Addgene

We narrowed to 28,566 results for: uros

Showing: 9301 - 9350 of 28566 results

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pJH75_pCMV_hyCal9

Plasmid

#160420

Purpose

CMV promoter driving HyCal component (HyNaC9)

Depositor

Daniel Colón-Ramos

Article

Hawk et al Nat Commun. 2021 Aug 9;12(1):4795. doi:

Insert

pEZY3 CMV-hyCal9

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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CENP-B mCherry PP1 gamma

Plasmid

#45221

Depositor

Iain Cheeseman, Michael Lampson

Article

Liu et al J Cell Biol. 2010 Mar 22;188(6):809-20.

Insert

CENP-B mCherry PP1 gamma

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7

Available since

May 31, 2013

Availability

Academic Institutions and Nonprofits only

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pGBW-m4134904

Plasmid

#151930

Purpose

Mammalian expression plasmid for SARS-CoV-2 spike prefusion-stabilized

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 spike prefusion-stabilized (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

d1-15 d1209-1273 R682A R685G K986P V987P

Promoter

CMV

Available since

May 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCLX-UBI-mCherry

Plasmid

#114275

Purpose

Ubiquitous expression of the mCherry living color

Depositor

Patrick Salmon

Article

Boitard et al Cell Rep. 2015 Mar 3;10(8):1349-61. doi:

Insert

mCherry

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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LacI INCENP

Plasmid

#45236

Purpose

Target INCENP to lac operator sequence

Depositor

Michael Lampson

Article

Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

Insert

LacI, INCENP-Δcen, mCherry (lacI )

Use

Tags

LacI (lac repressor protein) and mCherry

Expression

Mammalian

Mutation

CEN box deleted from INCENP (aa1-46)

Promoter

t7

Available since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only

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XW54

Plasmid

#65839

Purpose

Punc-4c-NLS-QF-BD-DM-Nzipper-SL2-mCherry-3'UTR-unc-54 for Split Q intersectional method

Depositor

Kang Shen

Article

Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

Insert

NLS-QF-BD-DM-Zipper

Use

Tags

mCherry

Expression

Worm

Mutation

Promoter

Punc-4c

Available since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti-CaMKIIa-hChR2(C128A)-EYFP-WPRE

Plasmid

#20293

Purpose

Lentiviral expression of ChR2 (C128A) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitation

Depositor

Karl Deisseroth

Article

Berndt et al Nat Neurosci. 2009 Feb . 12(2):229-34.

Insert

channelrhodopsin-2

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

changed Cysteine 128 to Alanine

Promoter

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pGEM-T Easy-Gbait-hs-Cre

Plasmid

#122562

Purpose

Integration of Cre sequence using the CRISPR/Cas9 system.

Depositor

Marnie Halpern

Article

Choi et al Elife. 2021 Dec 8;10:e72345. doi: 10.755

Insert

Gbait-hsp70-Cre-SV40pA

Use

Cloning

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCLX-UBI-Tred

Plasmid

#27246

Depositor

Patrick Salmon

Article

Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

Insert

TurboRFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-Myc-rSyntenin1

Plasmid

#89435

Purpose

mammalian expression of rat Syntenin1

Depositor

Fritz Rathjen

Article

Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

Insert

SDCBP

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST-APIC-LexAGAD

Plasmid

#112807

Purpose

gateway cloning destination vector to express enhancer driven LexAGAD in Drosophila

Depositor

Chun Han

Article

Poe et al Proc Natl Acad Sci U S A. 2017 Sep 19;11

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pShGSK3bE

Plasmid

#37526

Depositor

Jerry Crabtree

Article

Graef et al Nature. 1999 Oct 14. 401(6754):703-8.

Insert

GSK3b (GSK3B Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

SRa promoter

Available since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only

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pTorPE-Y-GECO2f

Plasmid

#105065

Purpose

A yellow fluorescent Ca2+ indicator variant with low binding affinity and fast kinetics for expression in bacterial

Depositor

Robert Campbell

Article

Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

Insert

Y-GECO2f

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

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pUAS-MmTFP13xmyc

Plasmid

#86371

Purpose

membrane targeted Tfp1 expression in zebrafish

Depositor

Rachel Wong

Article

Yoshimatsu et al Nat Commun. 2016 Feb 3;7:10590. doi: 10.

Insert

mTfp1

Use

Zebrafish

Tags

3xMyc and GAP43 membrane targeting

Expression

Mutation

Promoter

UAS

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2 hSmad1-GM

Plasmid

#22992

Depositor

Edward De Robertis

Article

Fuentealba et al Cell. 2007 Nov 30. 131(5):980-93.

Insert

Smad1 (SMAD1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S210A ,T202A, S191A, S198A

Promoter

Available since

June 17, 2010

Availability

Academic Institutions and Nonprofits only
pLpC-L-IFP-F[1] (mammalian)

Plasmid

#52901

Purpose

Binary retroviral pl. (puromycin). MCS:(GGGGS)X 2 + IFP-F[1] (ClaI/EcoRI). Insert can be subcloned between the MCS. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

L-IFP-F[1]

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Unknown

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only
pLpC-L-IFP-F[2] (mammalian)

Plasmid

#52902

Purpose

Binary retroviral pl. (puromycin). MCS:(GGGGS)X 2 + IFP-F[2] (ClaI/EcoRI). Insert can be subcloned between the MCS. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

L-IFP-F[2]

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Unknown

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only
pRSET FLIPE-10u

Plasmid

#13538

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E A207R

Use

Tags

ECFP and Venus

Expression

Bacterial

Mutation

A207R in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pMSCV Cyclin D2 long-wt

Plasmid

#21646

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

Cyclin D2 (CCND2 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pMSCV Cyclin D2-short

Plasmid

#21655

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

Cyclin D2 (CCND2 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH181.3

Plasmid

#135076

Purpose

Coryne. glutamicum HA production plasmid

Depositor

Josef Altenbuchner

Article

Hoffmann et al J Appl Microbiol. 2014 Sep;117(3):663-78

Insert

hasA-hasC

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pBN67

Plasmid

#86714

Purpose

DamID control plasmid for C. elegans

Depositor

Peter Askjaer

Article

Gonzalez-Aguilera et al Genome Biol. 2014 Feb 3;15(2):R21. doi:

Insert

Phsp16.41::GFP::MYCDam

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pUHD-AID

Plasmid

#121066

Purpose

vector expresses AID-fusion protein under TRE promoter and with puromycin resistance selection

Depositor

Randy Poon

Article

Ng et al Cell Cycle. 2018 Dec 23. doi: 10.1080/15

Type

Empty backbone

Use

Tags

AID

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pISH-Tshz2

Plasmid

#74361

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Tshz2. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

teashirt zinc finger family member 2 (Tshz2 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-CaMKIIa-hChR2(C128T)-EYFP-WPRE

Plasmid

#20295

Purpose

Lentiviral expression of ChR2 (C128T) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitation

Depositor

Karl Deisseroth

Article

Berndt et al Nat Neurosci. 2009 Feb . 12(2):229-34.

Insert

channelrhodopsin-2

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

changed Cysteine 128 to Threonine

Promoter

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
FUp95bGW (B6)

Plasmid

#74015

Purpose

lentiviral expression of shRNA resistant Psd95-beta-EGFP fusion

Depositor

Robert Malenka, Weifeng Xu, Oliver Schluter

Article

Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

Insert

Psd95 beta isoform

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

shRNA resistant Psd95

Promoter

hUbC

Available since

March 31, 2016

Availability

Academic Institutions and Nonprofits only
mCherryC1-PIPKI{gamma}661

Plasmid

#29584

Depositor

Anna Huttenlocher

Article

Lokuta et al Mol Biol Cell. 2007 Dec . 18(12):5069-80

Insert

phosphatidylinositol phosphate kinase type I gamma

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

May 23, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSET FLIPE-1m

Plasmid

#13540

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E A207W

Use

Tags

ECFP and Venus

Expression

Bacterial

Mutation

A207W in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pBP-mTAZ-NFLAG

Plasmid

#31791

Depositor

Michael Yaffe

Article

Hong et al Science. 2005 Aug 12;309(5737):1074-8.

Insert

TAZ (Wwtr1 )

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only
pISH-Cck

Plasmid

#105989

Purpose

To prepare riboprobe for in situ hybridization

Depositor

Peter Mombaerts

Article

Walz et al J Neurobiol. 2006 Jul . 66(8):835-46.

Insert

cholecystokinin (Cck Mouse)

Use

To generate riboprobe

Tags

Expression

Mutation

Promoter

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Mis12 INCENP Δcen mCherry

Plasmid

#45228

Depositor

Susanne Lens, Michael Lampson

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

Mis12 INCENP Δcen mCherry

Use

Tags

VSVG

Expression

Mammalian

Mutation

CEN box deleted from INCENP (aa1-46)

Promoter

Available since

July 16, 2013

Availability

Academic Institutions and Nonprofits only
pSuper mouse Dicer2

Plasmid

#14777

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 Apr 1. ():.

Insert

Dicer shRNA (Dicer1 Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
FH95pUGW (B3)

Plasmid

#74012

Purpose

lentiviral expression of EGFP and Psd95 shRNA

Depositor

Robert Malenka, Weifeng Xu, Oliver Schluter

Article

Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

Insert

Psd95

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1 shMTH1-1

Plasmid

#21297

Depositor

Bob Weinberg

Article

Rai et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

shMTH1-1 (NUDT1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 29, 2009

Availability

Academic Institutions and Nonprofits only
pSuper mouse Drosha2

Plasmid

#14775

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 Apr 1. ():.

Insert

Drosha shRNA (Drosha Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
GST-SEPT5 WT

Plasmid

#27269

Depositor

Harish Pant

Article

Amin et al J Neurosci. 2008 Apr 2. 28(14):3631-43.

Insert

Septin 5 (SEPTIN5 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pSuper mouse Drosha3

Plasmid

#14776

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 Apr 1. ():.

Insert

Drosha shRNA (Drosha Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3-TRPgamma

Plasmid

#83271

Purpose

Mammalian expression plasmid for Drosophila TRPgamma cDNA

Depositor

Craig Montell

Article

Xu et al Neuron. 2000 Jun;26(3):647-57.

Insert

TRPgamma (Trpgamma Fly)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pISH-Pax6

Plasmid

#74350

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Pax6. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

paired box 6 (Pax6 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

April 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pEAK10-His-Myc-Kal9

Plasmid

#25441

Depositor

Betty Eipper

Article

Johnson et al J Biol Chem. 2000 Jun 23. 275(25):19324-

Insert

Kal9

Use

Tags

His and Myc

Expression

Mammalian

Mutation

Promoter

Available since

July 15, 2010

Availability

Academic Institutions and Nonprofits only
pQCXP CMV/TO empty (w368-1)

Plasmid

#17393

Purpose

Retroviral Empty control vector, CMV/TO promoter, Puro

Depositor

Paul Kaufman, Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST-APIC-Flp1

Plasmid

#112806

Purpose

gateway cloning destination vector to express enhancer driven Flip1 in Drosophila

Depositor

Chun Han

Article

Poe et al Proc Natl Acad Sci U S A. 2017 Sep 19;11

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1 shMTH1-2

Plasmid

#21298

Depositor

Bob Weinberg

Article

Rai et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

shMTH1-2 (NUDT1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 29, 2009

Availability

Academic Institutions and Nonprofits only
pBRIT Pax7 216C

Plasmid

#17523

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Insert

Pax7 (Pax7 Mouse)

Use

Cre/Lox and Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Deletion of aa 1-215

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pEAK10-His-Myc-Kal5

Plasmid

#25440

Depositor

Betty Eipper

Article

Johnson et al J Biol Chem. 2000 Jun 23. 275(25):19324-

Insert

Kal5

Use

Tags

His and Myc

Expression

Mammalian

Mutation

Promoter

Available since

July 15, 2010

Availability

Academic Institutions and Nonprofits only
SEPT11 sh1 3m shRNA

Plasmid

#122021

Purpose

Negative control shRNA for SEPT11 sh1 shRNA (plasmid 122020).

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 shRNA (Sept11 Rat)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSox2-bd::FP

Plasmid

#34703

Depositor

Hollis Cline

Article

Bestman et al J Comp Neurol. 2012 Feb 1;520(2):401-33.

Insert

eGFP

Use

Xenopus expression

Tags

165-bp minimal FGF4 promoter, 6x Sox2/Oct3-4 tran…

Expression

Mutation

Promoter

Available since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF204

Plasmid

#121656

Purpose

U6-sgRNA EFS-Cas9-wt-P2A-Puro

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Insert

Cas9

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAD424-rSyntenin1

Plasmid

#89425

Purpose

Two-hybrid system

Depositor

Fritz Rathjen

Article

Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

Insert

SDCBP

Use

Tags

Gal4 AD

Expression

Yeast

Mutation

Promoter

ADH1

Available since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pQCXP CMV/TO V5-LUC (w476-1)

Plasmid

#17740

Purpose

Retroviral V5-Luciferase expression vector, CMV/TO promoter, Puro

Depositor

Paul Kaufman, Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Firefly Luciferase

Use

Luciferase and Retroviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge

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