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Plasmid#197950PurposeT-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter, with SV40 NLS at the C-terminal.DepositorInsertpcoCasphi-2
UseCRISPRTagsExpressionPlantMutationPromoterpUBQ10Available sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
epiCRISPRn
Plasmid#135961PurposeExpresses SpCas9, and cloning backbone for sgRNADepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceApril 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLSLZ.D.R
Plasmid#51510PurposeSingle component LSL vector. Contains 2x35S:Zif268:FokI followed by the us:NPTII template. This sequence is between the LIR and SIR.DepositorInsertsZif268:FokI
us:NPTII repair template
UsePlant t-dna plasmidTagsNLSExpressionMutationPromoter2x35SAvailable sinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
KA2058_pX330A-G2P
Plasmid#124202PurposeExpression of SpCas9 and sgRNADepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFTK056
Plasmid#171328PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertSpCas9-NLS
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
px459 EQR
Plasmid#101732PurposeExpresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifsDepositorInsertSpCas9 EQR
UseTagsExpressionMammalianMutationVQRPromoterAvailable sinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pICSL1P3_AtU626Ter26
Plasmid#191771PurposeCas9 guide expression in dicotsDepositorInsertSpCas9 guide scaffold with AtU626 promoter/terminator
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJB057
Plasmid#98911PurposeExpress Dronpa tagged ZapA in E coliDepositorInsertZapA
UseTagsDronpaExpressionBacterialMutationPromoterT5-lacAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
SPDK3214
Plasmid#97406Purposebinary construct of ER-targeted GUS with sfCherry11 tag for plant expressionDepositorInsertER-GUS-sfCherry11
UseTagsER targetExpressionPlantMutationPromoterAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRANS_232
Plasmid#91121PurposeTransformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: ToLCVDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoter35SAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMPO 58
Plasmid#68362PurposeExpression vectors for the salycilate cascade expression system (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm- nasF attenuator-T7 SD sequence-MCSII, ColE1 replication originDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
p202_LTJ_sgRNACD45.2_R1
Plasmid#82672PurposesgRNA targeting murine CD45.2 region 1. Co-expresses SpCas9-2A-EGFP.DepositorInsertsgRNA targeting mouse CD45.2 region 1
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTX168
Plasmid#89257PurposeExpress STU (Single Transcriptional Unit) Cas9 with CaMV 35s promoter in plantsDepositorTypeEmpty backboneUseCRISPRTagsSV40 NLSExpressionPlantMutationPromoterAvailable sinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGJJ055
Plasmid#183622PurposeddPCA mutagenesis plasmid - Efficient mutant library cloning plasmid suitable for oligo synthesis, error prone PCR or nicking mutagenesis strategies.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
Guide-Generating Vector
Plasmid#125752PurposeGuide-Generating Vector for creating the Cys4-guide-scaffold structure in the first round of assemblyDepositorInsertCys4-GFPdropout-SpCas9scaffold
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
CAG::PSAMQ79G,Q139G:5HT3HC-IRES-GFP
Plasmid#32474DepositorInsertPSAMQ79G,Q139G:5HT3 HC
UseTagsIRES-EGFPExpressionMammalianMutationPromoterCAGAvailable sinceApril 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pZLSLD.R
Plasmid#51511PurposeSingle component LSL vector. Contains 2x35S:Zif268:FokI outside of the replicon borders. Within the replicon is the us:NPTII template.DepositorInsertsZif268:FokI
us:NPTII repair template
UsePlant t-dna plasmidTagsNLSExpressionMutationPromoter2x35SAvailable sinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
AL12R-EF-Tu
Plasmid#161013PurposePP7-tagged reporter driven by the Arabidopsis EF-Tu promoterDepositorInsertPP7 and H2B-mScarlet
UseTagsExpressionPlantMutationPromoterAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFTK063
Plasmid#171335PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertdSpCas9(m4) (for fusion)
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK062
Plasmid#171334PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertdSpCas9(m2) (for fusion)
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only