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Plasmid#218025PurposeRESOLUTE SLC binder expression plasmidDepositorInsertBN002V-9
UseTags1xFLAG 10xHis and PelBExpressionBacterialMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-22b(+)_PelB-BN009K-H-FLAG-His
Plasmid#218049PurposeRESOLUTE SLC binder expression plasmidDepositorInsertBN009K-H
UseTags1xFLAG 10xHis and PelBExpressionBacterialMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-22b(+)_PelB-BN00AZ-W-FLAG-His
Plasmid#218039PurposeRESOLUTE SLC binder expression plasmidDepositorInsertBN00AZ-W
UseTags1xFLAG 10xHis and PelBExpressionBacterialMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-22b(+)_PelB-BN00H3-S-FLAG-His
Plasmid#218038PurposeRESOLUTE SLC binder expression plasmidDepositorInsertBN00H3-S
UseTags1xFLAG 10xHis and PelBExpressionBacterialMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-22b(+)_PelB-BN00FH-2-FLAG-His
Plasmid#218037PurposeRESOLUTE SLC binder expression plasmidDepositorInsertBN00FH-2
UseTags1xFLAG 10xHis and PelBExpressionBacterialMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-22b(+)_PelB-BN00B0-E-FLAG-His
Plasmid#218035PurposeRESOLUTE SLC binder expression plasmidDepositorInsertBN00B0-E
UseTags1xFLAG 10xHis and PelBExpressionBacterialMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-29b(+)_notufA-CmEndoCOM_3C-CBD-His
Plasmid#218198PurposeBacterial expression of a His-tagged Chitin Binding fusion of Cordyceps militaris EndoCOMDepositorInsertEndoCOM
UseTags3C-CBD-HisExpressionBacterialMutationcodon optimized Ecoli, residues 19-315PromoterT7Available sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-29b(+)_notufA-EmEndoF2-CBD-His
Plasmid#218197PurposeBacterial expression of a His-tagged Chitin Binding fusion of Elizabethkingia miricola EndoF2DepositorInsertEndoF2
UseTagsCBD-HisExpressionBacterialMutationcodon optimized Ecoli, residues 45-335PromoterT7Available sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-His6-SUMO-cSrc-SH2-AviTag
Plasmid#214230PurposeBacterial expression of SH2 domain of the cSrc kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selectionsDepositorInsertcSrc kinase SH2 domain (SRC Human)
UseTags6xHis, AviTag, and SUMOExpressionBacterialMutationPromoterT7Available sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
Anti-Keyhole limpet hemocyanin [N132A/12R]
Plasmid#206532PurposeMammalian Expression Plasmid of anti-Keyhole limpet hemocyanin (Giant keyhole limpet). Derived from hybridoma N132A/12.DepositorInsertanti-Keyhole limpet hemocyanin (Megathura crenulata) recombinant Mouse monoclonal antibody
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt7 (136-403) L361C
Plasmid#213622PurposeBacterial expression of SUMO-tagged mouse syt7 (136-403) L361CDepositorInsertsyt7 (136-403) L361C (Syt7 Mouse)
UseTagsSUMOExpressionBacterialMutationC260A C275A L361CPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET 15b+His SUMO Rab12 (Q101L)
Plasmid#208371PurposeBacterial expression of His-SUMO-tagged human Rab12 (Q101L)DepositorInsertHis SUMO Rab12 (Q101L) (RAB12 Human)
UseTagsExpressionMammalianMutationQ101LPromoterAvailable sinceDec. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-Duet-6xHis-TEV-Nsp8(76-198)
Plasmid#201024PurposeBacterial expression of codon optimized SARS-CoV-2 nsp8 residues 76-198, with an N-terminus His tag followed by a TEV cleavage site.DepositorInsertnon-structural protein 8 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
UseTags6xHis-TEVExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETDUET-GST-AUP1 379-410:Ube2G2
Plasmid#185335PurposeUsed for co-expression of the AUP1 G2BR with UBE2G2 for crystallographyDepositorInsertAUP1 aa 379-410, Ube2G2 (AUP1 Mouse, Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NcQDE-2_506-938-del787-838GSG_K
Plasmid#146730PurposeBacterial Expression of NcQde-2_506-938-del787-838GSGDepositorInsertNcQde-2_506-938-del787-838GSG
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28-6xhis-sumo-CeMIGA(106-496)
Plasmid#192832PurposeExpress C.elegans MIGA fragment 106-496 in bacteriaDepositorInsertMitoguardin homologue (miga-1 Nematode)
UseTags6xhis sumoExpressionBacterialMutationPromoterT7Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
human KANK2-A670V Ankyrin Repeats pET151
Plasmid#164055PurposeExpresses human KANK2 Ank domain - A670V mutant in bacterial cellsDepositorInsertKidney Ankyrin Repeat-Containing Protein 2 A670V mutant (KANK2 Human)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
mouse KANK4-Y801H Ankyrin Repeats pET151
Plasmid#164058PurposeExpresses mouse KANK4 Ank domain -Y801H mutant in bacterial cellsDepositorInsertKidney Ankyrin Repeat-Containing Protein 4 Y801H mutant (Kank4 Mouse)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1-LanCL2-C321A/C367A-C-Ter-His
Plasmid#154188PurposeTo express LanCL2-C321A/C367A in bacterial cellsDepositorInsertLanCL2
UseTagsHexahistidineExpressionBacterialMutationCys321->Ala321; Cys367->Ala367PromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1024-Tier1-PRPBSA-YPet-p2A-PuroR
Plasmid#169499PurposeTier-1 vector encoding PRPBSA-driven YPet in combination with a marker for puromycine selection (PRPBSA-YPet-p2A-PuroR-pA)DepositorInsertPRPBSA-driven YPet-P2aA-PuroR
UseTagsExpressionMammalianMutationPromoterPRBSAAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only