Addgene

Showing: 3721 - 3740 of 22178 results

Results per page:

Sort results by:

pLBM0040

Plasmid

#172819

Purpose

Level 2 module containing HSP21-nYFP deltaPTAC5-cYFP

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

[35S-P19][35S-CTPSSU-mHSP21-3XFLAGnYFP][35S-CTPSSU-deltamPTAC5-cYFPX3HA][35S-SP_SV40-CFP]

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLBM0030

Plasmid

#172818

Purpose

Level 2 module containing HSP21-nYFP PTAC5-cYFP

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

[35S-P19][35S-CTPSSU-mHSP21-3XFLAGnYFP][35S-CTPSSU-mPTAC5-cYFPX3HA][35S-SP_SV40-CFP]

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLBM0020

Plasmid

#172817

Purpose

Level 2 module containing HSP21-nYFP HSP21-cYFP

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

[35S-P19][35S-CTPSSU-mHSP21-3XFLAGnYFP][35S-CTPSSU-mHSP21-cYFPX3HA][35S-SP_SV40-CFP]

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLBM0060

Plasmid

#172821

Purpose

Level 2 module containing HSP21-nYFP HSP21-cYFP OEP7-mTRQ

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

[35S-P19][35S-CTPSSU-mHSP21-3XFLAGnYFP][35S-CTPSSU-mHSP21-cYFPX3HA][OEP7_mTRQ]

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLBM0070

Plasmid

#172822

Purpose

Level 2 module containing mCHERRY-nYFP HSP21-cYFP OEP7-mTRQ

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

[35S-P19][35S-CTPSSU-mCHERRY-3XFLAGnYFP][35S-CTPSSU-mHSP21-cYFPX3HA][OEP7_mTRQ]

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
RNAPII_CTD27-52_N2A

Plasmid

#98677

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with N2A

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

N1775A, N1782A

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
RNAPII_CTD27-52_del1961-1970

Plasmid

#98680

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with del1961-1970

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

del1961-1970

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
RNAPII_CTD27-52_N2A_S1966C

Plasmid

#98682

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with N2A and S1966C

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

N1775A, N1782A, S1966C

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW8ENdeI-cHAIAA7-spo5DSR

Plasmid

#49873

Purpose

for Cre-lox cassette exchange of C-terminal HA-auxin-inducible degron tagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

IAA17 degron tag

Expression

Mutation

Promoter

Available since

Jan. 21, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1-HSD17B13-WT-FLAGC

Plasmid

#184501

Purpose

Expresses human HSD17B13 with a C-terminal FLAG-GFP tag. Confers G418 resistance.

Depositor

Sébastien Britton

Article

Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

Insert

17-beta-hydroxysteroid dehydrogenase 13 (HSD17B13 Human)

Use

Tags

FLAGC-GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1-HPGD-FLAGC

Plasmid

#161915

Purpose

Expresses human HPGD with a C-terminal FLAG-GFP tag. Confers G418 resistance.

Depositor

Sébastien Britton

Article

Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

Insert

HPGD (HPGD Human)

Use

Tags

FLAG-GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
FKBP-AP180-GFP

Plasmid

#186577

Purpose

Expression of the fusion protein FKBP-AP180-GFP. Uses rat AP180 residues 328-896.

Depositor

Stephen Royle

Article

Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

Insert

AP180 (Snap91 Rat)

Use

Tags

FKBP N-terminal GFP C-terminal

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-link-GFPγ-SpHis5

Plasmid

#60781

Purpose

Yeast C-terminal tagging vector, which creates gene fusions with GFPγ using SpHis5 selection

Depositor

Linda Huang

Article

Slubowski et al Yeast. 2015 Jan 22. doi: 10.1002/yea.306

Insert

GFPγ

Use

Yeast integrating

Tags

Expression

Mutation

T65C S72A V163A I167T L231H

Promoter

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
EC71171

Plasmid

#154069

Purpose

Level 0 Golden Gate vector, CRE with U5 intron at 254bp, in pICH41308 backbone

Depositor

Cristobal Uauy

Article

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Insert

CRE_U5intron

Use

Synthetic Biology

Tags

Expression

Mutation

All BsaI, Esp3I and BPiI restriction sites were r…

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-AMPKsub-YPet-NES

Plasmid

#84635

Purpose

Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

AMPKsub-YPet-NES

Use

Tags

6xHis, Nuclear export signal (NES), T7 tag (gene …

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
EC71139

Plasmid

#154062

Purpose

Level 0 Golden Gate vector, containing the ZmUbi promoter and 5' UTR with Level 0.5-compatible overhangs (Barley Construct)

Depositor

Cristobal Uauy

Article

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Insert

pZmUbi

Use

Synthetic Biology

Tags

Expression

Mutation

All BsaI, Esp3I and BpiI restriction sites were r…

Promoter

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-Hyg-FHIT

Plasmid

#16410

Depositor

Bert Vogelstein

Article

Kohli et al Nucleic Acids Res. 2004 . 32(1):e3.

Insert

FHIT Hyg targeting (FHIT Human)

Use

AAV; Adeno-associated viral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2008

Availability

Academic Institutions and Nonprofits only
pET28 His6-mEGFP-D4H

Plasmid

#226373

Purpose

Production of recombinant mEGFP-D4H, a fluorescent probe binding cholesterol

Depositor

Catherine Tomasetto

Article

Eichler et al Methods Mol Biol. 2025;2888:101-118. doi

Insert

D4H (pfoA Clostridium perfringens)

Use

Tags

His6 and monomeric EGFP

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Enterovirus A71 (EV-A71) isolate 4643-TW98 3C protease with non-cleavable C-terminal His AviTag

Plasmid

#228635

Purpose

Bacterial expression of the A71 3C protease for assays that require immobilization using biotin-streptavidin interaction

Depositor

Frank von Delft, ASAP Discovery

Insert

Enterovirus A71 3C protease isoform A

Use

Tags

His-Avi

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Enterovirus A71 (EV-A71) isolate 4643-TW98 2A protease with non-cleavable C-terminal His AviTag

Plasmid

#228634

Purpose

Bacterial expression of the A71 2A protease for assays that require immobilization using biotin-streptavidin interaction

Depositor

Frank von Delft, ASAP Discovery

Insert

Enterovirus Coxsackievirus A71 2A protease

Use

Tags

His-Avi

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge