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Showing: 9151 - 9200 of 14291 results

  3. S+Q111R+N122D

    Plasmid
    #167175
    Purpose
    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with Q111R and N122D mutations in Sf9 cells
    Depositor
    Susanne Dobler
    Article
    Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.
    Inserts
    ATP1A1-S+Q111R+N122D
    ATP1B1
    Use
    Tags
    Expression
    Bacterial and Insect
    Mutation
    Changed Q at 111 to R and changed N at 122 to D
    Promoter
    P10 and PH
    Available since
    Feb. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    167175-plasmid-map-sequence-id-334477

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  4. pME-mCherry-PA-Rac1

    Plasmid
    #169042
    Purpose
    Middle entry vector for Gateway cloning that contains an mCherry fused with an optimized photoactivatable Rac1 gene insert
    Depositor
    Paola Arlotta
    Article
    Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d
    Insert
    PA-Rac-1 (RAC1 Avena sativa (oat), Human)
    Use
    Gateway entry cloning vector
    Tags
    6X His and Fused with mCherry
    Expression
    Mutation
    Rac1 starts at I4 and contains mutations Q61L, E9…
    Promoter
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    169042-plasmid-map-sequence-id-341784

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  7. JDW 249 (pTol2-Dll4-F2-ETS#2-WT-8X-E1b-EGFP)

    Plasmid
    #156417
    Purpose
    8X copies of the ETS#2 site of the murine Dll4 intronic enhancer and a minimal E1b reporter driving expression of EGFP flanked by Tol2 sites
    Depositor
    Joshua Wythe
    Article
    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC
    Insert
    murine Dll4 F2-6/F8 ETS site B/ site #2, 8X
    Use
    Zebrafish transgenesis
    Tags
    Expression
    Mutation
    Promoter
    E1b min pro/b-globin intron
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    156417-plasmid-map-sequence-id-308709

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  18. pBIG2abc_hsHAUScomplex_FL

    Plasmid
    #202203
    Purpose
    Baculoviral transfer vector to co-express 8 subunits of human augmin complex
    Depositor
    Thomas Surrey
    Article
    Ustinova et al J Cell Sci. 2023 Jun 15;136(12):jcs26109
    Inserts
    HAUS1 (HAUS1 codon-optimized form for expression in Trichoplusia ni cells, Human)
    HAUS3 (HAUS3 codon-optimized form for expression in Trichoplusia ni cells, Human)
    HAUS2 (HAUS2 codon-optimized form for expression in Trichoplusia ni cells, Human)
    HAUS6 (HAUS6 codon-optimized form for expression in Trichoplusia ni cells, Human)
    HAUS7 (HAUS7 codon-optimized form for expression in Trichoplusia ni cells, Human)
    HAUS4 (HAUS4 codon-optimized form for expression in Trichoplusia ni cells, Human)
    HAUS5 (HAUS5 codon-optimized form for expression in Trichoplusia ni cells, Human)
    HAUS8 (HAUS8 codon-optimized form for expression in Trichoplusia ni cells, Human)
    Use
    Tags
    -mGFP-TEV-TwinStrep and His-tag (6x)
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin promoter
    Available since
    Sept. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    202203-plasmid-map-sequence-id-407808

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  23. hfCas12Max

    Plasmid
    #195336
    Purpose
    vector for encoding a human codon-optimized hfCas12Max driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.
    Insert
    human codon-optimized hfCas12Max
    Use
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    N243R, E336R, D892R
    Promoter
    CBh, CMV, hU6
    Available since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    195336-plasmid-map-sequence-id-389250

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  24. pAAV-CAG-DIO-PPO-Venus

    Plasmid
    #139504
    Purpose
    AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the CAG promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.
    Depositor
    Michael Bruchas
    Article
    Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11
    Has Service
    AAV5
    Insert
    parapinopsin
    Use
    AAV
    Tags
    Venus tag following 3x Ala linker
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    139504-plasmid-map-sequence-id-276180

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  25. pUCM-CLYBL-hNIL

    Plasmid
    #105841
    Purpose
    Donor construct for introduction of hNIL factors to CLYBL safe harbor site and iPSC differentiation to motor neuron
    Depositor
    Michael Ward
    Article
    Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    ISL LIM Homeobox 1 (ISL1 Human)
    LIM Homeobox 3 (LHX3 Human)
    mCherry
    rtTA3G
    Use
    CRISPR and TALEN
    Tags
    Expression
    Mutation
    Promoter
    CAG, EF-1alpha, and TRE3G
    Available since
    July 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    105841-plasmid-map-sequence-id-260007

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  27. pGL4.22-PGK1-HRE::dLUC

    Plasmid
    #128095
    Purpose
    Hypoxia response element real-time luciferase reporter
    Depositor
    Chi Van Dang
    Article
    Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:
    Insert
    Hypoxia response element (x3) from PGK1 promoter (PGK1 Human)
    Use
    Luciferase
    Tags
    drives expression of destabilized luciferase (2CP…
    Expression
    Mammalian
    Mutation
    Promoter
    inserted PGK1 hypoxia response elements (HRE) (x3)
    Available since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    128095-plasmid-map-sequence-id-249667

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  28. PX458-3xHA-en31FnCas9

    Plasmid
    #201949
    Purpose
    Mammalian expression plasmid of enhanced activity FnCas9 variant en31FnCas9 with T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Debojyoti Chakraborty
    Article
    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:
    Insert
    3xHA-NLS-en31FnCas9-T2A-EGFP
    Use
    CRISPR
    Tags
    3xHA, NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    G1243T, E1369R, E1603H on FnCas9
    Promoter
    Cbh
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    201949-plasmid-map-sequence-id-405303

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  30. ACBE-Q

    Plasmid
    #204608
    Purpose
    Mammalian expression of high-accuracy adenine-to-cytosine base editor ACBE-Q
    Depositor
    Dali Li
    Article
    Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103
    Insert
    bpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e (N108Q)-linker-C terminal of nSpCas9-bpNLS-P2A-GFP
    Use
    CRISPR
    Tags
    6*His
    Expression
    Mammalian
    Mutation
    TadA-8e (N108Q); mAAG (R165E/Y179F); S. pyogenes …
    Promoter
    Available since
    Aug. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    204608-plasmid-map-sequence-id-404717

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  31. dCas12Max-CBE

    Plasmid
    #195337
    Purpose
    vector for encoding a human codon-optimized dCas12Max-CBE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.
    Insert
    human codon-optimized dCas12Max-CBE
    Use
    Tags
    Expression
    Mammalian
    Mutation
    N243R, E336R, D1049A
    Promoter
    CBh, CMV, hU6
    Available since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    195337-plasmid-map-sequence-id-383131

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  32. dCas12Max-ABE

    Plasmid
    #195335
    Purpose
    vector for encoding a human codon-optimized dCas12Max-ABE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.
    Insert
    human codon-optimized dCas12Max-ABE
    Use
    Tags
    Expression
    Mammalian
    Mutation
    N243R, E336R, D1049A
    Promoter
    CBh, CMV, hU6
    Available since
    March 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    195335-plasmid-map-sequence-id-383124

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  33. MLRV2: NF-kb-SMAD-DBE-P53-AP1

    Plasmid
    #124536
    Purpose
    Multiplex luciferase reporter vector, with luciferase reporters for the pathways NFKB, SMAD, FOXO, P53 and AP1
    Depositor
    Koen Venken
    Article
    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1
    Inserts
    5 copies of the NF-kb DNA binding motif
    7xSMAD_RE::FLuc
    3xDBE_BE::Renilla (FOXO)
    2xP53_RE::NLuc
    6xAP-1_RE::GrRenilla
    Use
    Luciferase and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    124536-plasmid-map-sequence-id-272178

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  35. pWZL-Neo-Myr-Flag-DEST

    Plasmid
    #15300
    Purpose
    a Gateway-compatible retroviral destination vector which adds a myristoylation sequence and a FLAG tag to each introduced ORF
    Depositor
    Jean Zhao
    Article
    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    Flag and Myr
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 12, 2007
    Availability
    Academic Institutions and Nonprofits only
    15300-plasmid-map-sequence-id-190527

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  38. pLenti-TO/CMV-puro-DD-PAmCherry1-KRas G12D

    Plasmid
    #177320
    Purpose
    Lentiviral vector expressing PAmCherry1-tagged KRas 4B G12D mutant, with an N-terminal dimerization domain (DD).
    Depositor
    Xiaolin Nan, Steven Chu
    Article
    Nan et al Proc Natl Acad Sci U S A. 2015 Jun 30;11
    Insert
    KRAS 4B (KRAS Human)
    Use
    Lentiviral
    Tags
    FKBP-derived dimerization domain (DD, or DmrB) an…
    Expression
    Mammalian
    Mutation
    G12D
    Promoter
    TetOn-CMV
    Available since
    Jan. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    177320-plasmid-map-sequence-id-353281

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  40. PX458-3xHA-SpCas9-HF1

    Plasmid
    #201952
    Purpose
    Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Debojyoti Chakraborty
    Article
    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:
    Insert
    Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA
    Use
    CRISPR
    Tags
    3xHA, NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Cbh
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    201952-plasmid-map-sequence-id-405306

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Showing: 9151 - 9200 of 14291 results