-
Plasmid#208627Purposefor E. coli expression of truncated human RPL3 (ENST00000216146), aa299~398, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 299~398 (RPL3 Human)
UseTags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 299~398, other residues not includedPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-212~285-mEGFP(A206K)-TwStrep
Plasmid#208629Purposefor E. coli expression of truncated human RPL3 (ENST00000216146), aa212~285, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 212~285 (RPL3 Human)
UseTags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 212~285, other residues not includedPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NcQDE2 _506-938-del787-838-GGSG_O
Plasmid#147085PurposeBacterial Expression of NcQde-2_506-938-del787-838DepositorInsertNcQde-2_506-938-del787-838
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NcQDE2 _506-938-del787-838-GSG_O
Plasmid#147086PurposeBacterial Expression of NcQde-2_506-938-del787-838DepositorInsertNcQde-2_506-938-del787-838
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pet21a-aGFPnb-minimizer-cys-linker-YbbR-(PAS)5-H6
Plasmid#192789PurposeaGFP nanobody minimizer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labelingDepositorInsertantiGFP nanobody minimizer
UseTagsH6 and ybbRExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Mortierella verticillate (delta prenylation motif)
Plasmid#170788PurposeExpresses pET-15b(+) Mortierella verticillate (delta prenylation motif)DepositorInsertMortierella verticillate PRL
UseTagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lacIAvailable sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Toxoplasma gondii (delta prenylation motif)
Plasmid#170789PurposeExpresses pET-15b(+) Toxoplasma gondii (delta prenylation motif)DepositorInsertToxoplasma gondii PRL
UseTagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lacIAvailable sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Plasmodium falciparum PRL (Δprenylation motif)
Plasmid#166422PurposeExpress pET-15b(+) Plasmodium falciparum PRL (Δprenylation motif)DepositorInsertPlasmodium falciparum PRL
UseTagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lac1Available sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Pythium oligandrum PRL (Δprenylation motif)
Plasmid#166423PurposeExpress pET-15b(+) Pythium oligandrum PRL (Δprenylation motif)DepositorInsertPythium oligandrum PRL
UseTagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lac1Available sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Reticulomyxa filosa PRL (Δprenylation motif)
Plasmid#166424PurposeExpress pET-15b(+) Reticulomyxa filosa PRL (Δprenylation motif)DepositorInsertReticulomyxa filosa PRL
UseTagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lac1Available sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Sphaeforma arctica PRL (Δprenylation motif)
Plasmid#166425PurposeExpress pET-15b(+) Sphaeforma arctica PRL (Δprenylation motif)DepositorInsertSphaeforma arctica PRL
UseTagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lac1Available sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Acytostelium subglobosum PRL (Δprenylation motif)
Plasmid#166415PurposeExpress pET-15b(+) Acytostelium subglobosum PRL (Δprenylation motif)DepositorInsertAcytostelium subglobosum PRL
UseTagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lac1Available sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Caenorhabditis elegans PRL (Δprenylation motif)
Plasmid#166416PurposeExpress pET-15b(+) Caenorhabditis elegans PRL (Δprenylation motif)DepositorInsertcaenorhabditis elegans PRL
UseTagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lac1Available sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Danio rerio PRL (Δprenylation motif)
Plasmid#166417PurposeExpress pET-15b(+) Danio rerio PRL (Δprenylation motif)DepositorInsertDanio rerio PRL (ptp4a3a Zebrafish)
UseTagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lac1Available sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Drosophila melanogaster PRL (Δprenylation motif)
Plasmid#166418PurposeExpress pET-15b(+) Drosophila melanogaster PRL (Δprenylation motif)DepositorInsertDrosophila melanogaster PRL (Prl Fly)
UseTagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lac1Available sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Galdieria sulphuraria PRL (Δprenylation motif)
Plasmid#166419PurposeExpress pET-15b(+) Galdieria sulphuraria PRL (Δprenylation motif)DepositorInsertGaldieria sulphuraria PRL
UseTagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lac1Available sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Monosiga brevicollis PRL (Δprenylation motif)
Plasmid#166420PurposeExpress pET-15b(+) Monosiga brevicollis PRL (Δprenylation motif)DepositorInsertMonosiga brevicollis PRL
UseTagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lac1Available sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Naegleria fowleri PRL (Δprenylation motif)
Plasmid#166421PurposeExpress pET-15b(+) Naegleria fowleri PRL (Δprenylation motif)DepositorInsertNaegleria fowleri PRL
UseTagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lac1Available sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET29b-AviTag-mEGFP-dspB(E184Q W330Y)-6xHis
Plasmid#176575PurposePlasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
UseTagsAvitag, Hexahistidine tag, and mEGFPExpressionBacterialMutationE184Q W330YPromoterT7Available sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET29b-AviTag(K10A)-eGFP-dspB(E184Q)-6xHis
Plasmid#175788PurposePlasmid for overexpression of recombinant AviTagged(nonfunctional), His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
UseTagsAviTag, Hexahistidine tag, and eGFPExpressionBacterialMutationE184QPromoterT7Available sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only