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pBEN276

Plasmid

#69150

Purpose

Integration of luciferase operon in Tn7 insertion site of Gram negative bacteria

Depositor

Pierre Germon

Article

Howe et al BMC Microbiol. 2010 Jul 23;10:197. doi:

Insert

luxCDABE

Use

Luciferase

Tags

Expression

Mutation

Promoter

frr

Available since

Dec. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-EGFP

Plasmid

#89875

Purpose

EGFP reporter vector of iTango system for AAV production

Depositor

Hyungbae Kwon

Article

Lee et al Nat Methods. 2017 May;14(5):495-503. doi

Insert

tetO-EGFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

tetO

Available since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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pYPQ141-ZmUbi-RZ-Lb

Plasmid

#86197

Purpose

LbCpf1 Gateway gRNA entry plasmid using Zea mays Ubi promoter and ribozyme processing

Depositor

Yiping Qi

Article

Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

Insert

gRNA cloning site

Use

CRISPR

Tags

Expression

Mutation

Promoter

Maize ubiquitin 1

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG vGlut1 mCherry

Plasmid

#105010

Purpose

Excitatory presynaptic marker

Depositor

Franck Polleux

Article

Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

Insert

SLC17A7

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_SPOP_WT

Plasmid

#81856

Purpose

Gateway Donor vector containing SPOP ,part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, David Root, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SPOP (SPOP Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCA24N-p50-ligase

Plasmid

#87742

Purpose

IPTG-inducible expression of p50-ligase fusion for protein purification

Depositor

Wayne Patrick

Article

Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

Insert

T4 DNA ligase (30 )

Use

Tags

6xHis and human p50/NF-kB (amino acids 40-366)

Expression

Bacterial

Mutation

Promoter

T5-lac

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-VSVM

Plasmid

#64086

Purpose

Expression vector for Vesicular Stomatitis Virus Indiana strain matrix gene

Depositor

Ian Wickersham

Insert

VSV matrix protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA

Plasmid

#61592

Purpose

A human codon-optimized Cas9 from Staphylococcus aureus (SaCas9).

Depositor

Feng Zhang

Article

Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

Insert

hSaCas9

Use

AAV and CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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SpyTag003-mKate2

Plasmid

#133452

Purpose

Expresses SpyTag003-mKate2 (a far-red fluorescent protein) in bacterial cytoplasm

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyTag003-mKate2

Use

Tags

GSGSGS linker and His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pEIGW roGFP2-Orp1

Plasmid

#64993

Purpose

Mammalian expression of cytosolic roGFP2-Orp1 (lentiviral vector)

Depositor

Tobias Dick

Article

Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

Insert

Orp1 (HYR1 Budding Yeast)

Use

Lentiviral

Tags

roGFP2

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-Giantin

Plasmid

#98880

Purpose

In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv 160374

Insert

Giantin

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-FLAG-XRCC4

Plasmid

#46959

Purpose

Plasmid for expression of GFP-FLAG tagged human XRCC4 isoform 2 (N-terminal tag). Confers resistance to G418.

Depositor

Steve Jackson

Article

Britton et al J Cell Biol. 2013 Jul 29.

Insert

XRCC4 (XRCC4 Human)

Use

Tags

GFP-2xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA

Plasmid

#154868

Purpose

Human synapsin-1 promoter; Flp-dependent hM3D(Gq) DREADD-mCherry

Depositor

Ulrik Gether

Has Service

AAV Retrograde and AAV8

Insert

hM3D(Gq)-mCherry

Use

AAV; Flp-frt

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Human Synapsin-1

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-EGFPL10a

Plasmid

#98747

Purpose

Cre-dependently expresses the fusion protein EGFPL10a

Depositor

Nathaniel Heintz, Eric Schmidt, Alexander Nectow

Article

Nectow et al Cell Rep. 2017 Apr 18;19(3):655-667. doi

Has Service

AAV Retrograde and AAV5

Insert

EGFPL10a

Use

AAV and Cre/Lox

Tags

GFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pRSET Nucleolin

Plasmid

#13037

Depositor

Peter Newburger

Article

Wu et al J Cell Biochem. 2000 Apr . 77(3):507-16.

Insert

Nucleolin (NCL Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pICH47761

Plasmid

#48003

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pLentiRNAGuide_002 - hU6-RfxCas13d-DR-BsmBI-EFS-Puro-WPRE

Plasmid

#138151

Purpose

For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

Depositor

Neville Sanjana

Article

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

hU6_Rfxcas13d-DR_BsmBI

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090

Plasmid

#103871

Purpose

dPspCas13b-ADAR2DD(E488Q/T375G) fusion that can be used to selectively edit adenosine to inosine in RNA when used in conjuction with a guide RNA. Cas13b is truncated to allow for expression by AAV

Depositor

Feng Zhang

Article

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Inserts

PspCas13b
huADAR2DD (ADARB1 Human)

Use

CRISPR

Tags

GS and HIV NES

Expression

Mammalian

Mutation

Changed histidne 133 to alanine and histidine 105…

Promoter

Available since

Nov. 28, 2017

Availability

Academic Institutions and Nonprofits only

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human EB1 in pEGFP N1 to express EB1-GFP (JB131)

Plasmid

#39299

Depositor

Jennifer Tirnauer, Tim Mitchison

Insert

EB1 (MAPRE1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RAB-EKARev

Plasmid

#118474

Purpose

ddRFP-based single-color biosensor for monitoring ERK activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

RAB-EKARev

Use

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 30, 2019

Availability

Academic Institutions and Nonprofits only

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