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Plasmid#178529PurposeExpresses Stargazin-mEGFP-iLID in mammalian cellsDepositorInsertStargazin-mEGFP-iLID
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 beta-catenin Y654F
Plasmid#16072DepositorInsertbeta-catenin (CTNNB1 Chicken)
UseTagsEGFPExpressionMammalianMutationY654F, *(see below)PromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
AAV-SYN_mEGFP-CREB (S133A)
Plasmid#137010PurposeAAV backbone, mEGFP-CREB donor for G-CREB with S133A mutationDepositorInsertCREB1 (Creb1 Rat)
UseAAVTagsmEGFPExpressionMammalianMutationChanges Serine 133 into AlaninePromoterhSynapsinAvailable sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGTag-NLS-eGFP-B-actin
Plasmid#117812PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInserteGFP
UseSynthetic BiologyTagsNLSExpressionMutationPromoterAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUb-Cas9-EGFP_cU6:6-sgRNA
Plasmid#190597PurposeThe plasmid encodes S. pyogenes Cas9 and eGFP separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter.DepositorInsertsCas9
sgRNA
UseCRISPRTagsEGFP - separated by T2AExpressionMutationPromoterAedes aegypti polyubiquitin and Culex quinquefascā¦Available sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-FOXF1-MUT
Plasmid#194569PurposeMammalian Expression of mEGFP-FOXF1 Mutant (A231Rfs*61) Fusion ProteinDepositorInsertFOXF1 (FOXF1 Human)
UseTagsmEGFPExpressionMammalianMutationA231Rfs*61PromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-NLS-Vx3-mEGFP
Plasmid#35529DepositorInsertNLS-Vx3
UseRetroviralTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_Dual_GST-TEV-EGFP-TBK1
Plasmid#187830PurposePlasmid for the expression and purification of GST-TEV-EGFP-TBK1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1631DepositorInsertTBK1 (TBK1 Human)
UseTagsGST and TEV-mEGFP-GG LinkerExpressionBacterial and InsectMutationPromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-FLAG-GFP-Ubl4A
Plasmid#86921PurposeTo express Ubl4A tagged with FLAG-EGFP at N- terminus.DepositorInsertUBL4A (UBL4A Human)
UseTagsFLAG and GFPExpressionMammalianMutationnonePromoterCMVAvailable sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-NMHC-IIA-S1916A
Plasmid#101040PurposeExpresses GFP-MYH9 construct (human myosin IIA) carrying mutation of serine 1916 to alanine, driven by CMV promoter. Thus inhibits PKC phosphorylation of the tail. Also carries S1915A mutation.DepositorInsertNon-muscle myosin IIA heavy chain (MYH9 Human)
UseTagsGFPExpressionMammalianMutationS1916A, S1917APromoterCMVAvailable sinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-TetR-NLS-VP16
Plasmid#103834PurposeTranscription activator (transactivation domain of viral VP16 protein) that is constitutively recruited tetO arraysDepositorInsertEGFP-TetR-NLS-VP16 (tetR )
UseTagsExpressionMammalianMutationPromoterSV2Available sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMV-mEGFP-CREB (S133A)
Plasmid#137002PurposemEGFP-CREB donor for G-CREB with S133A mutationDepositorInsertCREB1 (Creb1 Rat)
UseTagsmEGFPExpressionMammalianMutationChanges Serine 133 into AlaninePromoterCMVAvailable sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-nl.EGFP-jRGECO1a
Plasmid#172386PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInsertnuclear-localized EGFP + jRGECO1a
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Akt2(K14A/R25E)
Plasmid#86595PurposeExpresses eGFP-tagged human Akt2(K14A/R25E) membrane-binding mutantDepositorInsertAkt2(K14A/R25E) (AKT2 )
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMY-eGFP-P2A-PuroR-HA
Plasmid#163343PurposeRetroviral vector to express cytosolic GFP and an HA-tagged puromycin selection markerDepositorInsertGFP
UseRetroviralTagsExpressionMammalianMutationPromoterLTRAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
Actin-53a-ires-EGFP
Plasmid#25460DepositorInsertBAF53a (Actl6a Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceSept. 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pTol-Promoter:NLS-tdMCP-eGFP
Plasmid#86248PurposeFor generating transgenic lines that ubiquitously expressing the fluorescent MCP reporterDepositorInsertbeta Actin promoter:NLS-tdMCPeGFP
UseUsed with transposase to make transient or stableā¦TagsNLS-tdMCP-eGFPExpressionMutationPromoterBeta ActinAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C3-PLK4-S305A-3xFLAG
Plasmid#69839PurposeThis plasmid encodes PLK4 isoform 1 carrying an S305A mutation with an N-terminal EGFP tag and C-terminal 3xFLAG tagDepositorInsertPLK4 (PLK4 Human)
UseTags3xFLAG tag and EGFPExpressionMammalianMutationS305APromoterCMVAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;tmem88aCE1-P1Egfp
Plasmid#90148Purposecontains tmem88a enhancer sequence, and tmem88a specific gene promoter driving expression of eGFPDepositorInserttmem88aE1P1Egfp (tmem88a Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCK072 p5E 147bp exorh:EGFP
Plasmid#195950PurposeGateway p5E vector with reporterDepositorInsertexorh minimal regulatory elements and EGFP
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only