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Plasmid#79709PurposeThis plasmid encodes the kinase domain of BMPR2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertBMPR2 (BMPR2 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-3xNLS-OptoJNKi-dsm
Plasmid#89743PurposeExpression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor JIP11DepositorInsertNLS-OptoJNKi dark-state mutant
UseFluorescent proteinTagsmCherryExpressionMammalianMutationC450A mutation of AsLov2JalphaPromoterCMVAvailable sinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-3xNLS-OptoJNKi-lsm
Plasmid#89742PurposeExpression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor JIP11DepositorInsertNLS-OptoJNKi lit-state mutant
UseFluorescent proteinTagsmCherryExpressionMammalianMutationI539E mutation of AsLov2JalphaPromoterCMVAvailable sinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ223-STU
Plasmid#138109PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized, catalytically deactivated AsCas12a-SRDX repressor without promoter and terminatorDepositorInsertdAsCas12a-SRDX
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantMutationPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
EPHB3_HUMAN_D0
Plasmid#79732PurposeThis plasmid encodes the kinase domain of EPHB3. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertEPHB3 (EPHB3 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
EPHA3_HUMAN_D0
Plasmid#79708PurposeThis plasmid encodes the kinase domain of EPHA3. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertEPHA3 (EPHA3 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
EPHB2_HUMAN_D0
Plasmid#79697PurposeThis plasmid encodes the kinase domain of EPHB2. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertEPHB2 (EPHB2 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
KC1E_HUMAN_D0
Plasmid#79724PurposeThis plasmid encodes the kinase domain of KC1E. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertKC1E (CSNK1E Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
DAPK3_HUMAN_D0
Plasmid#79721PurposeThis plasmid encodes the kinase domain of DAPK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertDAPK3 (DAPK3 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT MEK5-DD-mRFP1
Plasmid#47547PurposeGateway entry vector for an inducible mRFP1-tagged constitutively active MEK5 mutantDepositorInsertMEK5-DD (MAP2K5 Human)
UseEntry vectorTagsmRFPExpressionMutationS311D, T315DPromoterAvailable sinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
Rabbit Cav 2.2 W391A pMT2
Plasmid#58734Purposeexpression of rabbit CaV2.2 calcium channels with a mutation (W391) in the α1-interacting domain (AID) in the I-II loop of CaV2.2DepositorInsertCav2.2 (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationW391APromoterSV40Available sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYPQ239-RVR
Plasmid#108863PurposeFnCpf1 Gateway entry plasmid with N623R, K629R and N633R mutationsDepositorInsertFnCpf1-RVR (FnCpf1 with N623R, K629R and N633R mutations)
UseCRISPR; Gateway compatible cpf1 entry cloneTagsNLSExpressionPlantMutationN623R, K629R and N633R, Cpf1 is rice codon optimiā¦PromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
MK11_HUMAN_D0
Plasmid#79692PurposeThis plasmid encodes the kinase domain of MK11. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertMK11 (MAPK11 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
FES_HUMAN_D0
Plasmid#79686PurposeThis plasmid encodes the kinase domain of FES. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertFES (FES Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAPK3_HUMAN_D0
Plasmid#79702PurposeThis plasmid encodes the kinase domain of MAPK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertMAPK3 (MAPK3 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xHA-MKK6-EE
Plasmid#47544PurposeGateway entry vector for an inducible 3xHA-tagged constitutively active MKK6 mutantDepositorInsert3xHA-MKK6-EE (MAP2K6 Human)
UseEntry vectorTags3XHAExpressionMutationS207E, T211EPromoterAvailable sinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pENTR-FLAG-HAND1-T2A-mTagBFP2
Plasmid#223192PurposeEntry vector containing FLAG-HAND1 with a T2A-mTagBFP2 reporter (attL flanked)DepositorInsertHAND1 (HAND1 Human)
UsePromoterless entry vector for gateway cloningTagsFLAG and T2A-mTagBFP2ExpressionBacterialMutationPromoterNoneAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A 2xHA GPR153
Plasmid#226808PurposeGateway entry vector for 2xHA GPR153DepositorInsertGPR153 (GPR153 Human)
UseGateway entryTags2xHA tag followed by a GDPPVAT linkerExpressionMutationSequence optimized from NP_997253.2PromoterAvailable sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A 2xHA LTB4R
Plasmid#226806PurposeGateway entry vector for 2xHA LTB4RDepositorInsertLTB4R (LTB4R Human)
UseGateway entryTags2xHA tag followed by a GDPPVAT linkerExpressionMutationSequence optimized from NP_858043.1PromoterAvailable sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKM 1995
Plasmid#217826PurposeExpresses 1554 bp fragment of HY4 gene in A. thaliana. This HY4 fragment shows high homology to the phr genes in prokaryotes.DepositorInsertHY4 (CRY1 Mustard Weed)
UseTagsMaltose-binding proteinExpressionBacterialMutationPromoterAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only