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Plasmid#218831PurposeExpresses C-terminal Strep-FLAG-tagged M566T human TLK1 variant 5 in a retrovirus vector.DepositorInsertTLK1 M566T (TLK1 Human)
UseTagsFLAG-STREPExpressionMammalianMutationM566TPromoterCMVAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV-CAG-Sun1-GFP-WPRE-pA
Plasmid#220752PurposeExpression of Sun1GFP for nuclei isolationDepositorInsertSun1-GFP-WPRE
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK880
Plasmid#219726PurposeMoClo-compatible Level 0 - promoter vector carrying promoter MinSyn105DepositorInsertpromoter MinSyn105
UseSynthetic BiologyTagsExpressionPlantMutationPromoterMinSyn105Available sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK879
Plasmid#219727PurposeMoClo-compatible Level 0 - promoter vector carrying promoter MinSyn108DepositorInsertpromoter MinSyn108
UseSynthetic BiologyTagsExpressionPlantMutationPromoterMinSyn108Available sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK887
Plasmid#219728PurposeMoClo-compatible Level 0 - promoter vector carrying promoter MinSyn110DepositorInsertpromoter MinSyn110
UseSynthetic BiologyTagsExpressionPlantMutationPromoterMinSyn110Available sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK882
Plasmid#219729PurposeMoClo-compatible Level 0 - promoter vector carrying promoter MinSyn159xDepositorInsertpromoter MinSyn159x
UseSynthetic BiologyTagsExpressionPlantMutationPromoterMinSyn159xAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK881
Plasmid#219730PurposeMoClo-compatible Level 0 - promoter vector carrying promoter MinSyn1556xDepositorInsertpromoter MinSyn1556x
UseSynthetic BiologyTagsExpressionPlantMutationPromoterMinSyn1556xAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK884
Plasmid#219731PurposeMoClo-compatible Level 0 - promoter vector carrying promoter MinSyn1569xDepositorInsertpromoter MinSyn1569x
UseSynthetic BiologyTagsExpressionPlantMutationPromoterMinSyn1569xAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK6030
Plasmid#219732PurposeMoClo-compatible Level 0 - promoter vector carrying promoter MinSyn1637xDepositorInsertpromoter MinSyn1637x
UseSynthetic BiologyTagsExpressionPlantMutationPromoterMinSyn1637xAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK885
Plasmid#219733PurposeMoClo-compatible Level 0 - promoter vector carrying promoter MinSyn1824xDepositorInsertpromoter MinSyn1824x
UseSynthetic BiologyTagsExpressionPlantMutationPromoterMinSyn1824xAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK878
Plasmid#219734PurposeMoClo-compatible Level 0 - promoter vector carrying promoter MinSyn1904xDepositorInsertpromoter MinSyn1904x
UseSynthetic BiologyTagsExpressionPlantMutationPromoterMinSyn1904xAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-H2B-MCS
Plasmid#184041PurposeCloning of GFP-H2B fusion proteinsDepositorTypeEmpty backboneUseTagsGFP and H2BExpressionMammalianMutationPromoterCMVAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-QF2-he1.1:YFP
Plasmid#184819PurposeModified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Backbone into which homology arms are cloned for CRISPR/Cas9-mediated integration of QF2.DepositorArticleInsertQF2-pA-he1.1:YFP
UseCRISPR; Backbone for cloning of homology arms (fo…TagsExpressionMutationPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-QF2-he1.1:YFP-rln3a-HA
Plasmid#184820PurposeModified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Contains homology arm inserts for integration of QF2 into rln3a gene by CRISPR/Cas9 integration.DepositorArticleInsertrln3a-HA
UseCRISPR; For integration of qf2 by homology-direct…TagsExpressionMutationPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
TOPO-nmba
Plasmid#184821PurposeUsed to synthesize RNA probe for in situ hybridization for nmbaDepositorArticleInsertnmba (nmba Zebrafish)
UseTemplate for synthesis of nmba probeTagsExpressionMutationPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-SYN-NLS-SaCas9-NLS-3xHA-bGHpA-U6-BsaI-sgRNA
Plasmid#218710PurposeAAV vector plasmid expressing Cas9 from Staphylococcus aureus (SaCas9) under the human synapsin (SYN) promoter and its sgRNA under the U6 promoterDepositorInsertshSaCas9
Chimeric guide for SaCas9
UseAAV and CRISPRTags3xHA and NLSExpressionMammalianMutationPromoterAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSuperCRISPR-sgRNA-RL69-WT(TTTT)
Plasmid#215847PurposeThis plasmid encodes sgRNA that target Renilla luciferase with stretch sequenceDepositorInsertsgRNA-RL69 with TTTT stretch
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSuperCRISPR-sgRNA-EGFP12-TTTA
Plasmid#215857PurposeThis plasmid encodes sgRNA that target EGFP with stretch sequenceDepositorInsertsgRNA-EGFP12 with TTTA stretch
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSuperCRISPR-sgRNA-RL69-ATTT
Plasmid#215848PurposeThis plasmid encodes sgRNA that target Renilla luciferase with stretch sequenceDepositorInsertsgRNA-RL69 with ATTT stretch
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSuperCRISPR-sgRNA-RL69-CTTT
Plasmid#215852PurposeThis plasmid encodes sgRNA that target Renilla luciferase with stretch sequenceDepositorInsertsgRNA-RL69 with CTTT stretch
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSuperCRISPR-sgRNA-RL69-TTTA
Plasmid#215851PurposeThis plasmid encodes sgRNA that target Renilla luciferase with stretch sequenceDepositorInsertsgRNA-RL69 with TTTA stretch
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSuperCRISPR-sgRNA-RL69-TTAT
Plasmid#215850PurposeThis plasmid encodes sgRNA that target Renilla luciferase with stretch sequenceDepositorInsertsgRNA-RL69 with TTAT stretch
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSuperCRISPR-sgRNA-RL69-TATT
Plasmid#215849PurposeThis plasmid encodes sgRNA that target Renilla luciferase with stretch sequenceDepositorInsertsgRNA-RL69 with TATT stretch
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSuperCRISPR-sgRNA-EGFP12-WT(TTTT)
Plasmid#215856PurposeThis plasmid encodes sgRNA that target EGFP with stretch sequenceDepositorInsertsgRNA-EGFP12 with TTTT stretch
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSuperCRISPR-sgRNA-RL69-TTTC
Plasmid#215855PurposeThis plasmid encodes sgRNA that target Renilla luciferase with stretch sequenceDepositorInsertsgRNA-RL69 with TTTC stretch
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSuperCRISPR-sgRNA-RL69-TCTT
Plasmid#215853PurposeThis plasmid encodes sgRNA that target Renilla luciferase with stretch sequenceDepositorInsertsgRNA-RL69 with TCTT stretch
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSuperCRISPR-sgRNA-RL69-TTCT
Plasmid#215854PurposeThis plasmid encodes sgRNA that target Renilla luciferase with stretch sequenceDepositorInsertsgRNA-RL69 with TTCT stretch
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSAD-dG-F3-H2B-mCherry
Plasmid#206007PurposeG-Deleted Rabies vector expressing H2B-mCherryDepositorInsertH2B-mCherry
UseRabies virusTagsmCherryExpressionMammalianMutationPromoterAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2 RPS28 UTR1 WT
Plasmid#203157PurposeRPS28 UTR luciferase assay plasmid (WT UTR1, sites 1 and 2)DepositorInsertRPS28 UTR (RPS28 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCX:α4-EC4-GA
Plasmid#208514PurposeddGFP-A-inserted Pcdhα4DepositorInsertPcdha4 (Pcdha4 Mouse)
UseTagsHA tagExpressionMammalianMutationPromoterChicken β-actinAvailable sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pCX:cIPAD-3 (full length)
Plasmid#208520PurposeddGFP-A and ddGFP-B3-inserted Pcdhα4DepositorInsertPcdha4 (Pcdha4 Mouse)
UseTagsHA tagExpressionMammalianMutationPromoterChicken β-actinAvailable sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCX:γB2ΔEC1ΔICD-EB
Plasmid#208522PurposeEBFP2-fused PcdhγB2 lacking an EC1 domain and an ICD domainDepositorInsertPcdhgB2 (Pcdhgb2 Mouse)
UseTagsEBFP2ExpressionMammalianMutationPromoterChicken β-actinAvailable sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCX:cIPAD-1 (full length)
Plasmid#208519PurposeddGFP-A and ddGFP-B1-inserted Pcdhα4DepositorInsertPcdha4 (Pcdha4 Mouse)
UseTagsHA tagExpressionMammalianMutationPromoterChicken β-actinAvailable sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCX:γB2ΔEC1ΔICD-mCh
Plasmid#208521PurposemCherry-fused PcdhγB2 lacking an EC1 domain and an ICD domainDepositorInsertPcdhgB2 (Pcdhgb2 Mouse)
UseTagsmCherryExpressionMammalianMutationPromoterChicken β-actinAvailable sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
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LBAPPW
Plasmid#205666PurposeshRNAmir backbone for RNA interference, with Puromycin selectionDepositorInsertmiR-AB
UseLentiviralTagsExpressionMutationPromoterCBHAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
LCAPPW
Plasmid#205648PurposeshRNAmir backbone for RNA interference, with Puromycin selectionDepositorInsertmiR-AB
UseLentiviralTagsExpressionMutationPromoterhCMVAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
LEAPPW
Plasmid#205675PurposeshRNAmir backbone for RNA interference, with Puromycin selectionDepositorInsertmiR-AB
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
LMAPPW
Plasmid#205657PurposeshRNAmir backbone for RNA interference, with Puromycin selectionDepositorInsertmiR-AB
UseLentiviralTagsExpressionMutationPromotermCMVAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-dCer3-C2-FRB
Plasmid#191327PurposeExpresses the fluorescently labeled membrane anchor C2 fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRBDepositorInsertFRB-C2-dCer3
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo-μ3A-(HA)3
Plasmid#198179PurposeExpression of HA-tagged AP-3 μ3A in mammalian cellsDepositorInsertAP-3 μ3A
UseTagstriple HA tagExpressionMammalianMutationsilent substitution in codon 225PromoterCMVAvailable sinceApril 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-NcPAN3_234-656-GW-mut_U
Plasmid#147681PurposeBacterial Expression of NcPAN3_234-656-GW-mutDepositorInsertNcPAN3_234-656-GW-mut
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NcQDE2_506-938-D603K_M
Plasmid#146962PurposeBacterial Expression of NcQde-2_506-938-D603KDepositorInsertNcQde-2_506-938-D603K
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NcQDE2_506-938-K638E_M
Plasmid#146963PurposeBacterial Expression of NcQde-2_506-938-K638EDepositorInsertNcQde-2_506-938-K638E
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NcQDE2 _506-938-A602E_M
Plasmid#146964PurposeBacterial Expression of NcQde-2_506-938-A602EDepositorInsertNcQde-2_506-938-A602E
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NcQDE2 _506-938-H609W_M
Plasmid#146965PurposeBacterial Expression of NcQde-2_506-938-H609WDepositorInsertNcQde-2_506-938-H609W
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NcQDE2 _506-938-K634A_M
Plasmid#146966PurposeBacterial Expression of NcQde-2_506-938-K634ADepositorInsertNcQde-2_506-938-K634A
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NcQDE2 _506-938-R895A_M
Plasmid#146967PurposeBacterial Expression of NcQde-2_506-938-R895ADepositorInsertNcQde-2_506-938-R895A
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NcQDE2 _506-938-Y595L_M
Plasmid#146968PurposeBacterial Expression of NcQde-2_506-938-Y595LDepositorInsertNcQde-2_506-938-Y595L
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only