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psicheck2-PHLPP2mut

Plasmid

#203606

Purpose

Reporter plasmid carrying PHLPP2 3'UTR with mutated miR-27b target sequences

Depositor

Hiroyuki Mizuguchi

Article

Sakai et al Commun Biol. 2023 Jun 24;6(1):669. doi:

Insert

PH domain and leucine rich repeat protein phosphatase 2 (PHLPP2 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

miR-27b target sequences in the 3'UTR were m…

Promoter

SV40

Available since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

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psicheck2-B4GALT3

Plasmid

#203601

Purpose

Reporter plasmid carrying B4GALT3 3'UTR

Depositor

Hiroyuki Mizuguchi

Article

Sakai et al Commun Biol. 2023 Jun 24;6(1):669. doi:

Insert

beta-1,4-galactosyltransferase 3 (B4GALT3 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

nt 1800-2330 3'UTR

Promoter

SV40

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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psicheck2-MAIP1

Plasmid

#203603

Purpose

Reporter plasmid carrying MAIP1 3'UTR

Depositor

Hiroyuki Mizuguchi

Article

Sakai et al Commun Biol. 2023 Jun 24;6(1):669. doi:

Insert

matrix AAA peptidase interacting protein 1 (MAIP1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.2 BBS pcDNA3

Plasmid

#206057

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double Bungarotoxin Binding Site motif in domain II

Depositor

Annette Dolphin

Article

Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-BCR-ABL-p210-ΔST-FLAG

Plasmid

#205623

Purpose

Expression of BCR-ABL oncogene in mammalian cells

Depositor

Pavel Krejčí

Article

Gregor et al Cell Mol Life Sci. 2020 Oct;77(19):3885-

Insert

BCR-ABL oncogene, p210 isoform b3a2

Use

Tags

FLAG

Expression

Mammalian

Mutation

deleted ST domain

Promoter

CMV

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-BCR-ABL-p210-ΔIDR3-FLAG

Plasmid

#205624

Purpose

Expression of BCR-ABL oncogene in mammalian cells

Depositor

Pavel Krejčí

Article

Gregor et al Cell Mol Life Sci. 2020 Oct;77(19):3885-

Insert

BCR-ABL oncogene, p210 isoform b3a2

Use

Tags

FLAG

Expression

Mammalian

Mutation

deleted IDR3 domain

Promoter

CMV

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-BCR-ABL-p210-ΔFA-FLAG

Plasmid

#205625

Purpose

Expression of BCR-ABL oncogene in mammalian cells

Depositor

Pavel Krejčí

Article

Gregor et al Cell Mol Life Sci. 2020 Oct;77(19):3885-

Insert

BCR-ABL oncogene, p210 isoform b3a2

Use

Tags

FLAG

Expression

Mammalian

Mutation

deleted FA domain

Promoter

CMV

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-BCR-ABL-p210-ΔDH-FLAG

Plasmid

#205629

Purpose

Expression of BCR-ABL oncogene in mammalian cells

Depositor

Pavel Krejčí

Article

Gregor et al Cell Mol Life Sci. 2020 Oct;77(19):3885-

Insert

BCR-ABL oncogene, p210 isoform b3a2

Use

Tags

FLAG

Expression

Mammalian

Mutation

deleted DH domain

Promoter

CMV

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-BCR-ABL-p210-KD-FLAG

Plasmid

#205626

Purpose

Expression of BCR-ABL oncogene in mammalian cells

Depositor

Pavel Krejčí

Article

Gregor et al Cell Mol Life Sci. 2020 Oct;77(19):3885-

Insert

BCR-ABL oncogene, p210 isoform b3a2

Use

Tags

FLAG

Expression

Mammalian

Mutation

kinase-dead (KD; K271H)

Promoter

CMV

Available since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-BCR-ABL-p210-Y177F-FLAG

Plasmid

#205627

Purpose

Expression of BCR-ABL oncogene in mammalian cells

Depositor

Pavel Krejčí

Article

Gregor et al Cell Mol Life Sci. 2020 Oct;77(19):3885-

Insert

BCR-ABL oncogene, p210 isoform b3a2

Use

Tags

FLAG

Expression

Mammalian

Mutation

Y177F

Promoter

CMV

Available since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-BCR-ABL-p210-ΔAB-FLAG

Plasmid

#205628

Purpose

Expression of BCR-ABL oncogene in mammalian cells

Depositor

Pavel Krejčí

Article

Gregor et al Cell Mol Life Sci. 2020 Oct;77(19):3885-

Insert

BCR-ABL oncogene, p210 isoform b3a2

Use

Tags

FLAG

Expression

Mammalian

Mutation

deleted AB domain

Promoter

CMV

Available since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-BCR-ABL-p210-ΔTK-FLAG

Plasmid

#205630

Purpose

Expression of BCR-ABL oncogene in mammalian cells

Depositor

Pavel Krejčí

Article

Gregor et al Cell Mol Life Sci. 2020 Oct;77(19):3885-

Insert

BCR-ABL oncogene, p210 isoform b3a2

Use

Tags

FLAG

Expression

Mammalian

Mutation

deleted tyrosine kinase (TK) domain

Promoter

CMV

Available since

Sept. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4.15_pT-REX17

Plasmid

#195497

Purpose

Firefly luciferase promoter expression vector driven only by the promoter element of human lncRNA T-REX17

Depositor

Alexander Meissner

Article

Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

Insert

Promoter region of lncRNA T-REX17

Use

Tags

luc2-hPEST

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pShuttle.CC9

Plasmid

#192931

Purpose

Gateway compatible vector with BsaI cloning site and Cre-2A-Cas9 expression

Depositor

Thorsten Stiewe

Article

Merle et al Mol Cancer. 2022 Oct 3;21(1):191. doi: 1

Type

Empty backbone

Use

Gateway-compatible cloning vector

Tags

Expression

Mutation

Promoter

CMV

Available since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

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ABHD2-3'UTR-WT

Plasmid

#190094

Purpose

examine the activity of ABHD2 3'UTR

Depositor

Bowen Liu

Article

Liu et al Commun Biol. 2022 Aug 13;5(1):816. doi:

Insert

ABHD2 3' UTR Wild Type (ABHD2 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pNHT7::UMSBP::sNluc2_ORF2

Plasmid

#186766

Purpose

An mRNA production vector encoding planarian codon-optimized sNluc2 with UMSBP 5' and 3' UTRs beginning at a start codon further downstream of the endogenous gene's start codon..

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2308-Tier1-pGL3-PPGK-NLuc_TB

Plasmid

#169551

Purpose

Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

pGL3-insulator-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette

Use

Tags

Expression

Mammalian

Mutation

Promoter

PPGK

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pKLD-CAG-DYSF-CPL

Plasmid

#131458

Purpose

Encodes Dysferlin protein (Therapeutic gene) protein to cure LGMD2B along with luciferase enzyme for in vivo live imaging

Depositor

Michele Calos

Article

Guha et al Mol Ther Methods Clin Dev. 2019 Oct 14;1

Insert

DYSF (DYSF Human)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

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p406 – TDH3-ER-FlucDM-GFP11-Ura

Plasmid

#177731

Purpose

DM Firefly luciferase reporter fused to a KAR2 signal sequence for ER targeting and HDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus in yeast plasmid with

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

FlucDM

Use

Tags

GFP11, HA, HDEL, and KAR2 Signal Sequence

Expression

Bacterial and Yeast

Mutation

R188Q, R261Q (Destabilized Mutant)

Promoter

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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dre-cdk2 3'UTR sicheck2

Plasmid

#121123

Purpose

luciferase assay

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

dre-cdk2 3'UTR (cdk2 Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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