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Plasmid#89888PurposeHuman KRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.DepositorInsertmCherry-KRasG12V
UseUnspecifiedTagsmCherryExpressionMutationPromoterAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
CAG-Crx-IRES-GFPd2
Plasmid#73997PurposePlasmid expressing Crx-IRES- GFPd2DepositorInsertCrx-IRES-GFPd2 (Crx Mouse)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCIneoLuc-PP2A
Plasmid#128349PurposeVector for LUMIER assayDepositorInsertPP2A (PTPA Human)
UseTagsLuciferaseExpressionMammalianMutationPromoterCMVAvailable sinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCrysβ:ECFP-LexOP:mCherry-HrasG12V
Plasmid#89887PurposeHuman HRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.DepositorInsertmCherry-HRasG12V
UseUnspecifiedTagsmCherryExpressionMutationPromoterAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBW2286_pCAG-PV1-FlpO-N396-L1-ABI-NLS-BGHpA
Plasmid#108769PurposeExpresses N-terminus to aa396 of Flp recombinase fused to ABI CIDDepositorInsertFlp recombinase fragment N-terminus to aa396
UseCre/Lox and Synthetic BiologyTagsABI,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mEGFP-CaMKIIa (T305D/T306D)
Plasmid#127611PurposeFluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T305D/T306D)DepositorInsertcalcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
UseTagsmEGFPExpressionMammalianMutationchanged Threonine 305/306 to AlaninePromoterpCAGAvailable sinceJan. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST-DHFR F[3]-N (LEU2)
Plasmid#177798PurposeGateway destination vector to insert genes of interest having a N-terminal DHFR F[3] fusion for DHFR-PCA/BFG-PCA assays.DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterADH1Available sinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEMS2021
Plasmid#49128PurposeAAV plasmid with DCX (Ple302) promoter driving expression of iCre.DepositorInsertssAAV-Ple302-iCre
UseAAVTagsExpressionMammalianMutationPromoterDCXAvailable sinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTol2CG2-hsp70I:QFGal4-SV40pA
Plasmid#155120PurposeTol2 vector for heat-shock inducible expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis markerDepositorInserthsp70l:QFGal4 (hsp70l Neurospora crassa, Budding Yeast, Zebrafish)
UseZebrafish expressionTagsExpressionMutationPromoterhsp70lAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pExpreS2-1-CR-PA-10H-SIII
Plasmid#175447PurposeHiFi-compatible destination vector for secreted overexpression of thrombin-cleavable, C-terminal Protein A-10His-TwinStrep tagged proteins with ExpreS2 PlatformDepositorTypeEmpty backboneUseTagsBiP Secretion Peptide and Thrombin-Protein A-10Hi…ExpressionInsectMutationPromoterFused Actin-HSP70Available sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEMS1977
Plasmid#49109PurposeAAV plasmid with C8ORF46 (Ple251) promoter driving expression of iCre.DepositorInsertssAAV-Ple251-icre
UseAAVTagsExpressionMammalianMutationPromoterC8ORF46Available sinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEMS2157
Plasmid#70118PurposePromoterless AAV plasmid with MCS to insert promoter for expression of EmGFP. Contains WPRE.DepositorInsertssAAV-MCS-EmGFP-WPRE
UseAAVTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceMay 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
Plxna2-AP-His
Plasmid#71997PurposeExpresses the extracellular region of the PlexinA2 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertPlxna2 (Plxna2 Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Myc-huMEKK3 (K391A)
Plasmid#44157DepositorInsertMEKK3 (K391A) (MAP3K3 Human)
UseTagsMycExpressionMammalianMutationK391A, kinase-dead mutantPromoterAvailable sinceApril 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
eGFP-KCC2
Plasmid#104075Purposeexpresses rat KCC2 (b isoform) with eGFP tag at the N-terminus of KCC2DepositorInsertKCC2 (b isoform) (Slc12a5 Rat)
UseTagsEGFPExpressionMammalianMutationEcoR1 site in rat KCC2 was removed by silent muta…PromoterCMVAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-Braf R188L
Plasmid#118330PurposeExpression of human BrafDepositorInsertBraf R188L (BRAF Human)
UseTagsGFPExpressionMammalianMutationR188LPromoterCMVAvailable sinceNov. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pClneoMyc MOB1 T12/35A
Plasmid#47938Purposeexpresses Myc tagged human MOB1 containing T12A and T35A mutationsDepositorInsertMOB1A (MOB1A Human)
UseTagsMycExpressionMammalianMutationT12A and T35APromoterCMVAvailable sinceSept. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
STK16_HUMAN_D0
Plasmid#79701PurposeThis plasmid encodes the kinase domain of STK16. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertSTK16 (STK16 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEMS2275
Plasmid#111893PurposeHigh Copy number plasmid containing Ple345 (NEFL MiniPromoter) insert.DepositorInsertPle345 (NEFL Human)
UseUnspecifiedTagsExpressionMutationPromoterPle345 (NEFL MiniPromoter)Available sinceAug. 10, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
Dscam1_7.27.30 EC10-Fc-His
Plasmid#72190PurposeExpresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.30 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertDscam1_7.27.30
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only