-
Plasmid#201955PurposeMammalian expression plasmid of FnCas9ABEmax8.17d base editor with T2A-EGFP and cloning backbone for sgRNADepositorInsertbpNLS-FnCas9ABEmax8.17d-bpNLS-3xHA-T2A-EGFP
UseCRISPRTags3xHA, NLS, and T2A-EGFPExpressionMammalianMutationD11A on FnCas9, V82S, V106W, Q154R on mutant TadAPromoterCbhAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
PX458-3xHA-en15FnCas9
Plasmid#201948PurposeMammalian expression plasmid of enhanced activity FnCas9 variant en15FnCas9 with T2A-EGFP and cloning backbone for sgRNADepositorInsert3xHA-NLS-en15FnCas9-T2A-EGFP
UseCRISPRTags3xHA, NLS, and T2A-EGFPExpressionMammalianMutationE1603H on FnCas9PromoterCbhAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_ATP10B-G671R-N865K
Plasmid#203699Purposeexpresses ATP10B with the pathogenic mutations G671R and N865KDepositorInsertATP10B (ATP10B Human)
UseTagsExpressionMammalianMutationG671R, N865KPromoterAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-U6-sgRNA(OXTR.2)-CMV-eGFP
Plasmid#194016PurposeExpresses a gRNA that targets OXTR coding sequence in multiple (rodent) species and eGFPDepositorInsertssgRNA(Oxtr.2)
eGFP
UseAAV and CRISPRTagsExpressionMammalianMutationPromotercmb and u6Available sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSUMO mouse METAP1 1-79
Plasmid#197397PurposeExpresses SUMO-tagged mouse METAP1, residues 1-79DepositorInsertMETAP1 1-79 (Metap1 Mouse)
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSUMO mouse METAP1 1-59
Plasmid#197396PurposeExpresses SUMO-tagged mouse METAP1, residues 1-59DepositorInsertMETAP1 1-59 (Metap1 Mouse)
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
eIF3a- pMK289 (mAID-mClover-NeoR) plasmid
Plasmid#192235Purposeknockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3aDepositorInserteIF3a-knockin-homology arm-mAID-mClover-NeoR (EIF3A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
eIF3k- pMK289 (mAID-mClover-NeoR) plasmid
Plasmid#192243Purposeknockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3kDepositorInserteIF3k-knockin-homology arm-mAID-mClover-NeoR (EIF3K Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
eIF3k- pMK293 (mAID-mCherry2-Hygro) plasmid
Plasmid#192244Purposeknockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3kDepositorInserteIF3k-knockin-homology arm-mAID-mCherry2-Hygro (EIF3K Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
IL2-AHA740D, E758K-2A-Cherry-RhoAQ63L in pmCherryN1
Plasmid#187288PurposeExpress pmCherry-IL2-AH A740D, E758K-2A-RhoA Q63LDepositorUseTagsmCherryExpressionMammalianMutationAH A740D, E758K, RhoA Q63LPromoterCMVAvailable sinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
S+10subs
Plasmid#167176PurposeExpresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with 10 R mutations in Sf9 cellsDepositorInsertsATP1A1-S+10R
ATP1B1
UseTagsExpressionBacterial and InsectMutationChanged A at 112 to T; E at 116 to D; I at 135 to…PromoterP10 and PHAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
S+12subs
Plasmid#167177PurposeExpresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with 12 R mutations in Sf9 cellsDepositorInsertsATP1A1-S+12R
ATP1B1
UseTagsExpressionBacterial and InsectMutationChanged Q at 111 to R; A at 112 to T; E at 116to …PromoterP10 and PHAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-B.1.351.v4
Plasmid#173788PurposeMammalian expression of SARS-CoV-2 Spike protein South African variant version 4DepositorInsertSpike (S-GSAS-B.1.351 variant version 4) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsHRV3C cleavage site, 8X His tag, 2X Strep-Tag IIExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…PromoterAvailable sinceJan. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
miR3 HsATP10B
Plasmid#171824Purposetransfer plasmid for lentiviral vector production with miR for Hs ATP10BDepositorInsertATP10B (ATP10B Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
miR7 MmATP10B
Plasmid#171825Purposetransfer plasmid for lentiviral vector production with miR for Mm ATP10BDepositorInsertATP10B (Atp10b Mouse)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
miR5 MmATP10B
Plasmid#171791Purposetransfer plasmid for lentiviral vector production with miR for Mm ATP10BDepositorInsertATP10B (Atp10b Mouse)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
miR2 HsATP10B
Plasmid#171790Purposetransfer plasmid for lentiviral vector production with miR for Hs ATP10BDepositorInsertATP10B (ATP10B Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMiR-CIP2A-stop-mut-miR-375-mutA-E
Plasmid#53714PurposeLuciferase reporter assay for CIP2A with mutated firefly stop codon that also has five miR-375 binding site mutationsDepositorInsertCIP2A (CIP2A Human)
UseLuciferaseTagsExpressionMutationMutation on the stop codon of firefly luciferase …PromoterAvailable sinceJuly 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag VRK3
Plasmid#20664DepositorInsertVRK3 (VRK3 Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pJC-TALE-GFP
Plasmid#46148DepositorTypeEmpty backboneUseTagsGFPExpressionInsectMutationPromoterhsp70Available sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pC-0
Plasmid#124421PurposeFull MCS (step 2)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJC-TALE-VP64
Plasmid#46147DepositorTypeEmpty backboneUseTagsVP64 activation domainExpressionInsectMutationPromoterhsp70Available sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJC-TALE-hairy
Plasmid#46145DepositorTypeEmpty backboneUseTagsHairy Repression DomainExpressionInsectMutationPromoterhsp70Available sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pC-0_v2
Plasmid#124422PurposeFull MCS (step 2)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC-0_v5
Plasmid#124425PurposeFull MCS (step 2)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJC-TALE-Kr
Plasmid#46146DepositorTypeEmpty backboneUseTagsKruppel repression domainExpressionInsectMutationPromoterhsp70Available sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTU-A-013
Plasmid#124415PurposeSlot A, empty (step 1)DepositorInsertslot A + terminator + spacer
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC-0_v3
Plasmid#124423PurposeFull MCS (step 2)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
piggyBac-rtTA (4th_Gen)-NGN2-2A-PURO-IRES-SNAP (VK_1018)
Plasmid#209077PurposeNgn2 mediated cortical neuron induction, dox induction is includedDepositorInsertsUseTagsExpressionMammalianMutationPromoterTRE4GAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1A-MYC-IRES-luc
Plasmid#129775PurposeTransposon based vector that expresses human MYC followed by IRES and firefly luciferaseDepositorInserthuman MYC (MYC Human)
UseTagsluciferaseExpressionMammalianMutationPromoterEF1AAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-K-RAS V12
Plasmid#31200DepositorInsertK-Ras (KRAS Human)
UseGateway donor vectorTagsExpressionMutationG12V constitutively activePromoterAvailable sinceJune 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET30-2-PSAT1
Plasmid#83908Purposestable overexpressionDepositorInsertphosphoserine aminotransferase 1 (PSAT1 Human)
UseTags6x His tagsExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag PIK3CG
Plasmid#20574DepositorInsertPIK3CG (PIK3CG Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGL4.22-VEGF-HRE::dLUC
Plasmid#128096PurposeHypoxia response element real-time luciferase reporterDepositorInsertHypoxia response element (x5) from VEGF promoter (VEGFA Human)
UseLuciferaseTagsdrives expression of destabilized luciferase (2CP…ExpressionMammalianMutationPromoterinserted VEGF hypoxia response elements (HRE) (x5)Available sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXJ197-pAAV-PB3'IR-U6-gRNA-directcapture-EF1a-GFP-KASH-PB5'IR
Plasmid#210523PurposeAAV piggybac transposon vector, with GFP-KASH reporter and gRNA cloning site. Compatible with 10X 3' and 5' gRNA capture technology.DepositorInsertsGFP-KASH
U6-gRNA-directcapture
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXJ219-pAAV-PB3'IR-U6-gRNA-EF1a-mtagBFP2-KASH-PB5'IR
Plasmid#210525PurposeAAV piggybac transposon vector, with mtagBFP-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.DepositorInsertsmtagBFP-KASH
U6-gRNA
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag PKN1
Plasmid#20586DepositorInsertPKN1 (PKN1 Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
CLYBL-(Ef1a-SBP-LNGFR-T2A-mApple)-(CAG-rtTA)-(TRE-hNIL))
Plasmid#105842PurposeDonor construct for introduction of hNIL factors and SBP-LNGFR for streptavidin bead enrichmentDepositorInsertsUseCRISPR and TALENTagsExpressionMutationPromoterCAG, EF-1alpha, and TRE3GAvailable sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXJ220-pAAV-PB3'IR-U6-gRNA-EF1a-GFP-KASH-PB5'IR
Plasmid#213481PurposeAAV piggybac transposon vector, with GFP-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.DepositorInsertsGFP-KASH
U6-gRNA
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag GRK5
Plasmid#20495DepositorInsertGRK5 (GRK5 Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag DLG5
Plasmid#20477DepositorInsertDLG5 (DLG5 Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag PKM2
Plasmid#20585DepositorInsertPKM2 (PKM Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag PIK4CB
Plasmid#20578DepositorInsertPIK4CB (PI4KB Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag YES1
Plasmid#20665DepositorInsertYES1 (YES1 Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pXJ218-pAAV-PB3'IR-U6-gRNA-directcapture-EF1a-mtagBFP2-KASH-PB5'IR
Plasmid#213480PurposeAAV piggybac transposon vector, with mtagBFP-KASH reporter and gRNA cloning site. Compatible with 10X 3' and 5' gRNA capture technology.DepositorInsertsmtagBFP-KASH
U6-gRNA-directcapture
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-B.1.1.7
Plasmid#171747PurposeMammalian expression of SARS-CoV-2 Spike protein S-GSAS-B.1.1.7 variant (UK)DepositorInsertSpike (S-GSAS-B.1.1.7 variant) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsHRV3C cleavage site, 8X His tag, 2X Strep-Tag IIExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…PromoterAvailable sinceJuly 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag PKN2
Plasmid#20587DepositorInsertPKN2 (PKN2 Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag HK2
Plasmid#20501DepositorInsertHK2 (HK2 Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag AXL
Plasmid#20428DepositorInsertAXL (AXL Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag PTK2
Plasmid#20610DepositorInsertPTK2 (PTK2 Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only