-
Plasmid#112502PurposeExpresses 10xHis-Cas14a1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoterDepositorInsertCas14a1
UseCRISPRTags6xHis-TEVExpressionBacterialMutationPromoterTetAvailable sinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWJ68
Plasmid#46571PurposeSame as pdCas9, but with the w-dCas9 fusion (w is fused at the N-terminal end of dCas9)DepositorInsertstracrRNA
w-dcas9
CRISPR array
UseCRISPRTagsrpoZExpressionBacterialMutationD10A, H840APromoterAvailable sinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMH-MBP
Plasmid#101768PurposeGateway-compatible destination vector for bacterial expression of N-terminal MBP-tag fusion proteinDepositorTypeEmpty backboneUseTagsMBPExpressionBacterialMutationPromoterAvailable sinceOct. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAP24
Plasmid#70190PurposeModular vector for secreted luciferase reporter expressionDepositorInsertsLucopt
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPtetAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR-221-P3-P4
Plasmid#186351PurposeGateway donor vector with AttP3 AttP4 recombination sitesDepositorTypeEmpty backboneUseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBad33_ISTfu1_reRNA
Plasmid#205355PurposeBacterial expression of ISTfu1 TnpB and its reRNADepositorInsertISTfu1 TnpB
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHL 1903
Plasmid#53020PurposeAmpR + fimS::gfpAAV::Asp terminator + CamR + ColE1DepositorInsertGFP
UseSynthetic BiologyTagsAAV, Asp terminator, and fimSExpressionBacterialMutationPromoterfimSAvailable sinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBad33_ISMmu1_reRNA
Plasmid#205349PurposeBacterial expression of ISMmu1 TnpB and its reRNADepositorInsertISMmu1 TnpB
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMS13: pTarget(AvCAST)
Plasmid#168146PurposeTarget plasmid containing AvPSP1 and attachement site for AvCAST.DepositorInsertsPAM for AvCAST + AvPSP1
glmS
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGAPi
Plasmid#127547PurposeGateway-based RNAi vector to clone target gene for silencing in tandem with APT transcript segmentDepositorTypeEmpty backboneUseRNAiTagsExpressionMutationPromoterUbiquitinAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL2_171
Plasmid#199103PurposeLevel 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporterDepositorInsertpWKS1 ORI plus NGS barcode 17, gentamicin resistance cassette, mScarlet-I cassette, oriT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQCXIH CMV/TO DEST (w382-1)
Plasmid#17394PurposeRetroviral Gateway destination vector, Expression, CMV/TO promoter, HygroDepositorTypeEmpty backboneUseRetroviral; Destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pJK506
Plasmid#155382PurposedCas12a (D917A) + PA4-mVenus. (removed crRNAs)DepositorInsertsdCas12a (F. novicida)
PA4-mVenus
UseCRISPRTagsExpressionBacterialMutationD917A (nuclease-deactivating)PromoterAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRU53
Plasmid#167679PurposeFinal destination vector for single Gateway LR reactionDepositorInsertpEC1.2::spCas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
FU-MAP2-Gateway
Plasmid#43915DepositorTypeEmpty backboneUseLentiviral; Gateway destination vectorTagsV5 and non-standard His tag (HHRHHH)ExpressionMammalianMutationPromoterHuman MAP2 promoterAvailable sinceMay 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046979
Plasmid#145611PurposeBacterial Expression plasmid for SARS-CoV-2 nsp7DepositorInsertSARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
-
pRU54
Plasmid#167680PurposeFinal destination vector for single Gateway LR reactionDepositorInsertpEC1.2::spCas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTECH-MbPylRS(IPYE)
Plasmid#104072PurposeExpression plasmid encoding MbPylRS(IPYE) and pylTDepositorInsertPlpp MbPylRS(IPYE); PproK pyl
UseTagsExpressionBacterialMutationContains activating mutations V31I, T56P, H62Y, A…PromoterPlpp and PproKAvailable sinceDec. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCK01_PalkS_alkS
Plasmid#166502PurposeExpression of AlkS transcriptional regulator with L401G mutation. Sensor input plasmid for detection of n-butanol and branched-chain higher alcohols.DepositorInsertalkS
UseSynthetic BiologyTagsExpressionBacterialMutationL401GPromoterPalkSAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only