-
Plasmid#143660PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertDACH2 (DACH2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FGFR4
Plasmid#116743PurposeLentiviral expression of FGFR4DepositorInsertFGFR4 (FGFR4 Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDH_Str-KDEL_ManII-SBP-EGFP
Plasmid#65258Purposesynchronize trafficking of ManII from the ER (RUSH system)DepositorInsertStreptavidin-KDEL and truncated Mannosidase II
UseLentiviralTagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMutationcontains only amino acids 1 to 116 of ManIIPromoterCMVAvailable sinceAug. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
dCas9-dMSK1
Plasmid#165602PurposeExpresses Sp dCas9 fused to truncated human MSK1 (42-802)DepositorInsertS. Pyogenes dCas9 with c-terminal truncated human Mitogen- and stress-activated protein kinase-1 (42-802) (RPS6KA5 Synthetic, S. Pyogenes, Human)
UseCRISPR and LentiviralTagsFLAG TagExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
TFORF2195
Plasmid#142181PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMLXIPL (MLXIPL Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0661
Plasmid#143062PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertCHD7 (CHD7 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
HiFi Cas9
Plasmid#188490PurposeExpresses FLAG tagged HiFi Cas9DepositorInsertCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationR691APromoterEF1aAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
FSW_SUN1_GFP
Plasmid#205985PurposeExpresses hSyn1-driven, GFP-tagged SUN1 to label neuronal nucleiDepositorInsertSUN1 (Sun1 Mouse)
UseLentiviralTagsSuperfolder GFPExpressionMutationPromoterhSyn1Available sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFUW-TetO-DsRed-cNLS
Plasmid#138533PurposeLentiviral inducible expression of DsRed-Express2 with nuclear localization signalDepositorInsertDsRed-Express2
UseLentiviralTags3x SV40 cNLSExpressionMammalianMutationPromoterTRE-CMVAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
DRH334: FCK-QuasAr2-mO2
Plasmid#51692PurposeFluorescent reporter of membrane voltage with improved sensitivityDepositorInsertQuasAr2-mOrange
UseLentiviralTagsmOrange2ExpressionMammalianMutationPromoterCaMKIIaAvailable sinceApril 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-FOXM1c
Plasmid#68810PurposeDoxycycline inducible lentiviral vector for FOXM1c expression in mammalian cellsDepositorInsertFOXM1c (FOXM1 Human)
UseLentiviral; Doxycycline inducibleTagsFLAGExpressionMammalianMutationPromoterTRE promoter, Tet ONAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1 tTA SDHB
Plasmid#177977PurposeAll-in-one dox-repressible expression of cDNADepositorInsertSDHB (SDHB Human)
UseLentiviralTagsExpressionMammalianMutationSilent point mutations to SDHB sgRNA targetPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRDA_078
Plasmid#158582PurposeLentiviral expression of BE4max and customizable gRNADepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationCas9 D10APromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLU-CMV-PL3 Flag-humanTRAF6-WT (1111)
Plasmid#66923PurposeLentiviral Expression of TRAF6DepositorInsertTRAF6 (TRAF6 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pscALPS- HIV2-ROD vpx
Plasmid#115816PurposeEncodes codon optimized 3xFLAG-HIV2ROD Vpx (M15390) and puromycin resistance proteinDepositorInsertHIV2ROD Vpx (M15390)
UseLentiviralTags3x-FLAGExpressionMutationPromoterSFFVAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-TRE3G-dCas9-EGFP-HaloTag
Plasmid#169451Purposeexpression of dCas9-EGFP-HaloTagDepositorInsertdCas9-EGFP-HaloTag
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
pSLQ11274:pHR-hU6-crTet-HSP-hyperdCas12a-miniVPR-CMV-mCherry
Plasmid#210603Purposeheat shock promoter - activated hyperdCas12a-based transcriptional activatorDepositorInserthyperdCas12a-miniVPR
UseCRISPR and LentiviralTagsExpressionMutationPromotertruncated hsp70Available sinceDec. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLEX-FHH-MTORKinaseDomain
Plasmid#120708PurposeExpresses Flag-HA-6xHIS-Mtor Kinase Domain fusion protein in mammalian cells & for virus productionDepositorInsertMTOR Kinase Domain (MTOR Human)
UseLentiviralTagsFlag-HA-6xHISExpressionMammalianMutationAmino acids 1967-2549PromoterCMVAvailable sinceJan. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
psd44-GqQL
Plasmid#46826Purpose2nd generation lentiviral vector for expression of mutant G protein alpha q in mammalian cellsDepositorInsertg protein alpha q (GNAQ Human)
UseLentiviralTagsExpressionMammalianMutationQ209L (The mutation reduces GTPase activity resul…PromoterPGK promoterAvailable sinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only