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Showing: 3581 - 3600 of 8972 results

  1. pCAS9i_TRP

    Plasmid
    #141253
    Purpose
    Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Adam Bertl
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 (Cas9), pSNR52 (gRNA)
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141253-plasmid-map-sequence-id-308890

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  2. Peripherin/pAS2-1

    Plasmid
    #98135
    Purpose
    Peripherin (NM_006262) in DNA-binding domain (BD) vector, used for yeast-two hybrid
    Depositor
    Rita Shiang
    Insert
    Peripherin (PRPH Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 21, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    98135-plasmid-map-sequence-id-191140

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  3. pTH341-SUP45(139-437)-GalBD

    Plasmid
    #29741
    Depositor
    Tobias von der Haar
    Insert
    SUP45 (SUP45 Budding Yeast)
    Use
    Tags
    Gal Activation domain and Myc
    Expression
    Yeast
    Mutation
    Codons 139-437 of the yeast SUP45 gene only
    Promoter
    Available since
    Sept. 21, 2011
    Availability
    Academic Institutions and Nonprofits only
    29741-plasmid-map-sequence-id-13825

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  4. pTH339-SUP45(1-138)-GalBD

    Plasmid
    #29739
    Depositor
    Tobias von der Haar
    Insert
    SUP45 (SUP45 Budding Yeast)
    Use
    Tags
    Gal Activation domain and Myc
    Expression
    Yeast
    Mutation
    Codons 1-138 of the yeast SUP45 gene only
    Promoter
    Available since
    Sept. 13, 2011
    Availability
    Academic Institutions and Nonprofits only
    29739-plasmid-map-sequence-id-13823

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  5. pTH342-SUP45(276-437)-GalBD

    Plasmid
    #29742
    Depositor
    Tobias von der Haar
    Insert
    SUP45 (SUP45 Budding Yeast)
    Use
    Tags
    Gal Activation domain and Myc
    Expression
    Yeast
    Mutation
    Codons 276-437 of the yeast SUP45 gene only
    Promoter
    Available since
    Sept. 13, 2011
    Availability
    Academic Institutions and Nonprofits only
    29742-plasmid-map-sequence-id-13826

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  7. pRL_CT_GI_del1

    Plasmid
    #98744
    Purpose
    For red/far-red light-regulated gene expression in Saccharomyces cerevisiae. Integrates into the yeast ura3-52 locus. Encodes constitutively expressed PhyBNT-synTALE-DBD and PIF3-NLS-VP64AD fusions.
    Depositor
    Bernd Müller-Röber
    Article
    Hochrein et al Nucleic Acids Res. 2017 Sep 6;45(15):919
    Inserts
    phytochrome interacting factor 3 (PIF3 Mustard Weed)
    phytochrome B (PHYB Mustard Weed)
    Use
    Synthetic Biology
    Tags
    SV40-NLS, VP64-AD, and synTALE-DBD
    Expression
    Yeast
    Mutation
    mutated nucleotide 576 C to A, deleted amino acid…
    Promoter
    FBA1 and TDH3
    Available since
    Aug. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98744-plasmid-map-sequence-id-189858

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  16. YIP sHRPa-Aga1P

    Plasmid
    #73151
    Purpose
    Large fragment of split HRP fused to the N-terminus of the yeast mating protein Aga1P. When linearized, this vector incorporates into the yeast genome.
    Depositor
    Alice Ting
    Article
    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103
    Insert
    sHRPa-Aga1P
    Use
    Tags
    V5 tag
    Expression
    Yeast
    Mutation
    Promoter
    glyceraldehyde-3-phosphate dehydrogenase GPD prom…
    Available since
    June 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73151-plasmid-map-sequence-id-134538

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  19. pMM0638

    Plasmid
    #129005
    Purpose
    Integration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2
    Depositor
    Megan McClean
    Article
    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1
    Insert
    5' URA3 -pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-URA3 URA3 3'- KanR-ColE1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    129005-plasmid-map-sequence-id-261246

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  20. ZJOM25

    Plasmid
    #133672
    Purpose
    Integration of MSP1-2GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Present on mitochondria and peroxisomes.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-MSP1-2GFP (MSP1 Budding Yeast)
    Use
    Tags
    EGFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    133672-plasmid-map-sequence-id-267535

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Showing: 3581 - 3600 of 8972 results