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Showing: 3601 - 3620 of 4003 results

  3. pUBC_mCherry_serinemod_E488QADAR_p2A_yGFP

    Plasmid
    #154786
    Purpose
    Expresses mCherry fused to hyperactive ADAR catalytic domain, control plasmid for HyperTRIBE
    Depositor
    Robert Singer
    Article
    Biswas et al iScience. 2020 Jun 28;23(7):101318. doi:
    Insert
    Adenosine deaminse (Adar Synthetic, Fly)
    Use
    Lentiviral
    Tags
    GFP, V5, and mCherry
    Expression
    Mammalian
    Mutation
    Synonymized serine to prevent autoediting of ADAR…
    Promoter
    UBC
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    154786-plasmid-map-sequence-id-306310

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  5. pUBC_stdMCP_serinemod_E488QADAR_p2A_yGFP

    Plasmid
    #154787
    Purpose
    Expresses synonymized MS2 coat protein dimer (MCP) fused to hyperactive ADAR catalytic domain
    Depositor
    Robert Singer
    Article
    Biswas et al iScience. 2020 Jun 28;23(7):101318. doi:
    Insert
    Adenosine deaminse (Adar Synthetic, Fly)
    Use
    Lentiviral
    Tags
    GFP, V5, and stdMCP
    Expression
    Mammalian
    Mutation
    Synonymized serine to prevent autoediting of ADAR…
    Promoter
    UBC
    Available since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    154787-plasmid-map-sequence-id-306329

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  8. pAWG-dCas9-VPR

    Plasmid
    #63802
    Purpose
    SP-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; drosophila vector
    Depositor
    George Church
    Article
    Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm
    Insert
    SP-dCas9-VPR
    Use
    CRISPR and Synthetic Biology
    Tags
    VPR (VP64-p65-Rta)
    Expression
    Insect
    Mutation
    Promoter
    act5c
    Available since
    May 14, 2015
    Availability
    Academic Institutions and Nonprofits only

  14. Gal4-entry+NLS

    Plasmid
    #128013
    Purpose
    Vector containing the Gal4-entry expression cassette with SV40 NLS and a separate expression cassette driving mCherry via the traffic jam enhancer
    Depositor
    Julius Brennecke
    Article
    Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-
    Insert
    Gal4 DBD-SV40NLS-3x-Flag
    Use
    Gal4 entry vector with nls to generate tethering …
    Tags
    Gal4-DBD-SV40NLS-3xFlag
    Expression
    Mutation
    Promoter
    Available since
    Aug. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    128013-plasmid-map-sequence-id-251575

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  16. SGyA

    Plasmid
    #160292
    Purpose
    Drives expression of Cas9 and a TdTomato fluorescent marker
    Depositor
    Omar Akbari
    Article
    Gamez et al Nat Commun. 2021 Dec 10;12(1):7202. doi:
    Inserts
    Vas promoter
    hSpCas9
    tdTomato
    CTCF insulator
    Gypsy insulator
    Left homology arm Y chromosome
    Right homology arm Y chromosome
    Use
    Tags
    T2A GFP
    Expression
    Insect
    Mutation
    Promoter
    Available since
    March 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    160292-plasmid-map-sequence-id-316542

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  17. pTIGER_tdTomato::3xFLAG::dCrk

    Plasmid
    #131138
    Purpose
    UASp construct for over-expression of Drosophila Crk with N-terminal tandem Tomato and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.
    Depositor
    Mark Peifer
    Article
    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.
    Insert
    Crk (Crk Fly)
    Use
    Tags
    3XFLAG and tdTomato
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Oct. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    131138-plasmid-map-sequence-id-258855

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  19. pTIGER_mNeonGreen::3xFLAG::dCrk

    Plasmid
    #131137
    Purpose
    UASp construct for over-expression of Drosophila Crk with N-terminal monomeric NeonGreen and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.
    Depositor
    Mark Peifer
    Article
    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.
    Insert
    Crk (Crk Fly)
    Use
    Tags
    3XFLAG and mNeonGreen
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Oct. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    131137-plasmid-map-sequence-id-258861

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  20. Pyr pUAST-HA

    Plasmid
    #69768
    Purpose
    Pyramus (FGF8-like-2) Ligand in P element-based pUAST vector for Gal4-regulated expression in Drosophila.
    Depositor
    Arno Muller
    Article
    Klingseisen et al Development. 2009 Jul;136(14):2393-402.
    Insert
    Pyramus (FGF8like-2) (pyr Fly)
    Use
    P element-based puast vector for gal4-regulated e…
    Tags
    HA-Tag
    Expression
    Insect
    Mutation
    241T amino acid insertion compared to reference s…
    Promoter
    hsp70 promoter
    Available since
    Nov. 24, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 3601 - 3620 of 4003 results