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Plasmid#195194Purposedual gRNAs for deletion of OR2M4 in a third generation Cas9 backbone with GFPDepositorInsertOR2M4 dual gRNA (OR2M4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
OR6K6_Deletion_gRNA3
Plasmid#195197Purposedual gRNAs for deletion of OR6K6 in a third generation Cas9 backbone with GFPDepositorInsertOR6K6 dual gRNA (OR6K6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTMB400_ZFcharm_Prnp_DPM
Plasmid#220847PurposeAAV genome expressing ZFcharm targeting Prnp; double perfect match self-silencing binding siteDepositorInsertZFcharm
UseAAVTagsHAtagExpressionMammalianMutationN/APromoterEFSAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTMB399_ZFcharm_Prnp_SPM
Plasmid#220846PurposeAAV genome expressing ZFcharm targeting Prnp; single perfect match self-silencing binding siteDepositorInsertZFcharm
UseAAVTagsHAtagExpressionMammalianMutationN/APromoterEFSAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTMB340_ZFcharm_Kv1_Prnp_DPM
Plasmid#220843PurposeAAV genome expressing ZFcharm Kv1 targeting Prnp; double perfect match self-silencing binding siteDepositorInsertZFcharm Kv1
UseAAVTagsHAtagExpressionMammalianMutationN/APromoterEFSAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
MSCV-human Igf2bp2-IRES-GFP
Plasmid#91890PurposeRetroviral expression of human Igfbp2DepositorInsertIgf2bp2 (IGF2BP2 Human)
UseRetroviralTagsIRES-EGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
MARK3_HUMAN_D0
Plasmid#79740PurposeThis plasmid encodes the kinase domain of MARK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertMARK3 (MARK3 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-GFP-IMP2-2
Plasmid#42175DepositorInsertInsulin-like growth factor 2 mRNA binding protein 2-2 (IGF2BP2 Human)
UseTagsGFPExpressionMammalianMutationPromoterT7Available sinceJune 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
Beta-2-adrenergic receptor-CFP
Plasmid#55794PurposeBeta-2-adrenergic receptor tagged with ECFP on carboxyl terminusDepositorInsertBeta-2-adrenergic receptor-ECFP (ADRB2 Aequorea victoria, Human)
UseTagsECFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pROF346
Plasmid#155331PurposeBinary vector for expression of PULSE system (under the control of AtUbi10 promoter) controlling Venus-H2B. It constitutively expresses Cerulean-NLS and a plant selection cassette.DepositorInsertRB_KanR_Tnos-NLS-PIF6-E-PUbi10_Tnos-nCerulean-PUbi10_Tnos-NLS-VP16-PhyB-PUbi10_Tnos-EL222-NLS-SRDX-PUbi10_T35S-nVenus-POpto_LB
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
BM00369
Plasmid#155338PurposeBinary vector for expression of PULSE system (under the control of CaMV35S promoter) controlling GUS. It constitutively expresses dsRed and a plant selection cassette.DepositorInsertRB_KanR_Tnos-NLS-PIF6-E-P35S_Tnos-dsRed-PUbi10_Tnos-NLS-VP16-PhyB-P35S_Tnos-EL222-NLS-SRDX-P35S_T35S-GUS-POpto_LB
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL4.0-TERT G228A
Plasmid#84926Purposeluciferase reporter for mutant TERT promoterDepositorInsertTERT promoter (TERT Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationG228APromoterTERTAvailable sinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLX317-VRK1-K179E
Plasmid#199644PurposeExpresses kinase-inactive VRK1 (K179E)DepositorInsertVRK1 (VRK1 Human)
UseLentiviralTagsExpressionMutationK179EPromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-7:SEC61B-mEGFP
Plasmid#87426PurposeHomology arms and linker-mEGFP sequence for N-terminus tagging of human SEC61BDepositorInsertSEC61B Homology Arms with mEGFP-linker (SEC61B Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceMarch 15, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
DYR1A_HUMAN_D0
Plasmid#79690PurposeThis plasmid encodes the kinase domain of DYR1A. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertDYRK1A (DYRK1A Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
ABL1_HUMAN_D0
Plasmid#79727PurposeThis plasmid encodes the kinase domain of ABL1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertABL1 (ABL1 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-SwitchON-sgVRK1
Plasmid#199638PurposeTamoxifen-inducible expression of sgRNA targeting VRK1DepositorInsertN/A (VRK1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
LentiRCas9-CUG
Plasmid#104183PurposeLentiviral transfer vector that carries U6-driven sgRNA targeting CUG repeats using a modified scaffold (Chen et al. Cell 2013) and CMV-driven PIN-dCas9. Derived from LentiCRISPR v2 (Zhang lab)DepositorInsertU6-CUGsgRNA, EFS-PIN-dCas9
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-mKOR
Plasmid#179285PurposeExpress mouse kappa opioid receptor (GFP tagged) in mammalian cellsDepositorInsertkappa opioid receptor (Oprk1 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHis-LMW_FGF2
Plasmid#157657PurposeExpresses FGF2 in E.coli cellDepositorInsertFibroblast growth factor 2 (FGF2 Human)
UseTagsHis tagExpressionBacterialMutationchanged Cystein 211,229 to serine, deleted amino …PromoterAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only