Skip to main content
Addgene
Showing: 3761 - 3780 of 20429 results

  1. pTL-Bag1-gRNA1

    Plasmid
    #196251
    Purpose
    Plasmid for cloning the first CRISPR-Cas9 guide RNA of multiplex guide RNAs
    Depositor
    Bing Yang
    Article
    Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1
    Insert
    CRISPR-Cas9 guide RNA scaffold and multiple cloning site
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119 promoter
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196251-plasmid-map-sequence-id-385941

    Enlarge

  2. pTL-Bag4-gRNA4

    Plasmid
    #196255
    Purpose
    Plasmid for cloning the 4th CRISPR-Cas9 guide RNA of 4 guide RNAs
    Depositor
    Bing Yang
    Article
    Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1
    Insert
    CRISPR-Cas9 guide RNA scaffold and multiple cloning site
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119 promoter
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196255-plasmid-map-sequence-id-385931

    Enlarge

  5. dpYPQ230-D156R-CBE2

    Plasmid
    #195368
    Purpose
    dLbCas12a-D156R based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-D156R with the linker of XTEN
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1
    Insert
    hA3A/Y130F-XTEN-dLbCas12a-D156R-UGI
    Use
    CRISPR; Gateway compatible ha3a/y130f-xten-dlbcas…
    Tags
    Expression
    Plant
    Mutation
    Promoter
    ZmUBI1
    Available since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    195368-plasmid-map-sequence-id-392913

    Enlarge

  7. FUG-T2A-Cas9

    Plasmid
    #75346
    Purpose
    Ubiquitin promoter expresses GFP and humanized spCas9 (from PX330) via a T2A motif. For cell transfection or use in lentiviral packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.
    Depositor
    Bryan Luikart
    Article
    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10
    Insert
    Cas9
    Use
    CRISPR and Lentiviral
    Tags
    GFP (via t2a)
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    May 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    75346-plasmid-map-sequence-id-142581

    Enlarge

  8. MSP2372

    Plasmid
    #73196
    Purpose
    Human expression plasmid for SpCas9(R661A/Q695A/Q926A) variant: CMV-T7-humanSpCas9(R661A, Q695A, Q926A)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (R661A/Q695A/Q926A)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    R661A, Q695A, and Q926A in SpCas9
    Promoter
    CMV
    Available since
    Feb. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    73196-plasmid-map-sequence-id-134659

    Enlarge

  14. pUb-Cas9-mCherry_cU6:6-sgRNA

    Plasmid
    #190598
    Purpose
    The plasmid encodes S. pyogenes Cas9 and mCherry separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter
    Depositor
    Claudia Rueckert
    Article
    Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.
    Inserts
    Cas9
    sgRNA
    Use
    CRISPR
    Tags
    mCherry - separated by T2A
    Expression
    Mutation
    Promoter
    Aedes aegypti polyubiquitin and Culex quinquefasc…
    Available since
    Jan. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    190598-plasmid-map-sequence-id-379953

    Enlarge

Showing: 3761 - 3780 of 20429 results