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Plasmid#132665PurposepyrE-targeted CRISPR-Cas9 control plasmid. Cas9 expression is controlled by the xylR promoter and results in improved conjugation efficiency. Induction with xylose results in a pyrE mutant.DepositorInsertUpstream & Downstream pyrE deletion region
UseE. coli - c. difficile shuttle vectorTagsExpressionMutationPromoterAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
SP-Cas9
Plasmid#62731PurposeExpresses SP-Cas9 protein in bacterial cellsDepositorInsertSP-CAS9
UseCRISPRTagsHis tag and NLSExpressionBacterialMutationCodon optimazed for e.coliPromoterT7Available sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
TurboID-V5_pRS415
Plasmid#107167Purposeexpresses V5-tagged TurboID in the yeast cytosolDepositorInsertTurboID (BirA mutant)
UseTagsExpressionYeastMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…PromoterAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-Chronos-GFP
Plasmid#59170PurposeAAV-mediated expression of Chronos-GFP under the Syn promoterDepositorHas ServiceAAV Retrograde, AAV1, and AAV5InsertChronos-GFP
UseAAVTagsGFPExpressionMammalianMutationPromoterSynAvailable sinceAug. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPBO.ACT001
Plasmid#182711PurposeaTc inducible dCasRxDepositorInsertdCasRx
UseCRISPRTags2x-FLAGExpressionBacterialMutationCatalytically deactivated R295A, H300A, R849A, H8…PromoterpTetAvailable sinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRPF215
Plasmid#106377PurposeC. difficile transposon mutagenesis system. Shuttle vector containing tet-inducible Himar1 transposase and custom ErmR transposonDepositorInsertPtet-himar1-Ter(slpA)-transposon
UseE. coli - c. difficile shuttle vectorTagsExpressionMutationCodon optimised for C. difficilePromoterPtetAvailable sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pM-Cas12a
Plasmid#196291Purpose35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding LbCas12a in place of viral GPDepositorInsertFull length TSWV M antigenome encoding LbCas12a in place of viral GP (cas12a Synthetic)
UseCRISPRTagsFlagExpressionPlantMutationSee depositor comments belowPromoterduplicated cauliflower mosaic virus 35S promoterAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-KLC2-N-18
Plasmid#62747PurposeLocalization: Kinesin Light Chain , N1 Cloning Vector, Excitation:587, Emission:610DepositorInsertKLC2
UseTagsmCherryExpressionMammalianMutationnonePromoterCMVAvailable sinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAVi U6_gRNA CMV_SadCas9-KRAB
Plasmid#214609PurposeAll-in-one AAVi plasmid expressing S. aureus dCas9-KRAB with sgRNA cassetteDepositorInsertsgRNA
SadCas9
UseAAVTagsNP NLS and SV40 NLSExpressionMammalianMutationD10A, N580APromoterCMV and U6Available sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-TRIM28
Plasmid#65397PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorInsertTRIM28 (TRIM28 Human)
UseTagsEmGFP and V5ExpressionMammalianMutationnonePromoterCMVAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
epB_UbC_CymRV5-nls-GFP_DEx2
Plasmid#119906PurposeePiggyBac mammalian expression plasmid for CymR-GFP fusionDepositorInsertCymRV5-nls-GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
UPB-stomach (UPB1)
Plasmid#180784PurposeUnique Projection Barcode (UPB1)-Stomach for Projection-seqDepositorInserthumanized ChR2-H134R (partial)
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
phND2-N106
Plasmid#31780DepositorInsertNeuroD2 (NEUROD2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pThy1-Brainbow3.2
Plasmid#45179DepositorInsertpThy1-Brainbow3.2
UseLinearized for transgenic mouse injectionTagsExpressionMutationPromoterThy1Available sinceNov. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pML107
Plasmid#67639PurposeExpresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformationDepositorInsertsCas9
single guide RNA expression cassette
UseCRISPRTagsExpressionYeastMutationPromoterpSNR52 and pTDH3 (aka GAP promoter)Available sinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBABE-neo-hTERT
Plasmid#1774PurposeRetroviral expression of hTERT. Used to create immortalized cell lines.DepositorInserthTERT (TERT Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2005AvailabilityAcademic Institutions and Nonprofits only -
LentiV2-EF1α-nCas9/RT
Plasmid#210188PurposeExpression of nCas9/RT-P2A-PuroDepositorInsertnCas9/RT-P2A-Puro
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pAXY0001
Plasmid#179834Purposebinary plant vector expressing eYGFPuv and GUS, both driven by a 35S promoterDepositorInserteYGFPuv
UseBinary vector for plant transformationTagsExpressionPlantMutationPromoter35SAvailable sinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDV06_feedback_entry_TagBFP-2A-MCS-2A-mNeonGreen-2A-MCP-ADAR2dd(E488Q)-NES
Plasmid#200402PurposeEntry vector for closed-loop ADAR-based RNA sensors (DART VADAR). Expresses TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon target detectionDepositorInsertClosed-loop DART VADAR sensor entry cassette
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pLV CMV-TERRA
Plasmid#111375PurposeTERRA expression contains approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequencesDepositorInsertTERRA polyA
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Akt-Scramble
Plasmid#175014PurposeNegative-control construct for Akt-STOPS.DepositorInsertmCherry-Akt-Scramble
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUbC-tdTomato
Plasmid#183710PurposetdTomato expression from UbiC promoterDepositorInserttdTomato
UseLentiviralTagsExpressionMutationPromoterUbiCAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDMyc-TNPO1
Plasmid#86081PurposeA mammalian expression plasmid encoding Transportin-1 (TNPO1) with N-terminus double Myc-tag.DepositorInsertTransportin-1 (TNPO1 Human)
UseTagsMycExpressionMammalianMutationPromoterT7Available sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
TCRalpha_E2A_SP-SNAPf-TCRbeta_P2A_CD3e_T2A_CD3zeta
Plasmid#89347Purpose2nd generation lentiviral vector which expresses SNAP tagged TCRbeta and other TCR subunits (Jurkat alleles) separated via 2A sequencesDepositorInsertJurkat TCRalpha/beta alleles and CD3e and CD3z
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceMay 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-N_NUP214:1-2090
Plasmid#211220PurposeMammalian expression of full-length human NUP214. N-terminal HiBiT tag.DepositorInsertNUP214:M1-S2090 (NUP214 Human)
UseTagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationP574S natural variant (VAR_045691). S1872N annot…PromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAH123
Plasmid#66077PurposeThe pAH123 plasmid [Haldimann2001] is the phi80 helper plasmid necessary for the genome integration of the burden monitor phi80 versionDepositorInsertphi80 integrase
UseTagsExpressionMutationPromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
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AAV2-ITR-pEFS-NLS_hfCas13d(RfxCas13d_N2V8)_FLAG_NLS-pA-U6-DR-Pcsk9-sg5-DR-Pcsk9-sg6-DR-AAV2-ITR
Plasmid#191795Purposevector for encoding and AAV packaging a human codon-optimized High-fidelity RfxCas13d (hfCas13d) driven by EFS promoter, Pcsk9-target guide RNAs compatible with RfxCas13d driven by hU6DepositorInserthumanized hfCas13d
UseAAV and CRISPRTagsExpressionMammalianMutationA134V, A140V, A141V, A143VPromoterEFS, hU6Available sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only