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pNLF1-C_CDC40:1-579

Plasmid

#211065

Purpose

Mammalian expression of full-length human CDC40. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

CDC40:M1-D579 (CDC40 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_RBBP4:1-425

Plasmid

#211019

Purpose

Mammalian expression of full-length human RBBP4. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

RBBP4:M1-S425 (RBBP4 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_WDR5:1-334

Plasmid

#211026

Purpose

Mammalian expression of full-length human WDR5. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

WDR5:M1-C334 (WDR5 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_DCAF1:1038-1400

Plasmid

#210963

Purpose

Mammalian expression of human DCAF1 fragment E1038 to E1400. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

DCAF1:E1038-E1400 (DCAF1 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_CORO1A:1-461

Plasmid

#210965

Purpose

Mammalian expression of full-length human CORO1A. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

CORO1A:M1-K461 (CORO1A Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_FBXW11:1-542

Plasmid

#210952

Purpose

Mammalian expression of full-length human FBXW11. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

FBXW11:M1-R542 (FBXW11 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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EV-D68 3C protease; aliases: Enterovirus A71 3C protease, non cleavable C-term 6xHis tag

Plasmid

#204817

Purpose

Bacterial expresison of Enterovirus D68 3C protease

Depositor

Frank von Delft, ASAP Discovery

Insert

EV D68 3C protease

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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EV-A71 3C protease; aliases: Enterovirus A71 3C protease, non cleavable C-term 6xHis tag

Plasmid

#204816

Purpose

Bacterial expresison of Enterovirus A71 3C protease

Depositor

Frank von Delft, ASAP Discovery

Insert

EV A71 3C protease

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 17, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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CAG-LoxpStopLoxp-NLS-dCas9-HA-NLS-NLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-T2A-EGFP-WPRE-PolyA

Plasmid

#107307

Purpose

Encoding an SPH activation system

Depositor

Hui Yang

Article

Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/

Insert

CAG-LSL-dCas9-10xGCN4-P2A-scFv-P65-HSF1-T2A-EGFP-WPRE-PolyA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pET30(a)-AtNUDX23

Plasmid

#175021

Purpose

Purification of AtNUDX23 enzyme

Depositor

Jeppe Vinther

Article

Sherwood et al Nature. 2023 Jul;619(7971):811-818. doi:

Insert

AtNUDX23 (NUDX23 Mustard Weed)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-MPP8-10xHis

Plasmid

#194178

Purpose

Expresses MPP8-10xHis tagged

Depositor

Matthias Leisegang, Ralf Brandes

Article

Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

Insert

MPHOSPH8 (MPHOSPH8 Human)

Use

Tags

10xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-hCCND1-HA

Plasmid

#174154

Purpose

Lentiviral vector expressing HA-tagged human cyclin D1

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

CCND1 (CCND1 Human)

Use

Lentiviral

Tags

hemagglutinin (HA)

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer10b-HA-KRAS WT CO SR

Plasmid

#164926

Purpose

Expresses HA tagged, codon Optimized and siRNA-resistant, tetracycline-inducible KRAS wild type sequence

Depositor

Ji Luo

Article

Yuan et al Cancer Discov. 2014 Oct;4(10):1182-1197.

Insert

KRAS proto-oncogene, GTPase (KRAS) (KRAS Human)

Use

Lentiviral

Tags

HA tag

Expression

Mutation

9 Valine codons are optimized and siRNA targetin…

Promoter

Ubc promoter

Available since

May 27, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer10b-HA-KRAS G12V CO SR

Plasmid

#164928

Purpose

Expresses HA tagged, codon Optimized and siRNA-resistant, tetracycline-inducible KRASG12V sequence

Depositor

Ji Luo

Article

Yuan et al Cancer Discov. 2014 Oct;4(10):1182-1197.

Insert

KRAS proto-oncogene, GTPase (KRAS) (KRAS Human)

Use

Lentiviral

Tags

HA tag

Expression

Mutation

Glycine 12 changed to valine; 9 Valine codons are…

Promoter

Ubc promoter

Available since

May 27, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-hCCND1-T2A-hCCND2-P2A-hCCND3

Plasmid

#174156

Purpose

Lentiviral vector expressing all three D-type cyclins (cyclin D1, cyclin D2, cyclin D3) from a single promoter

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

CCND1>T2A>CCND2>P2A>CCND3

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2_AMBRA1#2

Plasmid

#174153

Purpose

Lentiviral vector expressing Cas9 and a sgRNA against the human AMBRA1 gene

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

AMBRA (AMBRA1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2_AMBRA1#1

Plasmid

#174152

Purpose

Lentiviral vector expressing Cas9 and a sgRNA against the human AMBRA1 gene

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

AMBRA (AMBRA1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pPB-AMBRA1-3xFLAG

Plasmid

#174159

Purpose

PiggyBac vector to stably express 3xFLAG-tagged AMBRA1

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

AMBRA1 (AMBRA1 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV6-MPP8-W80A-10xHis

Plasmid

#194179

Purpose

Expresses MPP8-W80A_10xHis

Depositor

Matthias Leisegang, Ralf Brandes

Article

Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

Insert

MPHOSPH8 (MPHOSPH8 Human)

Use

Tags

10xHis

Expression

Mammalian

Mutation

MPP8-W80A inside chromo domain

Promoter

CMV

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-MPP8-delta2-441-10xHis

Plasmid

#194181

Purpose

Expresses MPP8-delta2-441_10xHis

Depositor

Matthias Leisegang, Ralf Brandes

Article

Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

Insert

MPHOSPH8 (MPHOSPH8 Human)

Use

Tags

10xHis

Expression

Mammalian

Mutation

MPP8-delta2-441

Promoter

CMV

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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