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Plasmid#105423PurposeSynthetic biologyDepositorInsertNuclear targeting factor (RanGAP1(WPP)-GFP-BLRP)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
plenti hSyn GAPDH
Plasmid#220918PurposeNeuronal specific expression of GAPDHDepositorInsertGAPDH (GAPDH Human)
UseLentiviralTagsExpressionMammalianMutationPromoterhSynAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-mCherry-MinD-IRES-Puro
Plasmid#213112PurposeExpression of mCherry-MinD in mammalian cellsDepositorInsertmCherry-MinD (minD Escherichia coli)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
XRE-H2B-eGFP
Plasmid#182294PurposeFluorescent reporter for AhR activityDepositorInsertXenobiotic Response Element (CYP1A1 Human)
UseTagseGFPExpressionMammalianMutationPromoterXREAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHMGCS-Luc (aka: pES8)
Plasmid#14947DepositorInsertHMG CoA synthase promoter
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceMay 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLCO1A2_STOP
Plasmid#161342PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLCO1A2 (SLCO1A2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-SynI-CreOn-FlpOff-mNaChBac-P2A-EGFP
Plasmid#176280PurposeViral vector for co-expression of NaChBac and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.DepositorInsertmNaChBac-P2A-EGFP
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterhuman Synapsin IAvailable sinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-loxSTOPlox-LynEGFP
Plasmid#133922PurposeReporter expressing membrane targeted EGFP for visualization of neuronal morphology upon Cre-based recombinationDepositorInsertLynEGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
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BFP dest clone
Plasmid#71825PurposeLentiviral introduction of BFP reporter (Point mutation H66 in mEGFP results in BFP expression)DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAF123
Plasmid#135921PurposeAll-in-one Sleeping Beauty vector for stable transfection of eukaryotic cells with hygromycin restistance. CMV protomor drives SB100X transposase for transposon integration.DepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-RfxCas13d-NLS-HA
Plasmid#141321PurposePlasmid to carry out IVT of RfxCas13d-NLS (human codon-optimized)DepositorInsertRfx-Cas13d (NLS-RfxCas13d-NLS)
UseCRISPRTagsHA and NLSExpressionMutationPromoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8e-nSpG-P2A-EGFP (KAC984)
Plasmid#185911PurposepCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABE8e-nSpG-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…PromoterCMV and T7Available sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-RRBP1-1
Plasmid#92156PurposeCRISPR guide RNA targeting human RRBP1DepositorInsertRRBP1 sgRNA-1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDV05.0_pEF1a-iRFP720
Plasmid#200407PurposeExpresses iRFP720 constitutively in mammalian cellsDepositorInsertiRFP720
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-iGFP-TLG1
Plasmid#105261PurposePlasmid for pop-in/pop-out replacement of Tlg1 with iGFP-Tlg1.DepositorInsertTlg1 (TLG1 Budding Yeast)
UseTagsiGFPExpressionYeastMutationPromoterAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBEAST-pBen-Luc
Plasmid#122694PurposeOutput expression of firefly luciferase under the activation of BenR transcription factor for cell-free expressionDepositorInsertFirefly luciferase
UseSynthetic Biology; Cell-free protein synthesisTagsExpressionMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-hSyn-EGFP
Plasmid#114215PurposeLentiviral vector (control) that targets expression of EGFP to neuronsDepositorInsertEnhanced Green Fluorescent Protein
UseLentiviral and Mouse TargetingTagsNoneExpressionMammalianMutationNonePromoterhuman synapsin (hSyn)Available sinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCBC-MT2T3
Plasmid#50594PurposeCRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sitesDepositorInsertT2, gRNA scaffold, TaU3t plus, U6-26p, T3
UseCRISPR; Pcr templateTagsExpressionPlantMutationPromoterAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDSHA-SARA-Myc
Plasmid#67368PurposeMammalian expression of hSara with C-term Myc tagDepositorInsertSARA (SAR1B Human)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAC1802_pmax-RBFOX1N-dCasRx-C
Plasmid#118635PurposeTransient transfection; Expresses RBFOX1N-dCasRx-C; CAGGS promoterDepositorInsertRBFOX1N-dCasRx-C
UseCRISPRTagsExpressionMammalianMutationPromoterCAGGSAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLentiCMV-Blast
Plasmid#125133PurposeLentiviral empty backbone control vector with CMV promoter and Blasticidin resistance geneDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
RCAS cVax (CC#337)
Plasmid#15136DepositorInsertcVax (VAX1 Chicken)
UseRetroviral; Avian expressionTagsExpressionMutationPromoterAvailable sinceJune 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-SARA
Plasmid#67473PurposeMammalian expression of hSara with stop codon (native expression)DepositorInsertSARA (SAR1B Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMIY-mETV3-Flag
Plasmid#34579DepositorInsertEtv3 (Etv3 Mouse)
UseRetroviralTagsFLAG and IRES-YFPExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-OTUB1
Plasmid#22551DepositorInsertOTUB1 (OTUB1 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceNov. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAC-GAMMAipi
Plasmid#53278PurposeHas ctrE, crtB, crtI, crtY, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and crtYm gene of marine bacterium P99-3. and produces gamma-carotene in Escherichia coliDepositorInsertcrtYm
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promoterAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDF-GFPplus
Plasmid#172718PurposeExpression of a fast folding GFP variant GFP plusDepositorInsertGFP plus
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
Str-Golgin84_VSVG-SBP-EGFP
Plasmid#65305Purposesynchronize trafficking of Golgin-84 from the Golgi apparatus (RUSH system)DepositorInsertGolgin-84 (GOLGA5 Human)
UseTagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
TFORF2161
Plasmid#141950PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertGRHL1 (GRHL1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMA3790
Plasmid#70110PurposemtDNA elimination/production of rho-0 cellsDepositorInsertsEGFP
hUNG1 Y147A
Neo
UseTagsNone, human OTC mitochondrial targeting sequence,…ExpressionMammalianMutationY147A, aa's 1-77 removedPromoterCMV, EF1a, and SV40 and nativeAvailable sinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGreenFire_blastocidin
Plasmid#174103PurposepGreenFire MPRA plasmid for cloningDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD2_miniluc_newP7
Plasmid#174105Purposeadding the mini luciferase and mini promoter to the MPRA lenti constructDepositorInsertmini luciferase mini-promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
hfDECOR-F148A
Plasmid#219546PurposeExpression of CMV-TadA8e-F148A-NLS-dCas13d-NLS in mammalian cells (hfDECOR-F148A expression)DepositorInserthfDECOR-F148A
UseCRISPRTagsExpressionMammalianMutationChanged TadA8e Phenylalanine 148 to AlaninePromoterCMVAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
DECOR
Plasmid#219545PurposeExpression of CMV-TadA8e-NLS-dCas13d-NLS in mammalian cells (DECOR expression)DepositorInsertDECOR
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
hfDECOR-V106G
Plasmid#219547PurposeExpression of CMV-TadA8e-V106G-NLS-dCas13d-NLS in mammalian cells (hfDECOR-V106G expression)DepositorInserthfDECOR-V106G
UseCRISPRTagsExpressionMammalianMutationChanged TadA8e Valine 106 to GlycinePromoterCMVAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
CasRx gRNA targeting EZH2
Plasmid#219655PurposehU6-driven expression of CasRx guide RNA targeting EZH2. 5' processed DR followed by EZH2 guide.DepositorInsertCasRx gRNA targeting EZH2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
FKBP-eGFP-TAZ
Plasmid#221213Purposepart A of TAZ-SPARK-OFFDepositorInsertFKBP-eGFP-TAZ
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
HyperNova/pRSETB
Plasmid#221456PurposeFast-maturating photosensitizing fluorescent protein for chromophore-assisted light inactivationDepositorInsertHyperNova
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
T7-LacO-LgBiT
Plasmid#216779PurposeExpresses LgBiT in bacteriaDepositorInsertLgBiT
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only