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Plasmid#170347PurposemT2Del_EPACdDEPCD_tdBlackcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.DepositorInsertmT2Del_EPACdDEPCD_tdBlackcp173Ven(ST) (RAPGEF3 Human)
UseTagsExpressionMammalianMutationDeletion of DEP domain, Catalytically dead T781A …PromoterCMVAvailable sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKK-BI16-ORF1-3C-mRuby3_ORF2-TEV-mClover3
Plasmid#105802PurposeConcomitant expression of two proteins. One protein expressed with mRuby3, cleavable by 3C; second protein expressed with mClover3, cleavable by TEV protease; tags positions: C-termini.DepositorTypeEmpty backboneUseFlp-in competentTagsORF1: 3C-mRuby3; ORF2: TEV-mClover3ExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
plenti-ef1a-dCasRx-FTO-HA-T2A-BSD
Plasmid#177120PurposedCasRx mediated RNA tethering of FTODepositorInsertdCasRx-FTO
UseLentiviralTagsHA and SV40 NLSExpressionMutationdCasRX contains R239A/H244A/R858A/H863A mutations…PromoterEf1aAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-PGK-Chst3
Plasmid#193024PurposeAAV vector plasmid expressing mouse chondroitin 6-sulfotransferase (Chst3) under the mouse phosphoglycerate kinase (PGK) promoterDepositorInsertChst3 (Chst3 Mouse)
UseAAVTags3xFLAG and myc-tagExpressionMammalianMutationPromoterPGKAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
FGFR1_HUMAN_D0
Plasmid#79719PurposeThis plasmid encodes the kinase domain of FGFR1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertFGFR1 (FGFR1 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR-IDR1-mCherry-Cry2
Plasmid#177125PurposeExpress IDR1 fragment of YTHDC1 with mCherry and CRY2 tag for Optodroplet AssayDepositorInsertYTHDC1 (IDR1 domain) (YTHDC1 Human)
UseTagsmCherry-CRY2ExpressionMammalianMutationIDR1 domain onlyPromoterSFFVAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAC1-V16-Luc
Plasmid#122034PurposeAAV vector, pTet bidi (bidirectional tetracycline-responsive promoter) driving Tet-dependent rtTA and Luciferase expressionDepositorInsertLuciferase
UseAAVTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR207 SARS-CoV-2 NSP1_nostop
Plasmid#149304PurposeGateway-compatible Entry vector, with insert of NSP1 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.DepositorInsertNSP1 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceMay 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR207 SARS-CoV-2 E_nostop
Plasmid#149321PurposeGateway-compatible Entry vector, with insert of the envelope (E) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.DepositorInsertE (E SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceMay 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR207 SARS-CoV-2 ORF3A_nostop
Plasmid#149319PurposeGateway-compatible Entry vector, with insert of ORF3A gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.DepositorInsertORF3A (ORF3a SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceMay 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR207 SARS-CoV-2 M_nostop
Plasmid#149322PurposeGateway-compatible Entry vector, with insert of the membrane (M) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.DepositorInsertM (M SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceMay 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 SARS-CoV-2 ORF10wu
Plasmid#149332PurposeGateway-compatible Entry vector, with insert of ORF10wu gene's CDS from SARS-CoV-2 genome definition in Wu et al, Nature 2020DepositorInsertORF10wu (ORF10 SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceMay 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 SARS-CoV2 TEV-NSP4
Plasmid#154403PurposeGateway-compatible Entry vectorDepositorInsertNSP4 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vector, with insert of n…TagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJuly 1, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR207 SARS-CoV-2 ORF7A_nostop
Plasmid#149324PurposeGateway-compatible Entry vector, with insert of ORF7A gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.DepositorInsertORF7A (ORF7a SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceMay 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 SARS-CoV-2 NSP5 Cys146Ala
Plasmid#154028PurposeGateway-compatible Entry vectorDepositorInsertNSP5
UseGateway-compatible entry vector, with mutagenized…TagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJune 25, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 SARS-CoV2 TEV-NSP16
Plasmid#154414PurposeGateway-compatible Entry vectorDepositorInsertNSP16 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vector, with insert of n…TagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJuly 2, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 SARS-CoV2 TEV-NSP14
Plasmid#154412PurposeGateway-compatible Entry vectorDepositorInsertNSP14 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vector, with insert of n…TagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJuly 2, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 SARS-CoV-2 ORF9B_nostop
Plasmid#149327PurposeGateway-compatible Entry vector, with insert of ORF9B gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.DepositorInsertORF9B
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceMay 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 SARS-CoV-2 ORF9Bwu
Plasmid#149331PurposeGateway-compatible Entry vector, with insert of ORF9Bwu gene's CDS from SARS-CoV-2 genome definition in Wu et al, Nature 2020DepositorInsertORF9Bwu
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceMay 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
BPV00605 pET21GG2-His(6)-BECN(1-265)
Plasmid#136686PurposeExpresses the Beclin-1 IDR+CC domains (a.a. 1-265) with a N-terminal hexa-Histidine tagDepositorInsertBeclin-1 IDR + CC (BECN1 Human)
UseTagsHexa-HistadineExpressionBacterialMutationDNA optimized for E. coli expressionPromoterT7Available sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only