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Plasmid#170536PurposeExpresses PTPIP51(1-470) in human HeLa cells after transfectionDepositorInsertProtein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
UseTagsFLAG tagExpressionMammalianMutationPromoterCAG promoterAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-FLAG-Ku70
Plasmid#46957PurposePlasmid for cloning and epression of GFP-FLAG tagged human Ku70 (N-terminal tag). Confers resistance to G418.DepositorInsertKu70 (XRCC6 Human)
UseTagsGFP-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
TFORF2475
Plasmid#144337PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZFHX3 (ZFHX3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
p3E_BAG3wt-eGFP
Plasmid#98180PurposeCodes for full length, GFP tagged, human wildtype Bag3DepositorInsertBAG3wt-eGFP (BAG3 Human)
UseGateway vectorTagsGFPExpressionMutationPromoterAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
Lamp1-mCherry-FRB
Plasmid#186576PurposeExpression of mCherry-FRB at the outer surface of the lysosome using LAMP1DepositorInsertLAMP1 (LAMP1 Human)
UseTagsmCherry-FRBExpressionMammalianMutationPromoterCMVAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
7xSMAD_RE::FLuc
Plasmid#124531PurposeSMAD2 FLuc luciferase reporterDepositorInsertSMAD FLuc reporter
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
R777-E285 Hs.ROCK2
Plasmid#70569PurposeGateway ORF clone of human ROCK2 [NM_004850.3] with stop codon (for native or N-terminal fusions)DepositorInsertROCK2 (ROCK2 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFLAG-CMV2 ACAP1
Plasmid#15697DepositorInsertACAP1 (ACAP1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCMV6M-PAK1 T423E
Plasmid#12208DepositorInsertPak1 (PAK1 Human)
UseTagsMycExpressionMammalianMutationActivated Pak1: T423EPromoterAvailable sinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-p300 Core (D1399Y)
Plasmid#61358Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9; D1399Y mutation i…PromoterCMVAvailable sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
FLAG-FOXO3a WT
Plasmid#8360DepositorInsertFOXO3a (FOXO3 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
PJ-DEAD
Plasmid#38002DepositorUseTagsmRFPExpressionMammalianMutationAsp 1263 mutated to Ala and Cys 779 mutated to SerPromoterAvailable sinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-ARAP3
Plasmid#39484DepositorInsertARAP3 (ARAP3 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
mCherry-NES-SspB-LARG-DH-P2A-iLIDcaax
Plasmid#173870PurposeMammalian expression of mCherry-NES-SspB-LARG-DH-P2A-iLIDcaax (opto-RhoA)DepositorInsertmCherry-NES-SspB-LARG-DH-P2A-iLIDcaax
UseTagsC-terminal CAAX-box and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
Kif1bβ-pcDNA3
Plasmid#22534DepositorInsertKif1bβ (KIF1B Human)
UseTagsFLAGExpressionMammalianMutationwt cDNAPromoterAvailable sinceDec. 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-GRAB_ACh3.0
Plasmid#121923Purposeexpress the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0 in a cre-dependent mannerDepositorInsertGPCR activation based ACh sensor GRAB-ACh3.0
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceJan. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-VAMP2-IRES-Puromycin
Plasmid#134661PurposeExpresses EGFP-tagged synaptic vesicle proteinDepositorInsertvesicle-associated membrane protein 2 (VAMP2 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pInducer20-Blast-CDT1-HA
Plasmid#109335Purposevirus packaged, doxycyline inducible expression of Cdt1DepositorInsertCdt1 (CDT1 Human)
UseLentiviral; Inducible expressionTagsHA, His, StrepExpressionMammalianMutationPromoterAvailable sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_ADAR2DD_E488Q_Ago2
Plasmid#205599Purposeexpresses human ADAR2 deaminase domain (E488Q hyperactive mutant) fused with human Ago2 in mammalian cellsDepositorInserthAgo2 (AGO2 Human)
UseTagsADAR2DD_E488Q (hyperactive mutant)ExpressionMammalianMutationPromoterAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pInducer20 Cyclin E1
Plasmid#109348Purposevirus packaged, doxycyline inducible expression of Cyclin E1DepositorInsertCyclin E1 (CCNE1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only