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pYTK083
Plasmid#65190PurposeEncodes AmpR-ColE1 as a Type 8 part to be used in the Dueber YTK systemDepositorInsertAmpR-ColE1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
4xmts-mScarlet-I
Plasmid#98818PurposeIn vivo visualization of mitochondria (can be used for colocalization studies)DepositorArticleInsertmts
UseTagsmScarlet-IExpressionMammalianMutationPromoterCMVAvailable sinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_2FLAG-AKAP79ΔPKA
Plasmid#183452PurposeExpression of human AKAP79 lacking the PKA binding site (Δ391-400)DepositorInsertAKAP5 (AKAP5 Human)
UseTags2xFLAGExpressionMammalianMutationaa391-400 deletedPromoterCMVAvailable sinceJune 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-SRPK1
Plasmid#23582DepositorInsertSRPK1 (SRPK1 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pKmK.I3R
Plasmid#125065PurposeStorage plasmid for K.marxianus right homology armDepositorInsertRight homology arm for targeting integration to SWF1/ARO1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKmK.I1R
Plasmid#125063PurposeStorage plasmid for K.marxianus right homology armDepositorInsertRight homology arm for targeting integration to LAC4
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
LeGO-S2
Plasmid#85211PurposeLentiviral vector expressing T-Sapphire fluorescent protein driven by SFFV promoterDepositorInsertT-Sapphire
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceJan. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKmK.I1L
Plasmid#125030PurposeStorage plasmid for K.marxianus left homology arm; type 1 partDepositorInsertLeft homology arm for targeting integration to LAC4
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKmK.I3L
Plasmid#125032PurposeStorage plasmid for K.marxianus left homology arm; type 1 partDepositorInsertLeft homology arm for targeting integration to SWF1/ARO1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBRPB CAG-mCherry-IP
Plasmid#106333PurposePiggyBac transposon, mCherry-IRES-puro labeling under the control of CAG promoter. Used in chimera experiment of ES cells.DepositorInsertmCherry-IRES-Puro
UsePiggybac transposonTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
lenti sgRNA(MS2)_puro optimized backbone
Plasmid#73797Purposeoptimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 and EF1AAvailable sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
TFORF0548
Plasmid#143041PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPAX8 (PAX8 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/Flag-METTL14
Plasmid#53740PurposeFor expression of METTL14 (methyltransferase-like 14) in human cellsDepositorInsertMethyltransferase-like 14 (METTL14 Human)
UseTagsFLAG tagExpressionMammalianMutationPromoterT7Available sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pU6-(BbsI)_CBh-Cas9-T2A-mCherry
Plasmid#64324PurposeExpression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to mCherry via a T2A peptideDepositorInsertsCas9
sgRNA cassette
UseCRISPRTags3xFLAG, NLS, and T2A-mCherryExpressionMammalianMutationPromoterCBh and U6Available sinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAO122 (Apr LEU2 MRC1)
Plasmid#41923DepositorInsertMediator of the Replication Checkpoint
UseTagsExpressionMutationPromoterAvailable sinceAug. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLAIΔmls
Plasmid#24594DepositorInsertHIV-1
UseTagsExpressionMutationHuman immunodeficiency virus type 1, isolate BRU,…PromoterAvailable sinceJune 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
IA001: pMVP (L3-L2) 3x HA epitope tag+ polyA
Plasmid#121750PurposepMVP L3-L2 entry plasmid, contains 3x HA epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.DepositorInsert3x HA epitope tag + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF3529
Plasmid#145005PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertVDR (VDR Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST-HisMBP-SED1
Plasmid#178191PurposeSED1 biosensor for expression in Escherichia coliDepositorInsertSED1 biosensor
UseTags6xHis-MBPExpressionBacterialMutationPromotertacAvailable sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP-GFP-LC3-RFP
Plasmid#84573PurposeExpresses GFP-LC3-RFP in mammalian cells to measure autophagic fluxDepositorInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
UseRetroviralTagsEGFP and mRFP1ExpressionMammalianMutationPromoterAvailable sinceJan. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pL0M-S-mKOκ-EC18154
Plasmid#137077PurposeGolden Gate Level 0 S module for N-terminal fluorescent protein taggingDepositorInsertmKOκ DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Rab4a-7
Plasmid#56440PurposeLocalization: Ras GTPase, Excitation: 488, Emission: 507DepositorInsertRab4a (RAB4A Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 29, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-scFv-T
Plasmid#67843PurposepET-30-based vector dedicated to efficient scFv expression, which circumvents the problem of in-frame amber codons in scFvs.DepositorInsertantibody 1D8 light kappa chain
UseTagsHis-tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAO139 (Apr LEU2 mrcl-AQ)
Plasmid#41924DepositorInsertMediator of the Replication Checkpoint
UseTagsExpressionMutationMRC1AQPromoterAvailable sinceAug. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO V5 DNAJB7
Plasmid#19530DepositorInsertDnaJ (Hsp40) homolog, subfamily B, member 7 (DNAJB7 Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pYTK077
Plasmid#65184PurposeEncodes KanamycinR as a Type 6 part to be used in the Dueber YTK systemDepositorInsertKanamycinR
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO DNAJB3
Plasmid#19471DepositorInsertDNAJB3 (Dnajb3 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRha-tns
Plasmid#170630PurposeExpression of CRISPR-associated transposasesDepositorInsertVchTnsABC
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
MAP2K4 gRNA (BRDN0001145028)
Plasmid#76650Purpose3rd generation lentiviral gRNA plasmid targeting human MAP2K4DepositorInsertMAP2K4 (MAP2K4 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only
