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Showing: 3701 - 3720 of 18558 results

  1. CRISPseq-mCherry-backbone

    Plasmid
    #85708
    Purpose
    Backbone for CRISPR/Cas9 screening with single cell RNA-seq. Lentiviral plasmid for cloning of gRNAs and Unique Guide Index (UGI), with an mCherry fluorescent marker. Does NOT include Cas9.
    Depositor
    Ido Amit
    Article
    Jaitin et al Cell. 2016 Dec 15;167(7):1883-1896.e15.
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    85708-plasmid-map-sequence-id-170481

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  3. RS35075_pMQ30_KO_Kan

    Plasmid
    #185390
    Purpose
    Non-replicating vector used to create markerless deletion of RR42_RS35075 in C. basilensis 4G11
    Depositor
    Adam Arkin
    Article
    Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1
    Inserts
    RR42_RS35075 upstream flanking homology region
    RR42_RS35075 downstream flanking homology region
    nptII kanamycin resistance marker
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    185390-plasmid-map-sequence-id-371206

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  4. Direct-seq_lentiGuide-Puro-Loop2-8A8G

    Plasmid
    #157983
    Purpose
    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.
    Depositor
    Lijia Ma
    Article
    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:
    Insert
    S. pyogenes sgRNA cassette (Loop2-8A8G)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    May 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    157983-plasmid-map-sequence-id-332422

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  6. Direct-seq_lentiGuide-Puro-Tail-8A8G

    Plasmid
    #157981
    Purpose
    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.
    Depositor
    Lijia Ma
    Article
    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:
    Insert
    S. pyogenes sgRNA cassette (Tail-8A8G)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    Aug. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    157981-plasmid-map-sequence-id-309444

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  7. pOCC94

    Plasmid
    #118912
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal, N-terminal MBP, cleavable with 3C protease, and C-terminal eGFP-HIS6
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HBMss-MBP-3C-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
    Use
    Tags
    MBP, cleavable with 3C protease and eGFP-HIS6
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118912-plasmid-map-sequence-id-232258

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  8. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_cpaA- Pconstitutive-sgRNA(Sth3)_blaA

    Plasmid
    #133347
    Purpose
    expression of two sgRNA from Streptococcus thermophilus #3 each express from its own constitutive promoter; here first one targets cpaA and second one targets blaA (from Caulobacter crescentus)
    Depositor
    Michael Laub
    Article
    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:
    Insert
    sgRNA_cpaA and sgRNA_blaA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    constitutive
    Available since
    Jan. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    133347-plasmid-map-sequence-id-269540

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  15. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_blaA- Pconstitutive-sgRNA(Sth3)_cpaA

    Plasmid
    #133348
    Purpose
    expression of two sgRNA from Streptococcus thermophilus #3 each express from its own constitutive promoter; here first one targets blaA and second one targets cpaA (from Caulobacter crescentus)
    Depositor
    Michael Laub
    Article
    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:
    Insert
    sgRNA_blaA and sgRNA_cpaA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    constitutive
    Available since
    Jan. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    133348-plasmid-map-sequence-id-267409

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  19. pLVX-TetOne-Puro-EcSTH-FLAG

    Plasmid
    #218628
    Purpose
    This plasmid contains human codon optimized sequence of EcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells.
    Depositor
    Valentin Cracan
    Article
    Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038
    Insert
    EcSTH
    Use
    Lentiviral and Synthetic Biology
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    218628-plasmid-map-sequence-id-432188

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  20. pLVX-TetOne-Puro-mitoEcSTH-FLAG

    Plasmid
    #218629
    Purpose
    This plasmid contains human codon optimized sequence of mitoEcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells.
    Depositor
    Valentin Cracan
    Article
    Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038
    Insert
    mitoEcSTH
    Use
    Lentiviral and Synthetic Biology
    Tags
    FLAG and MTS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    218629-plasmid-map-sequence-id-432199

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Showing: 3701 - 3720 of 18558 results