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Plasmid#192094PurposeHuman K202R short-form UBASH3A in Gateway pDONR221 (for N-terminal tag)DepositorInsertUBASH3A (UBASH3A Human)
UseTagsExpressionMammalianMutationK202RPromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-WT-short-UBASH3A-Ctag
Plasmid#192099PurposeHuman wild-type short-form UBASH3A in Gateway pDONR221 (for C-terminal tag)DepositorInsertUBASH3A (UBASH3A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-WT-short-UBASH3A-Ntag
Plasmid#192100PurposeHuman wild-type short-form UBASH3A in Gateway pDONR221 (for N-terminal tag)DepositorInsertUBASH3A (UBASH3A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENT L1-Kozak-ERK-KTR-L2
Plasmid#186353PurposeEntry clone with ORF encoding ERK KTR flanked by Gateway recombination sequences. No Stop sequence: to be recombined into a destination vector with a C-terminal protein tag.DepositorInsertERK Kinase Translocation Reporter (MAPK1 Synthetic, Human)
UseProtein expressionTagsExpressionMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMLS715
Plasmid#188887PurposeMultisite Gateway [4-3] Destination vector; lox2272-flanked Cbr-unc-119 marker; homology to 5' miniMos ArmDepositorTypeEmpty backboneUseMultisite gateway [4-3] destination vectorTagsExpressionMutationPromoterAvailable sinceAug. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
p3e tdTomato
Plasmid#188700PurposeGateway 3' element encoding tdTomatoDepositorInserttdTomato
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
C232-E08: attB2r-mTFP1-attB3
Plasmid#162904PurposeGateway attB2r/attB3 entry clone for construction of C-terminal mTFP1 fusion proteinsDepositorInsertmonomeric Teal fluorescent protein
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pME-Ruby2-NS
Plasmid#166569PurposeMultisite Gateway middle entry clone encoding mRuby2. Parton lab clone DJADepositorInsertRuby2
UseMultisite gateway entry cloneTagsExpressionMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
C453-E18: attB4-polyhedrinp>-attB5
Plasmid#162977PurposeGateway attB4/attB5 entry clone for baculovirus expression using the AcMNPV polyhedrin promoterDepositorInsertAcMNPV polyhedrin promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
C453-E21: attB4-FerHp>-attB5
Plasmid#162978PurposeGateway attB4/attB5 entry clone for mammalian expression using the human EF1alpha promoterDepositorInserthuman Ferritin heavy chain promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
C413-E38: attB4-T7p>-attB1r
Plasmid#162929PurposeGateway attB4/attB1r entry clone for bacterial expression using the bacteriophage T7 promoterDepositorInsertbacteriophage T7 promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
C453-E26: attB4-T7p>-attB5
Plasmid#162982PurposeGateway attB4/attB1r entry clone for bacterial expression using the bacteriophage T7 promoterDepositorInsertbacteriophage T7 promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
C512-E01: attB5r-meGFP-nostop-attB1r
Plasmid#162940PurposeGateway attB5r/attB1r entry clone for generation of N-terminal eGFP fusion proteinsDepositorInsertmonomeric enhanced Green fluorescent protein
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
C511-E15: attB5r-ffluc2-nostop-attB1r
Plasmid#162939PurposeGateway attB5r/attB1r entry clone for generation of N-terminal Nanoluciferase fusion proteinsDepositorInsertFirefly luciferase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
C413-E40: attB4-EFSp>-attB1r
Plasmid#162928PurposeGateway attB4/attB1r entry clone for mammalian expression using the short EF1 promoterDepositorInsertshort form of human EF1 alpha promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
C413-E34: attB4-mCMVp>-attB1r
Plasmid#162926PurposeGateway attB4/attB1r entry clone for mammalian expression using the minimal CMV promoterDepositorInsertminimal CMV IE1 promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
C238-E07: attB2r-SV40polyA-attB3
Plasmid#162917PurposeGateway attB2r/attB3 entry clone containing a polyA sequence for transcription terminationDepositorInsertSV40 polyadenylation sequence
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
C234-E24: attB2r-Strep2-attB3
Plasmid#162913PurposeGateway attB2r/attB3 entry clone for construction of C-terminal Strep2 tagged fusion proteinsDepositorInsertStrep2 epitope tag
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
C232-E05: attB2r-meGFP-attB3
Plasmid#162902PurposeGateway attB2r/attB3 entry clone for construction of C-terminal eGFP fusion proteinsDepositorInsertmonomeric enhanced Green fluorescent protein
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
C231-E02: attB2r-NanoLuc-attB3
Plasmid#162901PurposeGateway attB2r/attB3 entry clone for construction of C-terminal NanoLuc fusion proteinsDepositorInsertNanoluciferase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only