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Plasmid#223213PurposeLentiviral vector - mCherry reporter and TGFb1 for Gal4DBDVP64 synNotch receptors with a constitutive BFPDepositorInsertTGFb1 active - membrane (TGFB1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-LexAOx4-Pmin-Fluc-PGK-Rluc-P2A-mCherry
Plasmid#192634PurposeLentiviral vector for inducible Fluc expression with 4x LexA operator (LexAO) and constitutive Rluc and mCherry.DepositorInsertfirefly luciferase
UseLentiviralTagsExpressionMammalianMutationPromoterMinimal promoterAvailable sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR- GAL4-UAS-BAMH1-IRES-mCherry-PGK-BFP
Plasmid#223215PurposeLentiviral vector - mCherry reporter with a BAMH1 site for Gal4DBDVP64 synNotch receptors with a constitutive BFPDepositorInsertmCherry BFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV TRE3G Puro FLAG-DD-ER-mCherry-TRF1-FokI WT
Plasmid#207832Purposetelomere specific nuclease to create double-strand breaks at telomeresDepositorInsertTRF1 (TERF1 Human)
UseLentiviralTagsFlag, DD, ER, mCherry and FokIExpressionMammalianMutationPromoterTRE3GAvailable sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPL7552_ColE1_PiggyBac_2xcHS4::mCherry-IRES-PuroR-P2A-mTagBFP2-NLS::2xcHS4
Plasmid#217971PurposeProvides expression from a CMV promoter surrounded by insulators. Integrates via piggyBac transposase. Confers puromycin resistance. Expresses nuclear localized BFP. High copy plasmid.DepositorInsert2xcHS4::mCherry-IRES-PuroR-mTagBFP2-NLS::2xcHS4
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3- sgAAVS1.5-CAG-Cas9-T2A-mCherry-P2A-Puro
Plasmid#216246PurposeExpress Cas9 with CAG promoter to improve the expression in hES cells and a highly efficient sgAAVS1.5 for AAVS1 integrationDepositorInsertCas9 and sgAAVS1.5
UseCRISPR; Human safe harbor locus (a avs1) site-spe…TagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR_UCOE-EF1a-dCas9-XTEN80-KRAB(Kox1)-IRES-mCherry
Plasmid#188769PurposedCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)DepositorInsertdCas9-XTEN80-KRAB(Kox1)
UseLentiviralTagsHA-2xNLS-XTEN80(linker)-KRAB(Kox1)ExpressionMutationPromoterEF1aAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-aCamkII-mCherry-P2A-Cre-WPRE-BGH-polyA
Plasmid#107313PurposeEncoding aCamkII-mCherry-CreDepositorInsertaCamkII-mCherry-P2A-Cre-WPRE-BGH-polyA
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNQL004-SOX2-FKBPV-HA2-P2A-mCherry targeting construct
Plasmid#175552PurposeTargeting vector to introduce an FKBPV-HA2-P2A-mCherry cassette at the mouse SOX2 locus. Degradation Tag (dTAG) system.DepositorInsertSOX2 (Sox2 Mouse)
UseMouse TargetingTagsFKBPV-HA2-P2A-mCherryExpressionMammalianMutationnonePromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3-PAR1-GFP11-T2A-GFP10-bArrestin1-T2A mCherry
Plasmid#113608PurposePPI reporterDepositorInsertPAR1
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E4orf6
Plasmid#64222PurposeExpression vector for sgRNA and for Expression of Cas9 linked via T2A to mCherry linked to the Ad4 E4orf6 gene via P2ADepositorInsertsCas9
sgRNA cassette
UseCRISPRTags3xFLAG, NLS, and T2A-mCherry-P2A-E4orf6ExpressionMammalianMutationPromoterCBh and U6Available sinceMay 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO {hCAR}off-{hM4Di-mCherry}on-W3SL
Plasmid#111397PurposeAAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON hM4Di-mCherry (Gi-coupled DREADD for neuronal silencing), W3SL cassette (for maximize cloning capacity)DepositorInsertsUseAAVTagsMyc and mCherryExpressionMammalianMutationPromoterhSynapsinAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
vYM073_PB-pEF-osTIR1-T2A-mCherry-AID-Flag-BlastR
Plasmid#160042PurposeFor the auxin receiver that report on auxin.DepositorInsertsosTIR1
BlastR
UseLuciferaseTagsmAID and mCherryExpressionMammalianMutationPromoterAvailable sinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJT371 PB ITR and Cp36 attD pEF Puro-2a-mCherry donor
Plasmid#184951PurposeDonor for both PiggyBac and Cp36 RecombinaseDepositorInsertPuroR, P2A, mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-Flex/3'USS-mCherry(ATG full mut)
Plasmid#197893PurposeCan be used to generate AAV virus that will express mCherry in the presence of Cre and the leakage expression is significantly reduced without CreDepositorInsertmCherry
UseAAVTagsExpressionMutationN/APromoterEF1aAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E1B
Plasmid#64221PurposeExpression vector for sgRNA and for Expression of Cas9 linked via T2A to mCherry and to Ad4 E1B55K via P2ADepositorInsertsCas9
sgRNA cassette
UseCRISPRTags3xFLAG, NLS, and T2A-mCherry-P2A-E1BExpressionMammalianMutationPromoterCBh and U6Available sinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMB953:PB-CAG-PuroR-nGFP-FLEx-mCherry-KASH-WPRE
Plasmid#168106PurposePiggyBac transposon vector constitutively driving PuroR-P2A-eGFP and Cre conditional expression of KASH nuclear membrane-bound mCherry. Conditional insert flanked by MCS for cloningDepositorInsertmCherry-KASH
UseCRISPR and Cre/LoxTagsExpressionMutationPromoterCAGAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-sgRANGAP.C1-CAG-Cas9-T2A-mCherry-P2A-Puro
Plasmid#216249PurposeExpress Cas9 with CAG promoter to improve the expression in hES cells and sgRANGAP.C1 to tag endogenous RANGAP1 C-terminusDepositorInsertCas9 and sgRANGAP.C1 (RANGAP1 Human)
UseCRISPR; Endogenous taggingTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTol2p2A--3mnx1:loxP-mTagBFP2-stop-loxP-mCherry-NS-MCS
Plasmid#78251PurposeCan be used to subclone in tagged gene of interest into inducible motor neuron expression vector.DepositorInsertsUseZebrafish motor neuron inducible expressionTagsExpressionMutationPromoter-3mnx1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3-muOR-GFP11-T2A-GFP10-bArrestin1-T2A mCherry
Plasmid#113611PurposePPI reporterDepositorInsertmu opioid receptor
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only