Showing: 3701 - 3720 of 12967 results
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Plasmid#86358PurposeEncodes gRNA for 3' target of human CEBPADepositorInsertgRNA against CEBPA (CEBPA Human)
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
PX458_CEBPA_2
Plasmid#86359PurposeEncodes gRNA for 3' target of human CEBPADepositorInsertgRNA against CEBPA (CEBPA Human)
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
PX458_ZFP1_iso1_1
Plasmid#86360PurposeEncodes gRNA for 3' target of human ZFP1_iso1DepositorInsertgRNA against ZFP1_iso1 (ZFP1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
PX458_ZFP1_iso1_2
Plasmid#86361PurposeEncodes gRNA for 3' target of human ZFP1_iso1DepositorInsertgRNA against ZFP1_iso1 (ZFP1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
PX458_SP5_1
Plasmid#86362PurposeEncodes gRNA for 3' target of human SP5DepositorInsertgRNA against SP5
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
PX458_SP5_2
Plasmid#86363PurposeEncodes gRNA for 3' target of human SP5DepositorInsertgRNA against SP5
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
PX458_RFXANK_1
Plasmid#86364PurposeEncodes gRNA for 3' target of human RFXANKDepositorInsertgRNA against RFXANK (RFXANK Human)
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
PX458_RFXANK_2
Plasmid#86365PurposeEncodes gRNA for 3' target of human RFXANKDepositorInsertgRNA against RFXANK (RFXANK Human)
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAc5 HA3-eDHFR-T2A-puro
Plasmid#86395Purposefor C-terminal tagging with eDHFR trimethoprim regulated degron with puro selectionDepositorInsertHA3 eDHFR T2A puro
UseCRISPRTagsHA3 eDHFR T2A puroExpressionMutationR12Y, G67S and Y100I mutations in E coli DHFRPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAc5 HA3-eDHFR-T2A-neo
Plasmid#86396Purposefor C-terminal tagging with eDHFR trimethoprim regulated degron with neo selectionDepositorInsertHA3 eDHFR T2A neo
UseCRISPRTagsHA3 eDHFR T2A neoExpressionMutationR12Y, G67S and Y100I mutations in E coli DHFRPromoterAvailabilityAcademic Institutions and Nonprofits only -
pKIR1.0
Plasmid#86409PurposeCRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.DepositorInserthuman-codon-optimized SpCas9
UseCRISPRTagsFLAG and NLSExpressionMutationPromoterAtRPS5AAvailabilityAcademic Institutions and Nonprofits only -
MSCV-pU6-(BbsI)-CcdB-(BbsI)-Pgk-Puro-T2A-BFP
Plasmid#86457PurposeMouse stem cell retroviral vector including a puromycin resistance and BFP gene. sgRNA targets can be cloned in between the BbsI sitesDepositorInsertBFP
UseCRISPRTagsExpressionMammalianMutationPromoterPgkAvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_plasmid_donor_RD
Plasmid#86551PurposeHomology-directed repair (HDR) donor plasmid with homology arms to ATP1A1 to introduce the Q118R and N129D (RD) mutations conferring cellular resistance to ouabainDepositorInsertATP1A1 (ATP1A1 Human)
UseCRISPR and TALEN; Co-selection via hdr using ouab…TagsExpressionMammalianMutationQ118R, N129DPromoterAvailabilityAcademic Institutions and Nonprofits only -
pFH6
Plasmid#86555PurposeSubcloning of any sgRNA via BbsI sites. Note that there is an improved version of this plasmid (Addgene 105866) that results in up to 10x greater efficiencyDepositorInsertU6-26p::sgRNA scaffold
UseCRISPR; SubcloningTagsExpressionMutationPromoterArabidopsis U6-26 promoterAvailabilityAcademic Institutions and Nonprofits only -
pUB-Cas9
Plasmid#86556PurposeFor ubiquitous expression of Cas9 in planta. sgRNA can be introduced as described in Hahn et al. (2017)DepositorInsertCas9
UseCRISPRTagsExpressionPlantMutationCas9 is codon optimized for C. reinhardtiiPromoterUBIQUITIN10AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9(1.1)_No_FLAG_ATP1A1_G2
Plasmid#86610PurposeExpresses the ATP1A1 G2 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 exon 4. Px330-like plasmidDepositorInsertATP1A1 G2 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
UseCRISPR; Co-selection via nhej using ouabainTagsExpressionMammalianMutationK848A, K1003A, & R1060APromoterCBhAvailabilityAcademic Institutions and Nonprofits only -
eSpCas9(1.1)_No_FLAG_ATP1A1_G3
Plasmid#86611PurposeExpresses the ATP1A1 G3 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 intron 4. Px330-like plasmidDepositorInsertATP1A1 G3 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
UseCRISPR; Co-selection via hdr using ouabainTagsExpressionMammalianMutationK848A, K1003A, & R1060APromoterCBhAvailabilityAcademic Institutions and Nonprofits only -
eSpCas9(1.1)_No_FLAG_ATP1A1_G2_Dual_sgRNA
Plasmid#86612PurposeVector for tandem expression of ATP1A1 G2 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmidDepositorInsertATP1A1 G2 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
UseCRISPR; Co-selection via nhej using ouabainTagsExpressionMammalianMutationK848A, K1003A, & R1060APromoterCBhAvailabilityAcademic Institutions and Nonprofits only -
eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA
Plasmid#86613PurposeVector for tandem expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmidDepositorInsertATP1A1 G3 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
UseCRISPR; Co-selection via hdr using ouabainTagsExpressionMammalianMutationK848A, K1003A, & R1060APromoterCBhAvailabilityAcademic Institutions and Nonprofits only -
pY036_ATP1A1_G3
Plasmid#86617PurposeExpresses the ATP1A1 G3 crRNA in combination with AsCpf1-3xHA to target ATP1A1 exon 4. U6-crRNA(ATP1A1 G3)-CBh-AsCpf1DepositorInsertATP1A1 G3 crRNA + AsCpf1-3xHA
UseCRISPR; Co-selection via nhej using ouabainTags3xHAExpressionMammalianMutationPromoterCBhAvailabilityAcademic Institutions and Nonprofits only
Showing: 3701 - 3720 of 12967 results