-
Plasmid#145717PurposeBacterial Expression plasmid for SARS-CoV-2 nsp3DepositorInsertSARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBad33_ISAba30_reRNA
Plasmid#205323PurposeBacterial expression of ISAba30 TnpB and its reRNADepositorInsertISAba30 TnpB
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBad33_ISDge10_reRNA
Plasmid#205340PurposeBacterial expression of ISDge10 TnpB and its reRNADepositorInsertISDge10 TnpB
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLys-M1
Plasmid#109382PurposeArabinose inducible VioB-mCherry expression (stronger RBS). Used to test burden response in E. coliDepositorInsertpARABAD-VioB-mCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJC225
Plasmid#176883PurposeMoClo Level1 plasmid, allows transition into Cermak, et al., 2017 GG cloning systemDepositorTypeEmpty backboneUseCloningTagsExpressionMutationPromoterAvailable sinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCA24N_6xHis-TEV-rpsA
Plasmid#83585PurposeExpress E. coli ribosomal protein S1 with a TEV-cleavable, N-terminal His tagDepositorInsertrpsA (rpsA Escherichia coli)
UseTagsHis tagExpressionBacterialMutationPromoterp-T5 LacAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRU293
Plasmid#167688PurposeFinal destination vector for single Gateway LR reactionDepositorInsertpEC1.2::Cas9i
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046955
Plasmid#145616PurposeBacterial Expression plasmid for RNA-dependent RNA polymeraseDepositorInsertRNA-dependent RNA polymerase (Nsp12) (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;FLAG_2ExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDGE1108
Plasmid#202772PurposeCRISPR-KO in Arabidopsis thalianaDepositorInsertsFAST marker
pRPS5a:zCas9_ttriple
ccdB cassette (BsaI-excisable)
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMIN-VS1
Plasmid#197783PurposeLevel 2 destination vector: pMIN-VS1 backboneDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRMCE{G418R-5FCS-GMRWhite}-Vasa-FC31
Plasmid#165887PurposeG418 resistant FC31 integrase mediated RMCE cassette for upgrading MiMic insertionsDepositorInsertAll-in-one FC31 RMCE Cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA-GFP
Plasmid#133009PurposepFA carrying a sfGFP geneDepositorInsertsfGFP
UseTags6xhisExpressionBacterialMutationPromotertetRAvailable sinceMay 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQCXP CMV/TO DEST (w360-1)
Plasmid#17386PurposeRetroviral Gateway destination vector, Expression, CMV/TO promoter, PuroDepositorTypeEmpty backboneUseRetroviral; Destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
TAF3 PHD_pGEX-6P-2
Plasmid#92100PurposeExpresses GST fusion TAF3 PHD domain proteinDepositorInsertTAF3 PHD domain (TAF3 Human)
UseTagsGST TagExpressionBacterialMutationPromotertac promoterAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJB38-NWMN2930
Plasmid#84457PurposepJB38 plasmid containing the S.aureus Newman genetic region between genes 29 and 30DepositorInsertStaphylococcus aureus NWMN genomic region NWMN29-30
UseTagsExpressionMutationPromoterAvailable sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA2
Plasmid#145370PurposeGateway destination vector for cell-free and mamalian expression with N-term 8xHis-tag and mCherry tagDepositorTypeEmpty backboneUseTagsHis and mCherryExpressionBacterialMutationPromoterAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB301
Plasmid#68629PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pWS481
Plasmid#123029PurposeLexA UASDepositorInsertLexO (6x) [2a]
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPTK002-2-pGAP
Plasmid#84961PurposeType 2, pGAP, Glyceraldehyde-3-phosphate dehydrogenase promoterDepositorInsertGlyceraldehyde-3-phosphate dehydrogenase promoter
UseSynthetic BiologyTagsExpressionYeastMutationBsaI site removed (1805 c to g)PromoterNonAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHnCBS1D
Plasmid#52065Purposeexpresses nine genes from the H. neapolitanus carboxysome operon and CSOS1D; IPTG-inducibleDepositorInsertCbbL, CbbS, CSOS2, CSOS3, CSOS4A, CSOS4B, CSOS1C, CSOS1A, CSOS1B, CSOS1D
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 4, 2014AvailabilityAcademic Institutions and Nonprofits only