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Showing: 3701 - 3720 of 10447 results

  1. pPMQAK1-Ptrc-[E*](104)-Cas9-B0015-lacZ-PnrsB-mazF-LVA

    Plasmid
    #190790
    Purpose
    [E*](104) construct. Theophylline inducible Cas9 (S. pyogenes) expression. Two BsaI-sites allow for simultaneous insertion of sgRNA and donor DNA. Includes a nickel-inducible curing system.
    Depositor
    Paul Hudson
    Article
    Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102
    Inserts
    Cas9
    mazF-LVA
    Use
    CRISPR and Synthetic Biology
    Tags
    LVA
    Expression
    Bacterial
    Mutation
    silent mutations of BpiI-sites in original Cas9 g…
    Promoter
    PnrsB and Ptrc-[E*](104)
    Availability
    Academic Institutions and Nonprofits only
    190790-plasmid-map-sequence-id-384200

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  5. pJEC726

    Plasmid
    #190814
    Purpose
    CRISPRi backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.
    Depositor
    James Chappell
    Article
    Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    dCas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    S. pyogenes dCas9 codon optimized for Streptomyce…
    Promoter
    SP30
    Availability
    Academic Institutions and Nonprofits only
    190814-plasmid-map-sequence-id-373712

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  6. pJEC819

    Plasmid
    #190815
    Purpose
    CRISPRa backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.
    Depositor
    James Chappell
    Article
    Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    dCas9-XTEN-rpoA
    Use
    CRISPR and Synthetic Biology
    Tags
    XTEN (linker), followed by the N-terminal domain …
    Expression
    Mutation
    Promoter
    SP30
    Availability
    Academic Institutions and Nonprofits only
    190815-plasmid-map-sequence-id-373717

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  7. pSilencer2.1-U6-T1

    Plasmid
    #190825
    Purpose
    For mammalian expression of T1, the RNA aptamer of ncOGT. Expression is driven by a U6 promoter.
    Depositor
    Gerald Hart
    Article
    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.
    Insert
    T1 (an RNA aptamer of O-GlcNAc Transferase)
    Use
    Affinity Reagent/ Antibody and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Industry, Academic Institutions, and Nonprofits
    190825-plasmid-map-sequence-id-379207

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  9. pSilencer2.1-U6-T1-U6-AP3

    Plasmid
    #190828
    Purpose
    For mammalian expression of T1 and AP3, the RNA aptamers of ncOGT and GFP, respectively. Expression of each aptamer is driven by a U6 promoter.
    Depositor
    Gerald Hart
    Article
    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.
    Inserts
    T1 (an RNA aptamer of OGT)
    AP3 (an RNA aptamer of GFP)
    Use
    Affinity Reagent/ Antibody and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Industry, Academic Institutions, and Nonprofits
    190828-plasmid-map-sequence-id-379201

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  10. pSilencer2.1-U6-NL1F30

    Plasmid
    #190829
    Purpose
    For mammalian expression of NL1F30, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a flexible linker.
    Depositor
    Gerald Hart
    Article
    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.
    Insert
    NL1F30 (A Dual-Specificity aptamer targeting OGT and GFP)
    Use
    Affinity Reagent/ Antibody and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Industry, Academic Institutions, and Nonprofits
    190829-plasmid-map-sequence-id-379188

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  11. pSilencer2.1-U6-NL1F35

    Plasmid
    #190830
    Purpose
    For mammalian expression of NL1F35, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a flexible linker.
    Depositor
    Gerald Hart
    Article
    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.
    Insert
    NL1F35 (A Dual-Specificity aptamer targeting OGT and GFP)
    Use
    Affinity Reagent/ Antibody and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Industry, Academic Institutions, and Nonprofits
    190830-plasmid-map-sequence-id-379202

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  12. pSilencer2.1-U6-NL1F50

    Plasmid
    #190831
    Purpose
    For mammalian expression of NL1F50, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a flexible linker.
    Depositor
    Gerald Hart
    Article
    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.
    Insert
    NL1F50 (A Dual-Specificity aptamer targeting OGT and GFP)
    Use
    Affinity Reagent/ Antibody and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Industry, Academic Institutions, and Nonprofits
    190831-plasmid-map-sequence-id-379228

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  13. pSilencer2.1-U6-3JB1F

    Plasmid
    #190832
    Purpose
    For mammalian expression of 3JB1F, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.
    Depositor
    Gerald Hart
    Article
    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.
    Insert
    3JB1F (A Dual-Specificity aptamer targeting OGT and GFP)
    Use
    Affinity Reagent/ Antibody and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Industry, Academic Institutions, and Nonprofits
    190832-plasmid-map-sequence-id-379233

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  14. pSilencer2.1-U6-3JB1F+2

    Plasmid
    #190833
    Purpose
    For mammalian expression of 3JB1F+2, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.
    Depositor
    Gerald Hart
    Article
    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.
    Insert
    3JB1F_T1+2bp (A Dual-Specificity aptamer targeting OGT and GFP)
    Use
    Affinity Reagent/ Antibody and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Industry, Academic Institutions, and Nonprofits
    190833-plasmid-map-sequence-id-379189

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  15. pSilencer2.1-U6-3JB1F+4

    Plasmid
    #190834
    Purpose
    For mammalian expression of 3JB1F+4, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.
    Depositor
    Gerald Hart
    Article
    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.
    Insert
    3JB1F_T1+4bp (A Dual-Specificity aptamer targeting OGT and GFP)
    Use
    Affinity Reagent/ Antibody and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Industry, Academic Institutions, and Nonprofits
    190834-plasmid-map-sequence-id-379203

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  16. pSilencer2.1-U6-3JB1F+6

    Plasmid
    #190835
    Purpose
    For mammalian expression of 3JB1F+6, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.
    Depositor
    Gerald Hart
    Article
    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.
    Insert
    3JB1F_T1+6bp (A Dual-Specificity aptamer targeting OGT and GFP)
    Use
    Affinity Reagent/ Antibody and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Industry, Academic Institutions, and Nonprofits
    190835-plasmid-map-sequence-id-379214

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  17. pSilencer2.1-U6-3JB1F+8

    Plasmid
    #190836
    Purpose
    For mammalian expression of 3JB1F+8, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.
    Depositor
    Gerald Hart
    Article
    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.
    Insert
    3JB1F_T1+8bp (A Dual-Specificity aptamer targeting OGT and GFP)
    Use
    Affinity Reagent/ Antibody and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Industry, Academic Institutions, and Nonprofits
    190836-plasmid-map-sequence-id-379211

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  18. pSilencer2.1-U6-3JB1F+10

    Plasmid
    #190837
    Purpose
    For mammalian expression of 3JB1F+10, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.
    Depositor
    Gerald Hart
    Article
    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.
    Insert
    3JB1F_T1+10bp (A Dual-Specificity aptamer targeting OGT and GFP)
    Use
    Affinity Reagent/ Antibody and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Industry, Academic Institutions, and Nonprofits
    190837-plasmid-map-sequence-id-379234

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  19. pSilencer2.1-U6-3JB1F+12

    Plasmid
    #190838
    Purpose
    For mammalian expression of 3JB1F+12, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.
    Depositor
    Gerald Hart
    Article
    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.
    Insert
    3JB1F_T1+12bp (A Dual-Specificity aptamer targeting OGT and GFP)
    Use
    Affinity Reagent/ Antibody and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Industry, Academic Institutions, and Nonprofits
    190838-plasmid-map-sequence-id-379192

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  20. pSilencer2.1-U6-3JB1R

    Plasmid
    #190839
    Purpose
    For mammalian expression of 3JB1R, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.
    Depositor
    Gerald Hart
    Article
    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.
    Insert
    3JB1R (A Dual-Specificity aptamer targeting OGT and GFP)
    Use
    Affinity Reagent/ Antibody and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Industry, Academic Institutions, and Nonprofits
    190839-plasmid-map-sequence-id-379216

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Showing: 3701 - 3720 of 10447 results