FSW_SUN1_GFP
(Plasmid #205985)

• Purpose: Expresses hSyn1-driven, GFP-tagged SUN1 to label neuronal nuclei
• Depositing Lab: Ronald Hart
• Publication: Hart Lab Plasmids (unpublished)

Backbone

• Vector backbone: FUGW
• Backbone size w/o insert (bp): 7961
• Total vector size (bp): 10833
• Modifications to backbone: Replaced UbiC promoter with hSyn1 promoter
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: SUN1
• Species: M. musculus (mouse)
• Insert Size (bp): 1650
• Entrez Gene: Sun1 (a.k.a. 4632417G13Rik, 5730434D03Rik, Unc84a, mKIAA0810)
• Promoter: hSyn1
• Tag / Fusion Protein:
  • Superfolder GFP (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: gggaggggaaacaggatg

Resource Information

• Supplemental Documents:
  • FSW_SUN1_GFP.gbk
• A portion of this plasmid was derived from a plasmid made by: Insert was recloned from AddGene # 160141

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Lentiviral vector to express fused Sun1-GFP in neurons for enriching nuclear preparations.