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Plasmid#106438PurposeEncodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome.DepositorInsertNOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Hyg
Plasmid#60524PurposeSB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: hygromycin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
GW1CMV-Perceval
Plasmid#21737Purposecan be used to monitor the ATP:ADP ratio during live-cell imagingDepositorInsertPerceval (fluorescent ATP:ADP sensor)
UseTags5'UTRExpressionMammalianMutationimproved Kozak sequencePromoterAvailable sinceAug. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLPCX cyto Grx1-roGFP2
Plasmid#64975PurposeMammalian expression of cytosolic Grx1-roGFP2 (retroviral vector)DepositorInsertGlutaredoxin-1 (GLRX Human)
UseRetroviralTagsroGFP2ExpressionMammalianMutationPromoterAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+ hnRNPK myc HIS
Plasmid#71662PurposeMammalian expression of hnRNPKDepositorInserthnRNPK (HNRNPK Human)
UseTagsHIS and mycExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_SBP-mCherry-Ecadherin
Plasmid#65287Purposesynchronize trafficking of Ecadherin from the ER (RUSH system)DepositorInsertEcadherin
UseTagsIL-2 Signal Sequence and Streptavidin Binding Pro…ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pScarlessHD-sfGFP-DsRed
Plasmid#80811PurposeVector containing a cassette for scarless insertion of sfGFP and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.DepositorInsertsfGFP-piggyBac-DsRed
UseUnspecifiedTagssfGFPExpressionMutationPromoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 PRDM16
Plasmid#15503DepositorInsertPRDM16 (Prdm16 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 14, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCDH-PGK
Plasmid#72268PurposeExpress gene of interest under PGK (phosphoglycerate kinase I) promoterDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceJan. 11, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBABEbleo-Flag-BRAFV600E
Plasmid#53156PurposeExpresses Flag tagged human oncogenic BRAFV600E from bleomycin resistance retroviral vectorDepositorInsertBRAF (BRAF Human)
UseRetroviralTagsFLAGExpressionMammalianMutationV600EPromoterAvailable sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRK5-TGF beta Type II receptor Flag
Plasmid#31719DepositorInsertTGF beta Type II Receptor (TGFBR2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
CMV-ER-LAR-GECO1
Plasmid#61244PurposeExpresses LAR-GECO1 in the endoplasmic reticulum in mammalian cellsDepositorInsertLAR-GECO1
UseTagsER-retention sequence: KDEL and ER-targeting sequ…ExpressionMammalianMutationSubstitutions relative to R-GECO1: V51W/I113V/N35…PromoterCMVAvailable sinceMarch 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
MCS-BioID2-HA pBabe-puro
Plasmid#120308PurposeTo fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present upstream of BioID2 tag and HA tagDepositorTypeEmpty backboneUseRetroviralTags-BioID2-HAExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pLNCX2-mCherry-VPS4A
Plasmid#115334Purposeexpresses mCherry-VPS4A in mammalian cellsDepositorInsertVPS4A (VPS4A Human)
UseRetroviralTagsmCherryExpressionMammalianMutationsilent mutation in position 4050 C to TPromoterCMVAvailable sinceSept. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCytERM_mScarlet_N1
Plasmid#85066PurposeIn vivo visualization of the ER (can be used for colocalization studies and the OSER assay)DepositorInsertCytERM
UseTagsmScarletExpressionMammalianMutationaa1-29PromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV/TO Puro empty (w175-1)
Plasmid#17482Purpose3rd gen lentiviral empty control vector, CMV/TO promoter, PuroDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCBC-DT2T3
Plasmid#50591PurposeCRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sitesDepositorInsertT2, gRNA scaffold, U6-29t plus, U6-1p, T3
UseCRISPR; Pcr templateTagsExpressionPlantMutationPromoterAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTRK892
Plasmid#71803PurposeExpression of mutated L. gasseri gusA from strong Ppgm promoterDepositorInsertgusA3
UseTagsExpressionBacterialMutationD573APromoterPpgmAvailable sinceJan. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV-Azurite
Plasmid#36086Purpose3rd generation lentiviral plasmidDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 1, 2012AvailabilityAcademic Institutions and Nonprofits only