Showing: 3621 - 3640 of 3839 results
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Plasmid#138150PurposeFor cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaaDepositorInserthU6_Rfxcas13d-DR1_BsmBI
UseLentiviralTagsExpressionMutationDR1 A>TPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-EGFP (BPK1098)
Plasmid#133962PurposeCMV and T7 promoter plasmid for EGFP expression in mammalian cells or for in vitro transcriptionDepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterCMV and T7AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_EGFP_NbALFA
Plasmid#136626PurposePlasmid encoding the bacterial expression of NbALFA for ALFA-tag as an EGFP-fusionDepositorInsertEGFP-NbALFA
UseTagsHisx10 and StrepExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
Cbh_v5 AAV-ABE N-terminal
Plasmid#137177PurposeAAV genome: expresses the N-terminal of v5 AAV-ABE from the Cbh promoterDepositorInsertv5 AAV-ABE N-terminal
UseAAVTagsExpressionMutationCas9 D10APromoterCbhAvailabilityAcademic Institutions and Nonprofits only -
Cbh_v5 AAV-ABE C-terminal
Plasmid#137178PurposeAAV genome: expresses the C-terminal of v5 AAV-ABE from the Cbh promoter, and U6-sgRNADepositorInsertv5 AAV-CBE C-terminal; U6-protospacer
UseAAVTagsExpressionMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pLS-SceI
Plasmid#137725PurposeLentivirus reporter assay plasmid that contains a I-SceI site and a EGFP reporter gene.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
LentiGuide Puro-P2A-EGFP
Plasmid#137729PurposeFor simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).DepositorTypeEmpty backboneUseCRISPR, Lentiviral, and Synthetic BiologyTagsEGFPExpressionBacterial and MammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
ER-mScarletI
Plasmid#137805PurposeVisualization of the Endoplasmic ReticulumDepositorArticleInsertKDEL
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SARS-CoV-2 S
Plasmid#149329PurposeGateway-compatible Entry vector, with insert of the spike (S) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorInsertS (S SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
pAV0286
Plasmid#149333PurposeBacterial expression of SENP_EuB proteaseDepositorInsertSENP_EuB protease
UseTagsHis14 and Tev protease cleavage siteExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pYL192 (TRV1)
Plasmid#148968PurposeTobacco Rattle Virus RNA 1; used for virus induced gene silencing in plants along with pYL156 (TRV RNA2)DepositorInsertTRV RNA1
UsePlant vector with trv rna1; this vector used with…TagsExpressionMutationP697S mutation in putative replicase. S239G and S…PromoterAvailabilityAcademic Institutions and Nonprofits only -
N8his-GFPenhancer-GGGGS4-LaG16
Plasmid#140442PurposeWe designed a flexible linker to connect two types of anti GFP nanobodies and used this fusion nanobody to purify GFP tagged protein.DepositorInsertGFPenhancer-GGGGS4-LaG16
UseTags8 histidine tagExpressionBacterialMutationPromoterT7 promotorAvailabilityAcademic Institutions and Nonprofits only -
pMK381 (AAVS1 CMV-OsTIR1F74G)
Plasmid#140536PurposeAAVS1 CMV-OsTIR1(F74G)DepositorInsertCMV-OsTIR1(F74G)
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-GRAB_5-HT1.0
Plasmid#140552PurposeExpresses the 5-HT sensor GRAB_5-HT1.0 in neuronsDepositorHas ServiceAAV9Insert5-HT sensor GRAB_5-HT1.0
UseAAVTagsExpressionMutationPromoterhSynAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-SARS-CoV-2-S-RBD-Fc
Plasmid#141183PurposeMammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with IgG1-FcDepositorInsertSecreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 fused to Fc of IgG1 (S SARS coronavirus 2)
UseTagsFc region of IgG1 (a.a. Asp-221 to Lys-447) and S…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-SARS-CoV-2-S-RBD-sfGFP
Plasmid#141184PurposeMammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with sfGFPDepositorHas ServiceCloning Grade DNAInsertS surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ] (S SARS coronavirus 2)
UseTagsSignal peptide from influenza HA and Superfolder …ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-ACE2
Plasmid#141185PurposeMammalian expression plasmid for human ACE2 with an N-terminal (extracellular) c-myc tagDepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pDONR207 SARS-CoV-2 NSP3
Plasmid#141257PurposeGateway-compatible Entry vector, with insert of NSP3 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorUseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 SARS-CoV-2 NSP6
Plasmid#141260PurposeGateway-compatible Entry vector, with insert of NSP6 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorInsertNSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 SARS-CoV-2 NSP16
Plasmid#141269PurposeGateway-compatible Entry vector, with insert of NSP16 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorInsertNSP16 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailabilityIndustry, Academic Institutions, and Nonprofits
Showing: 3621 - 3640 of 3839 results