pETDUET-GST-AUP1 379-410:Ube2G2
(Plasmid #185335)

• Purpose: Used for co-expression of the AUP1 G2BR with UBE2G2 for crystallography
• Depositing Lab: Allan Weissman
• Publication: Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. doi: 10.1371/journal.pbio.3001474. eCollection 2021 Dec.

Backbone

• Vector backbone: pETDUET
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AUP1 aa 379-410, Ube2G2
• Species: H. sapiens (human), M. musculus (mouse)
• Entrez Gene: AUP1
• Tag / Fusion Protein:
  • GST (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI, NdeI (unknown if destroyed)
• 3′ cloning site: HindII, XhoI (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Encodes G2BR of AUP1 and UBE2G2 (from mouse cDNA - amino acid sequence identical to human).