH scaffold with split GFP attachment siteDepositorInsertmEGFP-30nmSAH-GFP(1-10)
UseTagsExpressionMutationPromoterAvailable sinceMarch 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-BsmBI-HA
Plasmid#162802PurposeSTELLA destination plasmid with HA terminal tag; Suitable for amber suppression (4xMmaPylT cassette); for transient expressionDepositorInsertUb-BsmBI-HA
UsePiggybacTagsHAExpressionMammalianMutationPromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
hisSUMO-mZFP57
Plasmid#87224PurposeExpress hisSUMO tagged Zinc finger protein 57 F1-F3(mouse) in E. coliDepositorInsertZFP57 (Zfp57 Mouse)
UseTagshisSUMOExpressionBacterialMutationPromoterT7Available sinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-BsmBI
Plasmid#162801PurposeSTELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient expressionDepositorInsertUb-BsmBI
UsePiggybacTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT7-stl
Plasmid#51721Purposeconditional gene knockdowns in Trypanosoma bruceiDepositorTypeEmpty backboneUseConditional gene knockdown in t. bruceiTagsExpressionMutationPromoterT7 (2X)Available sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only